Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity

Abstract

The strain VUK-A was isolated from a sediment sample of the Coringa mangrove ecosystem was identified as Streptomyces cheonanensis based on morphological, physiological, biochemical and molecular properties. Chemical investigation of the secondary metabolites of the strain Streptomyces cheonanensis VUK-A has led to the segregation of two bioactive compounds, namely 2-Methyl butyl propyl phthalate (1) and Diethyl phthalate (2) using column chromatography. The chemical structure of the active compounds was established on the basis of spectroscopic analysis, including 1H NMR and 13C NMR spectroscopies, FTIR and EIMS. The antimicrobial activity of the bioactive compounds produced by the strain was tested against a wide variety of bacteria and fungi and expressed in terms of minimum inhibitory concentration. The compounds (1&2) were active against all the bacteria tested, and the best activity of compound 1 was recorded against Proteus vulgaris (4 µg/ml). Compounds (1&2) were active against dermatophytes and fungi but compound 1 displayed high antifungal activity against Candida albicans (8 µg/ml) and Fusarium solani (16 µg/ml) compared to standard antifungal agents. The cytotoxicity of the bioactive compound 1 was tested against MDA-MB-231, OAW-42, HeLa, and MCF-7 cell lines. The highest activity of 100 µM by compound 1 was recorded against HeLa cancer cell lines. In fact, this is the first report of 2-Methyl butyl propyl phthalate from the genus Streptomyces.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0398-6) contains supplementary material, which is available to authorized users.

Purification and cytotoxic assays of four antimicrobial metabolites extracted from Actinomycetes of the soils of Menengai Crater, Kenya

“…Ilayaraja et al (2014) reported that zones of inhibition exhibited by antimicrobials from actinomycetes were determined by the strains of the actinomycetes from which they were obtained. However, Mangamuri et al, (2016) pointed that the environment in which the actinomycetes grow determines their biochemical activities, a factor that influences the types of the produced antimicrobials.…”

“…Moreover, actinomycetes are the leading producers of antitumor agents, enzymes, immunosuppressive agents, antifungals, neurigenics, antioxidants, anti-helmitics, anti-malarials, anti-algals, and anti-inflammatory agents (Mangamuri et al, 2016). Currently, researchers are targeting the use of antibiotics from actinomycetes in other areas such as boosting immunity in AIDS and Alzheimer diseases, in addition to their use as ant-ageing agents (Bandari et al, 2017).…”

Bioactive Molecule from Streptomyces sp. Mitigates MDR Klebsiella pneumoniae in Zebrafish Infection Model

“…were isolated by serially diluting the pretreated rhizosphere soil sample and inoculating by spread plate method onto various agar medium recommended for Actinomycetes spp. such as glycerol asparagine agar, actinomycetes isolation agar (Nanjwade et al, 2010), tryptone yeast extract medium (ISP1), yeast extract malt extract agar (ISP2) (Busarakam et al, 2014) and Czapek dox agar (Mangamuri et al, 2016). The media were supplemented with gentamicin (1 μg/ml) and fluconazole (50 μg/ml) after sterilization to inhibit the growth of bacteria and fungi, respectively (Eccleston et al, 2008).…”

Antibiotics Development and the Potentials of Marine-Derived Compounds to Stem the Tide of Multidrug-Resistant Pathogenic Bacteria, Fungi, and Protozoa

“…Anti-candida activities against C. albicans ATCC 10231 have been reported for metabolites produced by Pseudonocardia endophytica VUK-10 [24], S. cheonanensis VUK-A [25] (India), and Micromonospora sp. strain G019 [26] (Vietnam).…”