Bioactive nanoparticle reinforced alginate/gelatin bioink for the maintenance of stem cell stemness

Li, Jianjun; Zhang, Yijie; Jirigala, Enhe; Yao, Bin; Wang, Yuzhen; Zhu, Daoyu; Zhao, Li; Song, Wei; Duan, Xianlan; Yuan, Xingyu; Fu, Xiaobing; Huang, Sha

doi:10.1016/j.msec.2021.112193

Cited by 41 publications

(29 citation statements)

References 27 publications

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“…Astonishingly, hydrogel stiffness has also been demonstrated to affect cell activities and functions, such as cellular morphology, 158 proliferation, 159 migration, 160 differentiation, 161 stemness, 162 etc. Hence, it is necessary to comprehensively understand how cells sense and respond to hydrogel stiffness, which could navigate the future design of hydrogels.…”

Section: Influences Of Hydrogel Properties On Cell Behavior and Signaling Pathwaysmentioning

confidence: 99%

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Cao

Duan

Yan

et al. 2021

Sig Transduct Target Ther

480

256

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Hydrogel is a type of versatile platform with various biomedical applications after rational structure and functional design that leverages on material engineering to modulate its physicochemical properties (e.g., stiffness, pore size, viscoelasticity, microarchitecture, degradability, ligand presentation, stimulus-responsive properties, etc.) and influence cell signaling cascades and fate. In the past few decades, a plethora of pioneering studies have been implemented to explore the cell–hydrogel matrix interactions and figure out the underlying mechanisms, paving the way to the lab-to-clinic translation of hydrogel-based therapies. In this review, we first introduced the physicochemical properties of hydrogels and their fabrication approaches concisely. Subsequently, the comprehensive description and deep discussion were elucidated, wherein the influences of different hydrogels properties on cell behaviors and cellular signaling events were highlighted. These behaviors or events included integrin clustering, focal adhesion (FA) complex accumulation and activation, cytoskeleton rearrangement, protein cyto-nuclei shuttling and activation (e.g., Yes-associated protein (YAP), catenin, etc.), cellular compartment reorganization, gene expression, and further cell biology modulation (e.g., spreading, migration, proliferation, lineage commitment, etc.). Based on them, current in vitro and in vivo hydrogel applications that mainly covered diseases models, various cell delivery protocols for tissue regeneration and disease therapy, smart drug carrier, bioimaging, biosensor, and conductive wearable/implantable biodevices, etc. were further summarized and discussed. More significantly, the clinical translation potential and trials of hydrogels were presented, accompanied with which the remaining challenges and future perspectives in this field were emphasized. Collectively, the comprehensive and deep insights in this review will shed light on the design principles of new biomedical hydrogels to understand and modulate cellular processes, which are available for providing significant indications for future hydrogel design and serving for a broad range of biomedical applications.

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Section: Influences Of Hydrogel Properties On Cell Behavior and Signaling Pathwaysmentioning

confidence: 99%

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Cao

Duan

Yan

et al. 2021

Sig Transduct Target Ther

480

256

View full text Add to dashboard Cite

show abstract

“…to the hydrogel, the mechanical properties and bioactivity have been significantly improved [ 68 ]. Furthermore, due to the unique microstructure and chemical composition of these inorganic fillers, these materials are able to promote cell adhesion, proliferation and some of them even enable to regulate the cell fate of stem cells or progenitor cells [ 69 ].…”

Section: Strategies For Improving the Bioink Performancesmentioning

confidence: 99%

“…Interestingly, bioactive nanoparticles did not change pore properties of the bioink and the influence on stem cell regulation is attributed to the biochemical signals of bioactive nanoparticles ( Fig. 4 ) [ 69 ]. Zhai et al [ 74 ] mixed laponite nanoclay in bioinks which could release Mg 2+ and Si 4+ to support osteogenic ability in vitro and in vivo .…”

Section: Strategies For Improving the Bioink Performancesmentioning

confidence: 99%

Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Wang

Yuan

Yao

et al. 2022

Bioactive Materials

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“…Stiffer hydrogels were known to enhance cytoskeletal organization, thus increasing osteoblastic/odontogenic differentiation and proliferation of stem cells [34]. Furthermore, numerous results had proved that alginate coupled with RGD motifs such as gelatin significantly enhanced cellular adhesion, migration, proliferation, and differentiation as alginate itself does not promote cellular activities due to the lack of cellular adhesion motifs [35]. In addition, the increasing Si concentration had a positive impact on the mechanical properties of the FGelMa-based hydrogels (Figure 3B).…”

Section: Synthesis and Characterization Of The Fgelma And Alg/gel Hydrogelsmentioning

confidence: 99%

Synergies of Human Umbilical Vein Endothelial Cell-Laden Calcium Silicate-Activated Gelatin Methacrylate for Accelerating 3D Human Dental Pulp Stem Cell Differentiation for Endodontic Regeneration

et al. 2021

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According to the Centers for Disease Control and Prevention, tooth caries is a common problem affecting 9 out of every 10 adults worldwide. Dentin regeneration has since become one of the pressing issues in dentistry with tissue engineering emerging as a potential solution for enhancing dentin regeneration. In this study, we fabricated cell blocks with human dental pulp stem cells (hDPSCs)-laden alginate/fish gelatin hydrogels (Alg/FGel) at the center of the cell block and human umbilical vascular endothelial cells (HUVEC)-laden Si ion-infused fish gelatin methacrylate (FGelMa) at the periphery of the cell block. 1H NMR and FTIR results showed the successful fabrication of Alg/FGel and FGelMa. In addition, Si ions in the FGelMa were noted to be bonded via covalent bonds and the increased number of covalent bonds led to an increase in mechanical properties and improved degradation of FGelMa. The Si-containing FGelMa was able to release Si ions, which subsequently significantly not only enhanced the expressions of angiogenic-related protein, but also secreted some cytokines to regulate odontogenesis. Further immunofluorescence results indicated that the cell blocks allowed interactions between the HUVEC and hDPSCs, and taken together, were able to enhance odontogenic-related markers’ expression, such as alkaline phosphatase (ALP), dentin matrix phosphoprotein-1 (DMP-1), and osteocalcin (OC). Subsequent Alizarin Red S stain confirmed the benefits of our cell block and demonstrated that such a novel combination and modification of biomaterials can serve as a platform for future clinical applications and use in dentin regeneration.

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Bioactive nanoparticle reinforced alginate/gelatin bioink for the maintenance of stem cell stemness

Cited by 41 publications

References 27 publications

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing

Synergies of Human Umbilical Vein Endothelial Cell-Laden Calcium Silicate-Activated Gelatin Methacrylate for Accelerating 3D Human Dental Pulp Stem Cell Differentiation for Endodontic Regeneration

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