Bioactive micropatterning of apatite immobilizing cell adhesion protein by laser-induced forward transfer with a shock absorber

Narazaki, Aiko; Oyane, Ayako; Komuro, Saki; Kurosaki, Ryozo; Kameyama, Tomoko; Sakamaki, Ikuko; Araki, Hideki; Miyaji, Hirofumi

doi:10.1364/ome.9.002807

Cited by 14 publications

(19 citation statements)

References 33 publications

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“…This suggests that cracks might be generated by freezedrying in the Fn-CaP film on the C/PDMS/PET support most likely due to the relatively high coefficient of thermal expansion of PDMS. The high-magnification SEM images (Figure 2b,d) showed that the Fn-CaP film surfaces have a porous structure, which is a typical morphology of CaP films prepared using the biomimetic process [16]. There is no apparent difference in the surface structure between these substrates with and without the PDMS layer.…”

Section: Cross-sectional Observation Of the Transferred Fn-cap Microcmentioning

confidence: 86%

“…Fn-CaP donor films were prepared on an optical stamp through a precursor-assisted biomimetic process [30,32], as detailed in the previous report [16]. Different from the previous work [16], a PET substrate was used as a transparent support, one-side of which was coated with a 1-mm-thick PDMS sheet. Hereafter, the PDMS-coated PET substrate is referred to as an optical stamp (denoted as PDMS/PET).…”

Section: Preparation Of Fn-cap Donor Films On Optical Stamp Using Thementioning

confidence: 99%

“…The plasma-treated C/PDMS/PET substrate was alternately dipped in 0.2 M CaCl 2 and 0.2 M K 2 HPO 4 •3H 2 O aqueous solutions for CaP precoating and, subsequently, immersed at 25 • C for 5 h in a supersaturated CaP solution for film growth. Fn (from bovine plasma, Sigma-Aldrich) was supplemented at a final concentration of 40 µg/mL to the CaCl 2 and K 2 HPO 4 •3H 2 O solutions and to the supersaturated CaP solution for Fn immobilization within the film [16]. Fn is a biofunctional protein, which promotes cell adhesion and spreading.…”

Section: Preparation Of Fn-cap Donor Films On Optical Stamp Using Thementioning

confidence: 99%

“…While most of them are laser removal and surface modification technologies, since the 1980s, laser-induced forward transfer (LIFT) has been developed as a simple and unique additive manufacturing method [1]. By using LIFT, various substances, such as metals [2][3][4][5], semiconductors [6], oxides [7][8][9], silver nanopaste [10][11][12], graphene [13], and biomaterials [14][15][16], can be printed even at a micron/submicron resolution under atmospheric and room-temperature conditions. The LIFT process generally involves the irradiation of a single laser pulse through a transparent support onto 2 of 11 a donor material or a sacrificial layer that absorbs laser light, leading to laser-induced phenomena such as heating, melting, ablation, etc.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous work, we demonstrated the rapid printing of cell adhesion protein fibronectin (Fn)-immobilized hydroxyapatite (Fn-apatite) microchips by applying LIFT to a shock-absorbing polydimethylsiloxane (PDMS) substrate [16]. Further, the conducted CHO-K1 cell culture experiments revealed the good cytocompatibility of the Fn-apatite microchips.…”

Section: Introductionmentioning

confidence: 99%

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Laser-Induced Forward Transfer with Optical Stamp of a Protein-Immobilized Calcium Phosphate Film Prepared by Biomimetic Process to a Human Dentin

2020

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The rapid and area-specific printing of calcium phosphate with superior biocompatibility and osteoconductivity is a useful technique for the surface functionalization of biomedical devices. We recently demonstrated the laser-induced forward transfer (LIFT) of a brittle calcium phosphate film onto a soft and shock-absorbing polydimethylsiloxane (PDMS) substrate. In this work, a new LIFT using an optically transparent PDMS-coated stamp, which we hereafter call LIFT with optical stamp (LIFTOP), was introduced to achieve the transfer of brittle films to harder substrates. Cell adhesion protein fibronectin-immobilized calcium phosphate films (Fn-CaP) were prepared on the optical stamp through a biomimetic process. Then, the irradiation of a single laser pulse transferred the Fn-CaP film from the optical stamp onto relatively hard substrates, polyethylene terephthalate and human dentin. As a result of this LIFTOP process, Fn-CaP microchips with a shape corresponding to the laser beam spot were printed on the substrates. Cross-sectional observation of the interface between the Fn-CaP microchip and the dentin substrate revealed good attachment between them without obvious gaps for the most part.

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Section: Cross-sectional Observation Of the Transferred Fn-cap Microcmentioning

confidence: 86%

Section: Preparation Of Fn-cap Donor Films On Optical Stamp Using Thementioning

confidence: 99%

Section: Preparation Of Fn-cap Donor Films On Optical Stamp Using Thementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Laser-Induced Forward Transfer with Optical Stamp of a Protein-Immobilized Calcium Phosphate Film Prepared by Biomimetic Process to a Human Dentin

2020

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Printed Electronics Technologies for Additive Manufacturing of Hybrid Electronic Sensor Systems

Zikulnig

Chang

Bito³

et al. 2023

Advanced Sensor Research

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Requirements for the miniaturization of electronics are constantly increasing as more and more functions are aimed to be integrated into a single device. At the same time, there are strong demands for low-cost manufacturing, environmental compatibility, rapid prototyping, and small-scale productions due to fierce competition, policies, rapid technical progress, and short innovation times. Altogether, those challenges cannot be sufficiently addressed by simply using either printed or silicon electronics. Instead, the synergies from combining those two technologies into so-called hybrid electronics create novel opportunities for advanced capabilities and new areas of applications. In the first part of this review, printing and patterning technologies are presented with potential compatibility with conventional electronics manufacturing techniques. They can be utilized for the fabrication of highly complex structures. Nonetheless, up-scalability, integration, and adaptation for industrial fabrication remain challenging due to technically limiting factors. Consequently, a special focus is placed on the up-scalability, availability of commercial printing, and manufacturing machines, as well as processing challenges for high-volume industrial applications. The second part of this review further provides an overview of exciting and innovative application possibilities of printed electronics, emphasizing sensor applications, as well as additively manufactured integrated circuits.

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Characterization of Laser-Processed Samples

Narazaki¹

2021

Handbook of Laser Micro- And Nano-Engineering

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Bioactive micropatterning of apatite immobilizing cell adhesion protein by laser-induced forward transfer with a shock absorber

Cited by 14 publications

References 33 publications

Laser-Induced Forward Transfer with Optical Stamp of a Protein-Immobilized Calcium Phosphate Film Prepared by Biomimetic Process to a Human Dentin

Laser-Induced Forward Transfer with Optical Stamp of a Protein-Immobilized Calcium Phosphate Film Prepared by Biomimetic Process to a Human Dentin

Printed Electronics Technologies for Additive Manufacturing of Hybrid Electronic Sensor Systems

Characterization of Laser-Processed Samples

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