Bioactive Polymeric Materials for the Advancement of Regenerative Medicine

Iovene, Anthony; Zhao, Yuwen; Wang, Shue; Amoako, Kagya A.

doi:10.3390/jfb12010014

Cited by 30 publications

(22 citation statements)

References 154 publications

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“…The well-plates with the dimensions of 120 mm × 85 mm were placed on the shaker, labeled in Figure 1D . Thus, the applied shear stress to different wells ranges from 3 dyne/cm 2 to 7 dyne/cm 2 , which are similar to the values reported by others ( Dardik et al, 2005 ; Lim et al, 2014 ; Iovene et al, 2021 ) .…”

Section: Resultssupporting

confidence: 89%

Notch signaling and fluid shear stress in regulating osteogenic differentiation

Zhao

Richardson

Bousraou

et al. 2022

Front. Bioeng. Biotechnol.

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Osteoporosis is a common bone and metabolic disease that is characterized by bone density loss and microstructural degeneration. Human bone marrow-derived mesenchymal stem cells (hMSCs) are multipotent progenitor cells with the potential to differentiate into various cell types, including osteoblasts, chondrocytes, and adipocytes, which have been utilized extensively in the field of bone tissue engineering and cell-based therapy. Although fluid shear stress plays an important role in bone osteogenic differentiation, the cellular and molecular mechanisms underlying this effect remain poorly understood. Here, a locked nucleic acid (LNA)/DNA nanobiosensor was exploited to monitor mRNA gene expression of hMSCs that were exposed to physiologically relevant fluid shear stress to examine the regulatory role of Notch signaling during osteogenic differentiation. First, the effects of fluid shear stress on cell viability, proliferation, morphology, and osteogenic differentiation were investigated and compared. Our results showed shear stress modulates hMSCs morphology and osteogenic differentiation depending on the applied shear and duration. By incorporating this LNA/DNA nanobiosensor and alkaline phosphatase (ALP) staining, we further investigated the role of Notch signaling in regulating osteogenic differentiation. Pharmacological treatment is applied to disrupt Notch signaling to investigate the mechanisms that govern shear stress induced osteogenic differentiation. Our experimental results provide convincing evidence supporting that physiologically relevant shear stress regulates osteogenic differentiation through Notch signaling. Inhibition of Notch signaling mediates the effects of shear stress on osteogenic differentiation, with reduced ALP enzyme activity and decreased Dll4 mRNA expression. In conclusion, our results will add new information concerning osteogenic differentiation of hMSCs under shear stress and the regulatory role of Notch signaling. Further studies may elucidate the mechanisms underlying the mechanosensitive role of Notch signaling in stem cell differentiation.

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Section: Resultssupporting

confidence: 89%

Notch signaling and fluid shear stress in regulating osteogenic differentiation

Zhao

Richardson

Bousraou

et al. 2022

Front. Bioeng. Biotechnol.

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“…1D . Thus, the applied shear stress to different wells ranges from 3 dyne/cm 2 to 7 dyne/cm 2 , which are similar to the values reported by others [37; 38; 39].…”

Section: Resultssupporting

confidence: 88%

Notch Signaling and Fluid Shear Stress in Regulating Osteogenic Differentiation

Wang

Zhao

Richardson

et al. 2022

Preprint

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Osteoporosis is a common bone and metabolic disease that is characterized by bone density loss and microstructural degeneration. Human bone marrow-derived mesenchymal stem cells (hMSCs) are multipotent progenitor cells with the potential to differentiate into various cell types, including osteoblasts, chondrocytes, and adipocytes, which have been utilized extensively in the field of bone tissue engineering and cell-based therapy. Although fluid shear stress plays an important role in bone osteogenic differentiation, the cellular and molecular mechanisms underlying this effect remain poorly understood. Here, an LNA/DNA nanobiosensor was exploited to monitor mRNA gene expression of hMSCs that were exposed to physiologically relevant fluid shear stress to examine the regulatory role of Notch signaling during osteogenic differentiation. First, the effects of fluid shear stress on cell viability, proliferation, morphology, and osteogenic differentiation were investigated and compared. Our results showed shear stress modulates hMSCs morphology and osteogenic differentiation depending on the applied shear and duration. By incorporating this LNA/DNA nanobiosensor and alkaline phosphatase (ALP) staining, we further investigated the role of Notch signaling in regulating osteogenic differentiation. Pharmacological treatment is applied to disrupt Notch signaling to investigate the mechanisms that govern shear stress induced osteogenic differentiation. Our experimental results provide convincing evidence supporting that physiologically relevant shear stress regulates osteogenic differentiation through Notch signaling. Inhibition of Notch signaling mediates the effects of shear stress on osteogenic differentiation, with reduced ALP enzyme activity and decreased Dll4 mRNA expression. In conclusion, our results will add new information concerning osteogenic differentiation of hMSCs under shear stress and the regulatory role of Notch signaling. Further studies may elucidate the mechanisms underlying the mechanosensitive role of Notch signaling in stem cell differentiation.

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“…On the other hand, their resources could be insufficient in relation to the demand. Another problem may also be connected with poor mechanical strength and resistance [ 141 , 142 ] ( Figure 4 ).…”

Section: Natural Vs Synthetic Polymers For Dental Applicationmentioning

confidence: 99%

Natural Polymers for the Maintenance of Oral Health: Review of Recent Advances and Perspectives

Paradowska-Stolarz

Więckiewicz

Owczarek

et al. 2021

IJMS

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The success of modern dental treatment is strongly dependent on the materials used both temporarily and permanently. Among all dental materials, polymers are a very important class with a wide spectrum of applications. This review aims to provide a state-of-the-art overview of the recent advances in the field of natural polymers used to maintain or restore oral health. It focuses on the properties of the most common proteins and polysaccharides of natural origin in terms of meeting the specific biological requirements in the increasingly demanding field of modern dentistry. The use of naturally derived polymers in different dental specialties for preventive and therapeutic purposes has been discussed. The major fields of application cover caries and the management of periodontal diseases, the fabrication of membranes and scaffolds for the regeneration of dental structures, the manufacturing of oral appliances and dentures as well as providing systems for oral drug delivery. This paper also includes a comparative characteristic of natural and synthetic dental polymers. Finally, the current review highlights new perspectives, possible future advancements, as well as challenges that may be encountered by researchers in the field of dental applications of polymers of natural origin.

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Bioactive Polymeric Materials for the Advancement of Regenerative Medicine

Cited by 30 publications

References 154 publications

Notch signaling and fluid shear stress in regulating osteogenic differentiation

Notch signaling and fluid shear stress in regulating osteogenic differentiation

Notch Signaling and Fluid Shear Stress in Regulating Osteogenic Differentiation

Natural Polymers for the Maintenance of Oral Health: Review of Recent Advances and Perspectives

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