Bioactive Secondary Metabolites from Aspergillus fumigatus ON428521 Isolated from Wadi El Rayan, El Fayum Governorate

Abstract

Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 15 F UNGI were isolated from Wadi El Rayan soils, and their secondary metabolites, extracted by petroleum ether, ethyl acetate, and chloroform, were tested for antimicrobial activities. Among the isolates, fungus-F1 extracts exhibited remarkable inhibitory activity against the tested microorganisms; therefore, it was subjected to morphological and molecular identification, and the fungus identified as Aspergillus fumigatus ON428521. With an inhibition zone ranging from 7mm to 21.4mm, ethyl acetate extract exerted the greatest activity. However, the petroleum ether extract only reached 16.5mm, and the chloroform extract demonstrated a lower activity level. Interestingly, activity was higher against tested Gram negative than those of Gram positive bacteria. Furthermore, Escherichia coli, Streptococcus mutans, and Cryptococcus neoformans were treated with ethyl acetate extract to explore possible effects on ultrastructure using transmission electron microscopy. The cell walls of treated cells lost their uniformity, ruptured, and became thicker. Moreover, the antitumor activities were evaluated against hepatocellular carcinoma (HepG2) and prostate carcinoma (PC-3) cell lines using the MTT assay, which revealed a high inhibitory activity with petroleum ether extract (IC 50 = 61.02 ± 1.64 and 90.31± 2.37µg/mL, respectively). Lower inhibition was detected with ethyl acetate extract (IC 50 of 84.42±3.36 and 121.98 ±3.46µg/ mL against HepG2 and PC-3, respectively). In addition, antioxidant activity was evaluated using the DPPH radical scavenging assay, and ethyl acetate extract demonstrated moderate activity with IC 50 = 278.24 ± 8.52µg/mL, followed by petroleum ether (581.07 ± 41.95µg/mL). The phytochemical analysis showed that the ethyl acetate extract is rich in phenolics, followed by flavonoids and alkaloids while lipids are the major component of the petroleum ether extract. GC-MS analysis of petroleum ether extract gives dodecanamine, N, N-dimethyl-, lupeol acetate, amyrin, kojic acid, and oleanenol-acetate as the major components. In conclusion, ethyl acetate and petroleum ether extracts of A. fumigatus F1 exhibited moderate antimicrobial, antioxidant, and antitumor properties.

Green Synthesis and Characterization of Titanium Dioxide Nanoparticles by Aspergillus niger DS22 and Its Potential Application in Medical Fields

“…Naphthyle acetic acid 7.241 7.873 5.701 Antibacterial and antifungal activity (Gowri et al, 2013) and carcinoma of the mouth and to manage such cancer diseases (Abdullah et al, 2021;El-Baba et al, 2021), and has antioxidant effects (Wu & Zhou, 2023). Similar to previous research, the compounds or their derivatives realized in this research, such as Octadecanamide, N-(2-methyl propyl)-N-nitroso,1-Dodecene, Tetradecane, and Cyclodecane, have antibacterial, antioxidant, antitumor, and anti-inflammatory properties (Mokhtar et al, 2023).…”

“…fungi and can generate the most physiologically active secondary metabolites (Mokhtar et al, 2023).compounds produced by Aspergillus species were classified as terpenoids, alkaloids, steroids, lignans, quinones, acetones, and phenols (Abdel-Wahhab et al, 2020). Many biological activities were documented in other investigations utilizing these flavonoid and phenolic compounds.…”

GC-MS Based Characterization, Antibacterial, Antifungal and Anti-Oncogenic Activity of Ethyl Acetate Extract of Aspergillus niger Strain AK-6 Isolated from Rhizospheric Soil

“…The World Data Centre for Micro-organisms (WDCM) reports about 378 species of Aspergillus, of which approximately 180 fungal species are of pharmaceutical and commercial importance [18][19][20]. Due to its diversity, Aspergillus undoubtedly remains one of the most significant contributors to interesting secondary metabolites that exhibit anti-inflammatory, anticancer, antioxidant and antibacterial activities [21]. Sunil et al (2014) reported that Aspergillus oryzae produces a high level of L-Glutaminas and potential anti-oncogenic activity against the MCF-7 cell line of human breast cancer [22].…”

Synthesis, characterization, biological potency, and molecular docking of Co2+, Ni2+ and Cu2+ complexes of a benzoyl isothiocyanate based ligand

“…The prepared H 4 DAP ligand and its complexes with metals were investigated for in vitro cytotoxicity utilizing the MTT, and crystal violet examinations targeting human HepG-2 (acquired from the ATCC American Type Culture Collection) and MDA-MB-231 (acquired from the VACSERA Tissue Culture Unit) cell lines 28 – 30 . Specific steps are in the supplementary data.…”