Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface

Deng, Chengbin; Zhu, Hai; Li, Jiayi; Feng, Chun; Yao, Qingqiang; Wang, Liming; Chang, Jiang; Wu, Chengtie

doi:10.7150/thno.23674

Cited by 107 publications

(86 citation statements)

References 59 publications

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“…In this context, tissue engineering has emerged as a potential alternative treatment that could provide biological tissue substitutes for replacing the damaged ones, using scaffolds and cells. However, although many efforts have been made, very few tissue engineering techniques have been translated into clinical practice, and the ideal scaffold for engineering bone and cartilage substitutes has not yet been developed [1,[4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Piñeiro-Ramil

Sanjurjo‐Rodríguez

Castro-Viñuelas

et al. 2019

IJMS

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The unavailability of sufficient numbers of human primary cells is a major roadblock for in vitro repair of bone and/or cartilage, and for performing disease modelling experiments. Immortalized mesenchymal stromal cells (iMSCs) may be employed as a research tool for avoiding these problems. The purpose of this review was to revise the available literature on the characteristics of the iMSC lines, paying special attention to the maintenance of the phenotype of the primary cells from which they were derived, and whether they are effectively useful for in vitro disease modeling and cell therapy purposes. This review was performed by searching on Web of Science, Scopus, and PubMed databases from 1 January 2015 to 30 September 2019. The keywords used were ALL = (mesenchymal AND ("cell line" OR immortal*) AND (cartilage OR chondrogenesis OR bone OR osteogenesis) AND human). Only original research studies in which a human iMSC line was employed for osteogenesis or chondrogenesis experiments were included. After describing the success of the immortalization protocol, we focused on the iMSCs maintenance of the parental phenotype and multipotency. According to the literature revised, it seems that the maintenance of these characteristics is not guaranteed by immortalization, and that careful selection and validation of clones with particular characteristics is necessary for taking advantage of the full potential of iMSC to be employed in bone and cartilage-related research.

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Section: Introductionmentioning

confidence: 99%

“…Not completely immortalized because E6/E7 was sufficient to extend lifespan but not to bypass senescence 4. Probably incompletely immortalized 5 [30,68,69]. employed hMSC-hTERT-derived clones.…”

mentioning

confidence: 99%

Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Piñeiro-Ramil

Sanjurjo‐Rodríguez

Castro-Viñuelas

et al. 2019

IJMS

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“…For the treatment of OA, simultaneously reconstructing cartilage and subchondral bone is necessary. However, it remains a major problem to develop one single scaffold due to the different physiological functions of cartilage and subchondral bones possess …”

Section: Future Researchmentioning

confidence: 99%

Functional role of hedgehog pathway in osteoarthritis

Xiao

Deng

et al. 2019

Cell Biochemistry & Function

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The hedgehog signalling pathway is one of the key regulators of metazoan development, and it plays an important role in the regulation of a variety of developmental and physiological processes. But it is aberrantly activated in many human diseases, including osteoarthritis (OA). In this study, we have reviewed the association of hedgehog signalling pathway in the development and progression of OA and evaluated the efforts to target this pathway for the prevention of OA. Usually in OA, activation of hedgehog induces up-regulation of the expression of hypertrophic markers, including type X collagen, increases production of nitric oxide and prostaglandin E2, several matrix-degrading enzymes including matrix metalloproteinase and a disintegrin and metalloproteinase with thrombospondin motifs in human knee joint cartilage leading to cartilage degeneration, and thus contributes in OA. Targeting hedgehog signalling might be a viable strategy to prevent or treat OA. Chemical inhibitors of hedgehog signalling is promising, but they cause severe side effects. Knockdown of HH gene is not an option for OA treatment in humans because it is not possible to delete HH in larger animals. Efficient knockdown of HH achieved by local delivery of small interfering RNA in future studies utilizing large animal OA models might be a more efficient approach for the prevention of OA. However, it remains a major problem to develop one single scaffold due to the different physiological functions of cartilage and subchondral bones possess. More studies are necessary to identify selective inhibitors for efficiently targeting the hedgehog pathway in clinical conditions.

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“…OCD including lesions or degeneration of cartilage, subchondral bone, and bone-cartilage interfaces are notorious for being unable to heal. In order to repair OCD, the tissue complex of bone, cartilage, and bone-cartilage interfaces must be taken into account for repair and regeneration [5,6]. Yet, those OCD are difficult to treat because the cartilage and the subchondral bone are tissues with different intrinsic healing capacities.…”

Section: Introductionmentioning

confidence: 99%

Animal Models of Osteochondral Defect for Testing Biomaterials

Meng

Ziadlou

Grad

et al. 2020

Biochemistry Research International

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The treatment of osteochondral defects (OCD) remains a great challenge in orthopaedics. Tissue engineering holds a good promise for regeneration of OCD. In the light of tissue engineering, it is critical to establish an appropriate animal model to evaluate the degradability, biocompatibility, and interaction of implanted biomaterials with host bone/cartilage tissues for OCD repair in vivo. Currently, model animals that are commonly deployed to create osteochondral lesions range from rats, rabbits, dogs, pigs, goats, and sheep horses to nonhuman primates. It is essential to understand the advantages and disadvantages of each animal model in terms of the accuracy and effectiveness of the experiment. Therefore, this review aims to introduce the common animal models of OCD for testing biomaterials and to discuss their applications in translational research. In addition, we have reviewed surgical protocols for establishing OCD models and biomaterials that promote osteochondral regeneration. For small animals, the non-load-bearing region such as the groove of femoral condyle is commonly chosen for testing degradation, biocompatibility, and interaction of implanted biomaterials with host tissues. For large animals, closer to clinical application, the load-bearing region (medial femoral condyle) is chosen for testing the durability and healing outcome of biomaterials. This review provides an important reference for selecting a suitable animal model for the development of new strategies for osteochondral regeneration.

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Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface

Cited by 107 publications

References 59 publications

Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Usefulness of Mesenchymal Cell Lines for Bone and Cartilage Regeneration Research

Functional role of hedgehog pathway in osteoarthritis

Animal Models of Osteochondral Defect for Testing Biomaterials

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