Bioactive oxytocin in human and baboon corpora lutea

Khan-Dawood, Firyal S.; Yang, Jun; Anwer, Khursheed; Dawood, M. Yusoff

doi:10.1677/joe.0.1470525

Cited by 12 publications

(4 citation statements)

References 13 publications

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“…Oxytocin has also been detected in the corpus luteum (CL) (66), and the CL synthesises oxytocin (60). In addition oxytocin receptors have been detected (66,67).…”

Section: Corpus Luteummentioning

confidence: 99%

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Oxytocin in the human--regulation of derivations and destinations

Evans

1997

European Journal of Endocrinology

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“…Oxytocin has also been detected in the corpus luteum (CL) (66), and the CL synthesises oxytocin (60). In addition oxytocin receptors have been detected (66,67).…”

Section: Corpus Luteummentioning

confidence: 99%

“…In addition oxytocin receptors have been detected (66,67). However the CL responds to oxytocin in a complex manner and the role(s) of oxytocin with regard to the human CL are yet to be defined.…”

Section: Corpus Luteummentioning

confidence: 99%

Oxytocin in the human--regulation of derivations and destinations

Evans

1997

European Journal of Endocrinology

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“…Oxytocin (OT) is one such factor that is present in the steroidogenic cells of the human (Khan-Dawood 1987a) and baboon (Khan-Dawood 1987b) corpus luteum. OT receptors are present in luteal tissue ; OT is synthesized by luteal cells (Ivell et al 1990) and is biologically active (Khan-Dawood et al 1995). The levels of the peptide in the corpus luteum vary throughout the luteal phase, with maximum levels in the mid-luteal phase (Dawood & Khan-Dawood 1986, Khan-Dawood et al 1988.…”

Section: Introductionmentioning

confidence: 99%

Hormonal regulation of connexin-43 in baboon corpora lutea

Khan-Dawood

Yang²,

Dawood³

1998

Journal of Endocrinology

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The synthesis and secretion of progesterone in the corpus luteum are regulated by both endocrine and paracrine/ autocrine factors which affect the steroidogenic cells. Evidence suggests that these cells communicate via cellcell junctional proteins, the connexins. Previously we have shown that connexin-43 is expressed in both human and baboon (Papio hamadryus anubis) corpora lutea, with differential expression throughout luteal development, but is not detectable in corpora albicantia. We have examined the effect of human chorionic gonadotropin (hCG), oxytocin, clomiphene citrate and the anti-progesterone onapristone on expression of connexin-43 protein in the early luteal phase 1-5 days after the mid-cycle luteinizing hormone (LH) surge (LH+1-5 days), the mid-luteal phase 6-10 days after the LH surge (LH+6-10 days), and the late luteal phase 11-15 days after the LH surge (LH+11-15 days) in corpora lutea obtained from normal adult cycling females. Connexin-43 was localized by immunohistochemistry in cultured cells from all the three stages. Western blot analysis of the treated cells indicated the presence of two bands at 43 and 45 kDa. The band at 45 kDa was found to be phosphorylated connexin-43, indicating the presence of functional gap junctions. hCG (10 IU/ml) stimulated the expression of connexin-43 throughout luteal development; however, maximum expression occurred in the early luteal phase with a significantly greater expression of the non-phosphorylated protein. In contrast, in the mid-luteal phase, the expression of the phosphorylated protein was predominant. Oxytocin (200 mU/ml) also stimulated connexin-43 expression throughout luteal development with similar effects on the phosphorylated and non-phosphorylated protein in the early and mid-luteal phase; however, compared with hCG, oxytocin had a greater effect on mid-luteal phase connexin-43 expression. In the presence of both hCG and oxytocin, the expression of connexin-43 was significantly higher than the control only in the late luteal phase. Both clomiphene citrate and onapristone suppressed connexin-43 expression, and concomitant addition of hCG did not counteract their effect. In the context of our previous studies, it is concluded that, together with LH/hCG and the steroid hormones, oxytocin is involved in cell-cell contact-dependent communication in the corpus luteum.

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“…Furthermore, the peptide has been demonstrated to be bioactive (8). Oxytocin modulates luteal cell P production and secretion in vitro (1,9) and pulsatile P secretion by the CL (10).…”

mentioning

confidence: 99%