Bioactivity guided isolation of anticancer constituents from leaves of Alnus sieboldiana (Betulaceae)

Ludwiczuk, Agnieszka; Saha, Achinto; Kuzuhara, Takashi; Asakawa, Y.

doi:10.1016/j.phymed.2010.10.005

Cited by 40 publications

(29 citation statements)

References 52 publications

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“…Previous studies have reported that Alnus plants possess a variety of terpenoids, flavonoids, polyphenols, steroids, and tannins, besides. Numerous bioactivities of genus Alnus were reported, including hepato-protective, antioxidant, and antitumor activities [26][27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Activation of JNK and p38 in MCF-7 Cells and the In Vitro Anticancer Activity of Alnus hirsuta Extract

Ryu

Sung

et al. 2020

Molecules

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JNK and p38 are important mitogen-activated protein kinases (MAPKs) that respond to stress stimuli. The stress-activated MAPKs associated with apoptotic cell death play vital roles in mammalian cells. Alnus hirsuta, which contains abundant diarylheptanoids derivatives, is a valuable medicinal plant. The CHCl3 extract (AHC) containing platyphyllenone (1) and platyphyllone (3) as main compounds showed in vitro anticancer effects. We report the biological activities of A. hirsuta extract associated with the regulation of apoptosis and JNK and p38 in MCF-7 breast cancer cells. Levels of phospho-JNK and phospho-p38 by AHC treatment were evaluated by enzyme-linked immunosorbent assay (ELISA). ROS production, apoptotic effect, and DNA contents of the cells were measured by flow cytometry. The two diarylheptanoids 1 and 3 and the AHC extract exhibited cytotoxic effects on MCF-7 cells in MTT assay, with IC50 values of 18.1, 46.9, 260.0 μg/mL, respectively. AHC induced ROS generation and elevated the endogenous levels of phospho-JNK and phospho-p38. AHC resulted in apoptosis and cell cycle arrest. We suggest that the antitumor effect of A. hirsuta extract is achieved by apoptosis promotion and cell cycle arrest mediated by the activation of JNK and p38 signaling pathway via ROS generation.

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Section: Introductionmentioning

confidence: 99%

Activation of JNK and p38 in MCF-7 Cells and the In Vitro Anticancer Activity of Alnus hirsuta Extract

Ryu

Sung

et al. 2020

Molecules

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“…Extraction process were followed based on Pan et al; Ludwiczuk et al and Forgo et al [15][16][17] methods with some modification. The dried and ground active cocoa plant part (1 kg) of Theobroma cacao was extracted with methanol (10 L) by soaking in room temperature for 3 days.…”

Section: Plant Extraction and Fractionation Preparationmentioning

confidence: 99%

Anticancer Agents from Non-Edible Parts of Theobroma cacao

Zainal¹

2014

Nat Prod Chem Res 2014

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“…Regarding the hydrophilic characteristic of resveratrol, its low bioavailability and rapid clearance from the circulation, attention has been focused on its more lipophilic derivatives [18] , such as pinosylvin (trans-3,5-dihydroxystilbene, Figure 1). This naturally occurring resveratrol analogue is formed constitutively and after UV irradiation or microbial attack in the wood, needles and leaves of Pinus and Alnus species [19] . The majority of the data available characterize antifungal, antibacterial and anticancer activities of pinosylvin [20,21] ; however, little is known about its antioxidant and anti-inflammatory effects [22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

The natural stilbenoid pinosylvin and activated neutrophils: effects on oxidative burst, protein kinase C, apoptosis and efficiency in adjuvant arthritis

et al. 2012

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Aim: To investigate the effects of the naturally occurring stilbenoid pinosylvin on neutrophil activity in vitro and in experimental arthritis, and to examine whether protein kinase C (PKC) activation served as an assumed target of pinosylvin action. Methods: Fresh human blood neutrophils were isolated. The oxidative burst of neutrophils was evaluated on the basis of enhanced chemiluminescence. Neutrophil viability was evaluated with flow cytometry, and PKC phosphorylation was assessed by Western blotting analysis. Adjuvant arthritis was induced in Lewis rats with heat-killed Mycobacterium butyricum, and the animals were administered with pinosylvin (30 mg/kg, po) daily for 21 d after arthritis induction. Results: In isolated human neutrophils, pinosylvin (10 and 100 µmol/L) significantly decreased the formation of oxidants, both extraand intracellularly, and effectively inhibited PKC activation stimulated by phorbol myristate acetate (0.05 µmol/L). The inhibition was not due to neutrophil damage or increased apoptosis. In arthritic rats, the number of neutrophils in blood was dramatically increased, and whole blood chemiluminescence (spontaneous and PMA-stimulated) was markedly enhanced. Pinosylvin administration decreased the number of neutrophils (from 69 671±5588/µL to 51 293±3947/µL, P=0.0198) and significantly reduced the amount of reactive oxygen species in blood. Conclusion: Pinosylvin is an effective inhibitor of neutrophil activity, and is potentially useful as a complementary medicine in states associated with persistent inflammation.

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Bioactivity guided isolation of anticancer constituents from leaves of Alnus sieboldiana (Betulaceae)

Cited by 40 publications

References 52 publications

Activation of JNK and p38 in MCF-7 Cells and the In Vitro Anticancer Activity of Alnus hirsuta Extract

Activation of JNK and p38 in MCF-7 Cells and the In Vitro Anticancer Activity of Alnus hirsuta Extract

Anticancer Agents from Non-Edible Parts of Theobroma cacao

The natural stilbenoid pinosylvin and activated neutrophils: effects on oxidative burst, protein kinase C, apoptosis and efficiency in adjuvant arthritis

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