Bioactivity Assessment of Poly(ɛ‐caprolactone)/Hydroxyapatite Electrospun Fibers for Bone Tissue Engineering Application

Abstract

Electrospinning is useful for fabricating nanofibrous structure with different composition and morphologies. It offers great advantages through its geometrical structure and biomimetic property, which can provide a suitable environmental site for cell growth. The fiber diameter is entangled by the concentration of PCL with some adjustment of parameters during electrospinning process. PCL with lower concentration had bead structure while higher concentration had smooth fiber. The incorporation of nanoparticle hydroxyapatite (nHA) into poly(ɛ-caprolactone) fiber was studied. The fiber diameter of PCL was increased with the addition of nHA. Composition of fiber at lower concentrations of PCL and nHA into the polymer produced fiber with a homogenous distribution of nHA in PCL fiber with less agglomeration. The immersion of PCL/nHA fiber in simulated body fluid (SBF) had bone-like apatite layer on its surface while PCL showed no results. PCL/nHA showed high water uptake and had improved wettability compared to PCL alone, suggesting that PCL/nHA fibers were more hydrophilic than PCL fiber.

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

“…The main objective of this study was to investigate the effect of the incorporation of nHA and TCH on PCL membrane and compare the morphology, wettability and surface roughness. As reported in our previous study (Hassan et al 2014), incorporation of 10% (w/w) nHA in PCL membrane resulted in a membrane with better fiber properties; the present study will focus on morphology, wettability, mechanical analysis, antibacterial ability and drug release of nanofibers composed of pure PCL and 10% (w/w) nHA/PCL membrane.…”

“…1; Table 1). Based on our previous works, 7.5% (w/v) PCL was optimized, since a higher concentration produced an even larger diameter with non-uniform fiber morphology (Hassan et al 2014). The average fiber diameter of PCL fiber was 0.25 ± 0.08 lm.…”

“…Hence, nHA is usually combined with biopolymer and organic material into a composite form to overcome the limitations of its mechanical properties. Many studies have been reported on the fabrication of composite scaffolds using nHA with biocompatible polymers, such as poly-L-lactic acid (PLLA) (Davachi et al 2016), poly-L-glycolic acid (PLGA) (Zheng et al 2013), poly(hydroxybutyrate-co-valerate) (PHBV) (Sultana and Wang 2008), cellulose (Zadegan et al 2011), PCL (Hassan et al 2014), and polyvinyl alcohol (PVA) (Sultana and Zainal 2016).…”

Drug loading, drug release and in vitro degradation of poly(caprolactone) electrospun fibers

“…There are a number of polymers used to fabricate scaffolds. These polymers include poly(lactic acid) (PLA), poly(caprolactone) (PCL) [2,4,7,8], poly(glycolic acid) (PGA), poly(lactide-co-glycolide) (PLGA) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) [9][10][11][12].…”

A nano-sandwich construct built with graphene nanosheets and carbon nanotubes enhances mechanical properties of hydroxyapatite–polyetheretherketone scaffolds

“…Previous studies had shown that HAP could facilitate the formation of apatite layers between the implant material and living bone tissue. 50,51 As we know, cytocompatibility is an important prerequisite of scaffolds intended for bone tissue engineering application. The S5 scaffold was selected to evaluate the cytocompatibility because of its better mechanical properties and good apatite-forming ability.…”