Bioactive metabolites from germinated Cajanus cajan (L.) Millsp. seeds after treating with different concentrations of salicylic acid

Abstract

Salicylic acid (SA) is an effective elicitor for enhancing product formation in various agricultural practices. This study examined the diverse responses to physiological metabolites and the pathway modifications in broad-spectrum resistance-1 (BSR1) of Cajanus cajan after treatment with SA using a metabolomics technique. The significance of the SA function at the metabolite level was examined by treating C. cajan with various concentrations of SA and germinated by soaking in water for different time periods. The secondary metabolites were recovered and investigated by gas chromatography-mass spectrometry for all the periodic conditions. Chemometric analysis of the collected samples showed that the seeds responded to the SA treatment. Acetic acid increased in germinated seeds after the SA treatment. In addition, the up-regulated metabolite production was downregulated in the C. cajan seeds before germination. The levels of metabolites, including hyacinthin, furaneol, citramalic acid, palmitate, stearate, linoleate, tocopherol, glucobrassicin, syringol, and hydroxy acetophenone, were increased after the SA treatment compared to control. Hence, the SA-treated seedling is a potential bio-factory for nutraceutical products to provide significant health benefits to the human population.

HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC‐MS/MS peptide mass fingerprinting

“…10 The superior methods are used in identifying the presence of secondary metabolites functional peptides against optimized digestion conditions, such as substrate concentration, pH, hydrolysis period, temperature, and enzyme/substrate ratio, that simulate the standard functionality of the human body conditions and will be the evolving research in the future. [11][12][13] The "accidents of chemistry" lead to the constant production of oxygen-derived species such as free radicals. When the rate of free radical production increases much more quickly than the rate of antioxidant defenses, it results in oxidative stress.…”

“…Recent research is focused on the study of the potential activity of the peptides in the therapeutic field in the scavenging activity of the free radical, which promises to develop a peptide‐based drug 10 . The superior methods are used in identifying the presence of secondary metabolites functional peptides against optimized digestion conditions, such as substrate concentration, pH, hydrolysis period, temperature, and enzyme/substrate ratio, that simulate the standard functionality of the human body conditions and will be the evolving research in the future 11–13 …”