Bioactive properties and functional constituents of Hypericum androsaemum L.: A focus on the phenolic profile

Abstract

Hypericum androsaemum L. ethanol:water extract acted as a lipid peroxidation inhibitor and free radical scavenger. A marked inhibition of the growth of breast, lung, cervical and hepatocellular human carcinoma cell lines was also observed, whereas no toxicity was shown against non-tumor porcine liver cells (>400μg/mL). The extract was also effective in inhibiting nitric oxide production, as an indicator of the anti-inflammatory potential. The anti-Candida effects varied among different strains of the same species, C. glabrata and C. tropicalis being the most sensible species with an effect directly related with the extract concentrations tested. A significant anti-biofilm formation potential was also observed, namely for C. glabrata and C. tropicalis (biofilm reduction >90%). 5-O-Caffeoylquinic and 3-O-caffeoylquinic acids were the most abundant phenolic compounds identified in the extract, and might be related with the observed bioactive effects. Nevertheless, future studies should be carried out to obtain dose-response curves of the isolated active compounds, in order to perform further pre-clinically testing to quantify the presence of the most active compounds in the extract.

Hibiscus sabdariffa L. as a source of nutrients, bioactive compounds and colouring agents

“…The antioxidant activity (DPPH radical-scavenging activity, reducing power, β-carotene bleaching inhibition and TBARS formation inhibition) was evaluated by colorimetric assays, previously described by Jabeur et al (2016). Trolox, a water-soluble analogue of vitamin E, was used as a positive control.…”

Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties

“…Therefore, there is an increasing interest in studying other Hypericum species, in order to collect scientific data which could indicate them as a potentially new source of biologically active compounds and to verify its traditional usage. Some of them have been studied regarding their chemical composition and various biological activities (antioxidant, antimicrobial, antidepressive, cytotoxic, antiinflammatory activities) [6][7][8][9][10][11].…”

“…The final identification of compounds was performed by comparing their retention times, UV and MS spectra with the appropriate retention times and spectra of standard commercially available compounds or flavonoids previously isolated in our laboratory from the H. richeri [12]. In the analyzed extracts 17 compounds were identified: (1) 3-O-, (2) 4-O-, and (3) 5-O-caffeoylquinic acid, (4) orientin, (5) myricetin 3-Orutinoside, (6) myricetin 3-O-galactoside, (7) myricetin 3-Oglucoside, (8) rutin, (9) hyperoside, (10) 2''-O-acetyl-orientin, (11) isoquercitrin, (12) quercitrin, (13) astilbin, (14) quercetin, (15) I3,II8-biapigenin, (16) pseudohypericin, and (17) hypericin.…”

Stevia rebaudiana Bertoni cultivated in the field and obtained by in vitro micropropagation: A prospective study of the antioxidant potential in different culture and storage conditions

“…cellulose, proteins and lignin) [14]. Tocopherols have been recognized as the most important natural antioxidants in foods, being this effect mainly conferred by their prominent action as free radical scavengers and hydrogen donators [15]; -tocopherol is the isoform that confers the highest biological potential. On the other hand, 5-O-caffeoylquinic acid is the most predominant form of chlorogenic acid present in plants, being its antioxidant potential mainly attributed to its double bond conjugated with the catechol structure of phenyl ring.…”

“…Influence of pH and plant growth regulators on secondary metabolite production and antioxidant activity of Stevia rebaudiana (Bert). Period.Biol., 118,[9][10][11][12][13][14][15][16][17][18][19] Soufi, S., D'Urso G., Pizza C., Rezgui S., Bettaieb T., Montoro P., 2016. Steviol glycosides targeted analysis in leaves of Stevia rebaudiana (Bertoni) from plants cultivated under chilling stress conditions.…”