Bioactive Oxylipins Profile in Marine Microalgae

Linares-Maurizi, Amandyne; Réversat, Guillaume; Awad, Rana; Bultel-Poncé, Valérie; Oger, Camille; Galano, Jean‐Marie; Balas, Laurence; Durbec, Anaelle; Bertrand‐Michel, Justine; Pradelles, Rémi; Vigor, Claire

doi:10.3390/md21030136

Cited by 9 publications

(7 citation statements)

References 61 publications

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“…Another way that oxygen could influence the fatty acid profile is the enzymatic or non-enzymatic oxidation of PUFAs to oxylipins, a class of molecules that act as mediators and have bioactive properties beneficial to human health [ 36 ]. Oxidative conditions such as those present under elevated oxygen concentration and saturating light intensity could enhance oxylipin synthesis, which could cause an observed reduction in the abundance of the respective fatty acid.…”

Section: Discussionmentioning

confidence: 99%

LASSO Regression with Multiple Imputations for the Selection of Key Variables Affecting the Fatty Acid Profile of Nannochloropsis oculata

Andriopoulos,

Kornaros

2023

Marine Drugs

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The marine microalga Nannochloropsis oculata has garnered significant interest as a potential source of lipids, both for biofuel and nutrition, containing significant amounts of C16:0, C16:1, and C20:5, n-3 (EPA) fatty acids (FA). Growth parameters such as temperature, pH, light intensity, and nutrient availability play a crucial role in the fatty acid profile of microalgae, with N. oculata being no exception. This study aims to identify key variables for the FA profile of N. oculata grown autotrophically. To that end, the most relevant literature data were gathered and combined with our previous work as well as with novel experimental data, with 121 observations in total. The examined variables were the percentages of C14:0, C16:0, C16:1, C18:1, C18:2, and C20:5, n-3 in total FAs, their respective ratios to C16:0, and the respective content of biomass in those fatty acids in terms of ash free dry weight. Many potential predictor variables were collected, while dummy variables were introduced to account for bias in the measured variables originating from different authors as well as for other parameters. The method of multiple imputations was chosen to handle missing data, with limits based on the literature and model-based estimation, such as using the software PHREEQC and residual modelling for the estimation of pH. To eliminate unimportant predictor variables, LASSO (Least Absolute Shrinkage and Selection Operator) regression analysis with a novel definition of optimal lambda was employed. LASSO regression identified the most relevant predictors while minimizing the risk of overfitting the model. Subsequently, stepwise linear regression with interaction terms was used to further study the effects of the selected predictors. After two rounds of regression, sparse refined models were acquired, and their coefficients were evaluated based on significance. Our analysis confirms well-known effects, such as that of temperature, and it uncovers novel unreported effects of aeration, calcium, magnesium, and manganese. Of special interest is the negative effect of aeration on polyunsaturated fatty acids (PUFAs), which is possibly related to the enzymatic kinetics of fatty acid desaturation under increased oxygen concentration. These findings contribute to the optimization of the fatty acid profile of N. oculata for different purposes, such as production of, high in PUFAs, food or feed, or production of, high in saturated and monounsaturated FA methyl esters (FAME), biofuels.

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Section: Discussionmentioning

confidence: 99%

LASSO Regression with Multiple Imputations for the Selection of Key Variables Affecting the Fatty Acid Profile of Nannochloropsis oculata

Andriopoulos,

Kornaros

2023

Marine Drugs

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“…Similarly, the microalgae samples had been extracted in the framework of a previous study including the sample grinding and multiple centrifugation and purification steps 36 , 37 .…”

Section: Methodsmentioning

confidence: 99%

From MS/MS library implementation to molecular networks: Exploring oxylipin diversity with NEO-MSMS

Elloumi,

Mas-Normand,

Bride

et al. 2024

Sci Data

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Oxylipins, small polar molecules derived from the peroxidation of polyunsaturated fatty acids (PUFAs), serve as biomarkers for many diseases and play crucial roles in human physiology and inflammation. Despite their significance, many non-enzymatic oxygenated metabolites of PUFAs (NEO-PUFAs) remain poorly reported, resulting in a lack of public datasets of experimental data and limiting their dereplication in further studies. To overcome this limitation, we constructed a high-resolution tandem mass spectrometry (MS/MS) dataset comprising pure NEO-PUFAs (both commercial and self-synthesized) and in vitro free radical-induced oxidation of diverse PUFAs. By employing molecular networking techniques with this dataset and the existent ones in public repositories, we successfully mapped a wide range of NEO-PUFAs, expanding the strategies for annotating oxylipins, and NEO-PUFAs and offering a novel workflow for profiling these molecules in biological samples.

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“…PUFA and oxylipins can be used as nutricosmetics, food supplements, and feeds [ 290 , 291 ]. The cost, microbial and chemical contaminants’ accumulation, and the lack of technology viable for large-scale applications give a setback to algal wastewater treatments [ 292 ]. Different is the speech of the potential use of the beach-cast macroalgae.…”

Section: Macroalgae Biomass In a Circular Economy Perspectivementioning

confidence: 99%

The Potential of Algae in the Nutricosmetic Sector

Dini

2023

Molecules

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Seaweeds or algae are marine autotrophic organisms. They produce nutrients (e.g., proteins, carbohydrates, etc.) essential for the survival of living organisms as they participate in biochemical processes and non-nutritive molecules (such as dietary fibers and secondary metabolites), which can improve their physiological functions. Seaweed polysaccharides, fatty acids, peptides, terpenoids, pigments, and polyphenols have biological properties that can be used to develop food supplements and nutricosmetic products as they can act as antibacterial, antiviral, antioxidant, and anti-inflammatory compounds. This review examines the (primary and secondary) metabolites produced by algae, the most recent evidence of their effect on human health conditions, with particular attention to what concerns the skin and hair’s well-being. It also evaluates the industrial potential of recovering these metabolites from biomass produced by algae used to clean wastewater. The results demonstrate that algae can be considered a natural source of bioactive molecules for well-being formulations. The primary and secondary metabolites’ upcycling can be an exciting opportunity to safeguard the planet (promoting a circular economy) and, at the same time, obtain low-cost bioactive molecules for the food, cosmetic, and pharmaceutical industries from low-cost, raw, and renewable materials. Today’s lack of methodologies for recovering bioactive molecules in large-scale processes limits practical realization.

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Bioactive Oxylipins Profile in Marine Microalgae

Cited by 9 publications

References 61 publications

LASSO Regression with Multiple Imputations for the Selection of Key Variables Affecting the Fatty Acid Profile of Nannochloropsis oculata

LASSO Regression with Multiple Imputations for the Selection of Key Variables Affecting the Fatty Acid Profile of Nannochloropsis oculata

From MS/MS library implementation to molecular networks: Exploring oxylipin diversity with NEO-MSMS

The Potential of Algae in the Nutricosmetic Sector

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