Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski, Tomasz; Kijeńska‐Gawrońska, Ewa; Zołocińska, Aleksandra; Siennicka, Katarzyna; Słysz, Anna; Paskal, Wiktor; Włodarski, Paweł; Święszkowski, Wojciech; Pojda, Zygmunt

doi:10.3390/ijms22115588

Cited by 19 publications

(19 citation statements)

References 55 publications

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“…Furthermore, no significant differences were found among the DPNAs studied. These results agree with most articles in which better muscle trophism was obtained in animals treated with nerve autografts than bioengineered nerve substitutes ( Debski et al, 2021 ; Yu et al, 2021 ) and DPNAs ( Zheng et al, 2014 ; Chato-Astrain et al, 2020b ; Qian et al, 2022 ).…”

Section: Discussionsupporting

confidence: 91%

Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts

García-García

Soury²,

Campos³

et al. 2023

Front. Bioeng. Biotechnol.

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As a reliable alternative to autografts, decellularized peripheral nerve allografts (DPNAs) should mimic the complex microstructure of native nerves and be immunogenically compatible. Nevertheless, there is a current lack of decellularization methods able to remove peripheral nerve cells without significantly altering the nerve extracellular matrix (ECM). The aims of this study are firstly to characterize ex vivo, in a histological, biochemical, biomechanical and ultrastructural way, three novel chemical-enzymatic decellularization protocols (P1, P2 and P3) in rat sciatic nerves and compared with the Sondell classic decellularization method and then, to select the most promising DPNAs to be tested in vivo. All the DPNAs generated present an efficient removal of the cellular material and myelin, while preserving the laminin and collagen network of the ECM (except P3) and were free from any significant alterations in the biomechanical parameters and biocompatibility properties. Then, P1 and P2 were selected to evaluate their regenerative effectivity and were compared with Sondell and autograft techniques in an in vivo model of sciatic defect with a 10-mm gap, after 15 weeks of follow-up. All study groups showed a partial motor and sensory recovery that were in correlation with the histological, histomorphometrical and ultrastructural analyses of nerve regeneration, being P2 the protocol showing the most similar results to the autograft control group.

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Section: Discussionsupporting

confidence: 91%

Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts

García-García

Soury²,

Campos³

et al. 2023

Front. Bioeng. Biotechnol.

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“…For example, Debski et al examined a nanofiber-based nerve conduit composed of poly (L-lactic acid)-co-poly(caprolactone), collagen and polyaniline (PANI) in a rat model. This conduit presented muscle atrophy decrease and was suggested as a mean for axonal regeneration support and managing nerve gap as in peripheral nerve [24]. Polycaprolactone/collagen VI electrospun conduits were assessed by Lv et al in a 15-mm-long sciatic nerve defect in rats and reported the sustained release of collagen VI enhanced the recruitment of macrophages and their polarization toward the pro-healing (M2) phenotype [25].…”

Section: Application Of Electrospun Substrates For Repairing the Nervous Systemmentioning

confidence: 99%

Advances in Electrospun Nerve Guidance Conduits for Engineering Neural Regeneration

2022

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Injuries to the peripheral nervous system result in devastating consequences with loss of motor and sensory function and lifelong impairments. Current treatments have largely relied on surgical procedures, including nerve autografts to repair damaged nerves. Despite improvements to the surgical procedures over the years, the clinical success of nerve autografts is limited by fundamental issues, such as low functionality and mismatching between the damaged and donor nerves. While peripheral nerves can regenerate to some extent, the resultant outcomes are often disappointing, particularly for serious injuries, and the ongoing loss of function due to poor nerve regeneration is a serious public health problem worldwide. Thus, a successful therapeutic modality to bring functional recovery is urgently needed. With advances in three-dimensional cell culturing, nerve guidance conduits (NGCs) have emerged as a promising strategy for improving functional outcomes. Therefore, they offer a potential therapeutic alternative to nerve autografts. NGCs are tubular biostructures to bridge nerve injury sites via orienting axonal growth in an organized fashion as well as supplying a supportively appropriate microenvironment. Comprehensive NGC creation requires fundamental considerations of various aspects, including structure design, extracellular matrix components and cell composition. With these considerations, the production of an NGC that mimics the endogenous extracellular matrix structure can enhance neuron–NGC interactions and thereby promote regeneration and restoration of function in the target area. The use of electrospun fibrous substrates has a high potential to replicate the native extracellular matrix structure. With recent advances in electrospinning, it is now possible to generate numerous different biomimetic features within the NGCs. This review explores the use of electrospinning for the regeneration of the nervous system and discusses the main requirements, challenges and advances in developing and applying the electrospun NGC in the clinical practice of nerve injuries.

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“…However, in case of the nerve gap is over than 5 mm, incomplete regeneration can be occurred. To overcome this incomplete regeneration, different growth factors, cells, and modifications of the internal framework should be examined [ 28 , 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Pre-Induced Mesenchymal Stem Cell-Coated Cellulose/Collagen Nanofibrous Nerve Conduit on Regeneration of Transected Facial Nerve

Cho

Moon

Maharajan

et al. 2022

IJMS

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(1) Objective: In order to evaluate the effect of a pre-induced mesenchymal stem cell (MSC)-coated cellulose/collagen nanofibrous nerve conduit on facial nerve regeneration in a rat model both in vitro and in vivo. (2) Methods: After fabrication of the cellulose/collagen nanofibrous conduit, its lumen was coated with either MSCs or pre-induced MSCs. The nerve conduit was then applied to the defective main trunk of the facial nerve. Rats were randomly divided into three treatment groups (n = 10 in each): cellulose/collagen nanofiber (control group), cellulose/collagen nanofiber/MSCs (group I), and cellulose/collagen nanofiber/pre-induced MSCs (group II). (3) Results Fibrillation of the vibrissae of each group was observed, and action potential threshold was compared 8 weeks post-surgery. Histopathological changes were also observed. Groups I and II showed better recovery of vibrissa fibrillation than the control group. (4) Conclusions: Group II, treated with the pre-induced MSC-coated cellulose/collagen nanofibrous nerve conduit, showed the highest degree of recovery based on functional and histological evaluations.

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Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Cited by 19 publications

References 55 publications

Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts

Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts

Advances in Electrospun Nerve Guidance Conduits for Engineering Neural Regeneration

Effect of Pre-Induced Mesenchymal Stem Cell-Coated Cellulose/Collagen Nanofibrous Nerve Conduit on Regeneration of Transected Facial Nerve

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