Bioactive metabolites produced from Streptomyces enissocaesilis SSASC10 against fish pathogens

Shanmugam, Kumaran; Bharathi, S.; Uttra, Vairagkar; Thirunavukkarasu, Rajasekar; Nainangu, Prasannabalaji; Krishnan, Venugopal Gopi; Renuga, Pugazhvendan Sampath; Aruni, Wilson; Deivasigamani, B.

doi:10.1016/j.bcab.2020.101802

Cited by 12 publications

(9 citation statements)

References 17 publications

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“…Besides, recent literatures have been evidence in bioprospecting for natural products derived from plants [154,155] , microbial origins [156][157][158] or animals with potential antimicrobial properties to fight against multidrug Vibrio strains [159][160][161] . Streptomyces sp., a soil derived bacteria has exhibited valuable properties to be biocontrol agent of Vibrio [150,[162][163][164][165] and as probiotic in aquculture or animal husbandry [166][167][168][169][170] . These natural bacteria are safer and does not couse any resistance traits similar to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

Venggadasamy

Loh²,

Law

et al. 2021

Prog Micobes Mol Biol

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Vibrio parahaemolyticus is one of the major foodborne pathogens owing to its cause of infectious diseases such as gastroenteritis. These diseases are often associated with the consumption of contaminated seafood. This study aims to investigate the presence of V. parahaemolyticus, their virulence, antibiotic profiles, and plasmid profiles from 77 different kinds of shellfish samples collected from wet markets and supermarkets in Selangor, Malaysia. High densities of Vibrio species ( > 5 log CFU/g) were found in 14/16 groups of shellfish. Among 77 presumptive V. parahaemolyticus isolates, 43 (55.8%) were positive for the toxR gene, confirming the identity of the isolates at the species level. However, none of the V. parahaemolyticus isolates harboured the virulence tdh and trh genes. The antibiotic susceptibility of the V. parahaemolyticus isolates revealed that most of them were resistant to ampicillin (95.3%), ampicillin-sulbactam (81.4%), cefotaxime (37.2%) and imipenem (23.3%). The plasmid profiles of the V. parahaemolyticus isolates showed that 41.9% (18/43) possess at least one plasmid. Our results indicate the V. parahaemolyticus isolates are continuously exposed to various antibiotics in the environments, thus consuming the seafood carries a potential health risk to consumers. The antibiotic resistance conferred by the species necessitates an immediate plan to approach the usage of antibiotics differently.

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Section: Discussionmentioning

confidence: 99%

Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

Venggadasamy

Loh²,

Law

et al. 2021

Prog Micobes Mol Biol

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“…Kral-15, and R. ruber Kral-25 belonged to the phylum Actinobacteria. Several previous researchers also reported that the members of the phylum Actinobacteria isolated from marine environments produced a large number of secondary metabolites containing bioactive compounds in the form of pigment compounds, including carotene, melanin, phenazine, pyrrole, violacein, quinone, curcumin, and quercetin (Gopikrishnan et al, 2017;Hasan et al, 2020;Kumaran et al, 2020;Velmurugan et al, 2020). Kocuria spp.…”

Section: Characteristics Of the 16s Rrna Gene In Pigmented Heterotrop...mentioning

confidence: 98%

Antibacterial Activity of Carotenoid Pigments Produced by Heterotrophic Bacteria from Seawater in Krakal Coastal Area, Yogyakarta, Indonesia

