Bioactive polyphenols separated from hemp seed shells ameliorate H₂O₂‐induced oxidative stress injury in human umbilical vein endothelial cells

Abstract

In the present study, we investigated the protection of hemp seed polyphenols on human umbilical vein endothelial cells (HUVEC) from H 2 O 2 -mediated oxidative stress injury. Fractions with different polarities were obtained by separating the hemp seed extract using HPD300 macroporous resinpacked column. The fraction, desorbed by 50% ethanol, is rich in polyphenol (789.51 ± 21.92 mg GAE/g) and has the highest antioxidant activity in vitro. HPLC-QTOF-MS/MS identified the main polyphenol components in hemp seed shells: 4 hydroxycinnamic acid amides and 15 lignanamides. The protective effects of hemp seed polyphenol against oxidative-stress injury in HUVEC cells were evaluated by cell viability, intracellular antioxidant parameters, and cell apoptosis assay. After HUVEC cells were precultured with 50 µg/ml hemp seed polyphenols, the cell viability increased significantly from 53.07 ± 2.46% (model group) to 80.65 ± 1.32% (p < 0.01). In addition, the pretreatment of HUVEC cells with polyphenol could substantially increase their intracellular superoxide dismutase activity and reduce their intracellular reactive oxygen species level, malondialdehyde content, and lactate dehydrogenase leakage index. These findings demonstrate the defensive potential of hemp seed polyphenol in reducing the incidence of cardiovascular disease.

Exploring the neuroprotective activity of a lignanamides-rich extract in human neuroblastoma SH-SY5Y cells under dimethyl sulfoxide-induced stress

“…In agreement with these notions, in our study, we found an increase in NO generation in cells exposed to DMSO, which could inhibit neuronal viability. In accordance with our data, Yuan C. et al demonstrated that DMSO increases ROS production in a dosedependent manner in astrocytes cultures (Yuan et al, 2014) (Xu et al, 2023). SIRT3 and SOD2 are key proteins in the oxidative metabolism (Bause and Haigis, 2013;Flynn and Melov, 2013).…”

“…It is interesting to notice that LnHS administration counteracted DMSO effects in the co-treatment condition, strongly suggesting that LnHS can enhance neuronal survival, at least in part, by reducing of oxidative stress. In line with our hypothesis, Xu P. W. et al demonstrated that pretreatment of HUVEC cells with HS polyphenols substantially decreased ROS levels induced by H 2 O 2 administration ( Xu et al, 2023 ). SIRT3 and SOD2 are key proteins in the oxidative metabolism ( Bause and Haigis, 2013 ; Flynn and Melov, 2013 ).…”

“…Phenylamides and lignanamides constitute a small group of natural compounds with their broader distribution in the plant kingdom ( Nigro et al, 2020 ). These compounds have been found to display interesting and diverse biological activities, including anti-inflammatory activities which suggest the consumption of HS for the prevention of several pathological conditions such as cardiovascular disorders ( Nigro et al, 2020 ; Xu et al, 2023 ). Nevertheless, one of the most crucial features of lignanamides is the ability to preserve the neuronal health, making them promising neuroprotective candidates.…”

The Therapeutic Potential of Hemp Seed Oil in D-Galactose-Induced Aging Rat Model Was Determined through the Combined Assessment of 1H NMR Metabolomics and 16S rRNA Gene Sequencing

“…Hemp seed oil exhibits diverse biological activities. Prior studies have demonstrated its potential in enhancing antioxidant capacity, counteracting aging processes, and improving cognitive function [ 3 , 4 , 5 ]. In the present study, our findings offer valuable insights and a comprehensive understanding of the D-galactose-induced aging model, elucidating the role of hemp seed oil in modulating metabolic networks and gut microbiota.…”

“…Evidence has emerged supporting the beneficial effects of a dietary combination comprising hemp seed and bitter vegetable ( Sonchus oleraceus ) on health, longevity, and cognitive function [ 4 ]. Previous investigations have suggested that hemp seed polyphenols exhibit robust antioxidant activity in vitro and effectively shield human umbilical vein endothelial cells (HUVEC) against oxidative stress damage induced by H 2 O 2 [ 5 ]. However, the precise mechanism behind the anti-aging properties of hemp seed oil remains poorly understood.…”