Sidin

Retnaningrum

2022

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Investigating natural pigments resulting from marine bacterial secondary metabolites is important because of their broad benefits in the food, pharmaceutical, cosmetic, and textile industries. In this study, 52 isolates of pigmented bacteria were isolated from seawater in the Krakal coastal area, Yogyakarta, Indonesia. Seven bacterial isolates showed high pigment stability. Profiling of pigment extracts using a mixture of acetone and methanol with UV–Vis spectrophotometric reading showed that the compounds produced were carotenoids. The highest total carotenoid content with UV–Vis spectrophotometric reading at 480 nm was produced by the Kral-3 isolate (1800 mg/g), followed by Kral-15 (1698.9 mg/g) and Kral-25 (797.2 mg/g) isolates. Analysis of the antibacterial activity of the carotenoid extracts of Kral-3, Kral-15, and Kral-25 isolates using the agar well diffusion method revealed a very strong inhibition score against Escherichia coli. In addition, the carotenoid extract of the Kral-3 isolate showed a strong inhibition score against Staphylococcus aureus, whereas the Kral-15 and Kral-25 isolates showed moderate inhibition scores. Based on the 16S rRNA gene analysis, the Kral-3, Kral-15, and Kral-25 isolates were identified as Kocuria rhizophilla, Calidifontibacter sp., and Rhodococcus ruber, respectively. Based on the findings, the novel bacterial strains are a potential bioresource for the commercial production of natural carotenoids.

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“…FPO6 causing disease in ornamental fish (Carassius auratus) [37]. Kumaran et al found that the bioactive metabolite Pyrrolo [1,2-a] pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl) biosynthesized from Streptomyces enissocaesilis SSASC10 was able to against Aeromonas hydrophila causing disease in fish [38]. This study shows that Streptomyces strains TM1, TM7 and TM22 play an important role in controlling total Aeromonas count in culture water and in the intestinal tract of discus fish.…”

Section: Bacteria Countsmentioning

confidence: 99%

Dual Role Of Streptomyces Strains: Reduction of Aeromonas Counts In Both Culture Water And The Intestine, And Growth Enhancement of Discus Fish (Symphysodon sp.)

Phuc,

Huong,

Dan

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Discus fish (Symphysodon sp.) brings the largest export turnover to the ornamental fish industry in Vietnam. The intensive farming of discus fish to meet export market demand leads to a higher risk of bacterial disease, with Aeromonas being the main infectious agent. Continuous antibiotic use exerts selective pressure, fostering the emergence of antibiotic-resistant bacterial strains capable of causing diseases in fish. Streptomyces sp. considered as probiotics with the role of replacing antibiotics in applications such as promoting growth, improving survival rates, and controlling pathogenic bacteria in aquaculture. The primary objective of this study was to assess the influence of Streptomyces sp. on the growth performance, and Aeromonas density in intestine of discus fish and in culture water. Discus fish fed diets containing Streptomyces sp. TM1, TM7, and TM22 showed significantly higher growth parameters (45.24 - 58.72% of final body weight - FBW, 14.8 - 16.66% of final body length -FBL, 16.03 – 20.87 % of specific growth rate of weight – SGRW, 10.79 - 12.05 % of specific growth rate of length – SGRL, and 41.48 – 46.54 % of feed conversion ratio – FCR) compared to the control. Additionally, total Aeromonas densities of the fish treated with TM1, TM7, and TM22 showed a notable decrease compared to the control treatment in the rearing water with log CFU/ml values of 4.517 (Control), 2.554 (TM1), 2.571 (TM7), 2.222 (TM22) and in the intestines of fish with log CFU/g values of 5.597 (Control), 3.828 (TM1), 3.806 (TM7), 3.648 (TM22). Streptomyces sp. TM1, TM7, and TM22 could decreased total Aeromonas count in the gut of discus fish and in the rearing water, and boost the growth performance of discus fish (Symphysodon sp.).

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Bioactive metabolites produced from Streptomyces enissocaesilis SSASC10 against fish pathogens

Cited by 12 publications

References 17 publications

Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

Antibacterial Activity of Carotenoid Pigments Produced by Heterotrophic Bacteria from Seawater in Krakal Coastal Area, Yogyakarta, Indonesia

Dual Role Of Streptomyces Strains: Reduction of Aeromonas Counts In Both Culture Water And The Intestine, And Growth Enhancement of Discus Fish (Symphysodon sp.)

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