Bioactivity-Guided Isolation of Anticancer Agents from &lt;i&gt;Bauhinia Kockiana&lt;/i&gt; Korth.

Chew, Yik Ling; Lim, Yau Yan; Stanslas, Johnson; Ee, Gwendoline Cheng Lian; Goh, Joo Kheng

doi:10.4314/ajtcam.v11i3.40

Cited by 13 publications

(9 citation statements)

References 24 publications

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“…Another study investigated hesperidin impact on the proliferation of MCF-7 human breast cancer cells, and prostate cancer cells [31]. On the other hand, pyrogallol, a major phenolic compound identified from V. faba exhibited a moderate effect against prostate cancer cell in a previous study Chew et al [32] Catechol which is another significant phenolic identified from P. sativum apparently turned out to have a sensible effect on two breast cancer cell lines (MCF-7 and MDA-MB-231 [33]. q-Hydroxy benzoic acid, a phenolic acid present in P. sativum and V. faba with great amounts was previously proven to have strong cytotoxic activity on both colon (HCT116) and liver (HEPG2) carcinoma cell line [34].…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

El-Feky

Elbatanony

Mounier

2018

Egyptian Journal of Basic and Applied Sciences

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For a long time, plant secondary metabolites have been strongly examined for their antitumor and cytotoxic impacts. These days, there is another pattern of making utilization of the waste products of plants because of their extravagance of numerous phytochemical components and adequacy on human wellbeing. This research work is handling the effect of diversity of lipoidal and phenolic compounds found in the peels of two common edible plants in the Middle East; Pisum sativum and Vicia faba L. for their assesment as anticancer agents. The GC/MS of the n-hexane extract of both plant peels led to identification of twenty compounds (82.99%) and seventeen compounds (85.97%) of the total lipoidal contents from P. sativum and V. faba, respectively. While the HPLC analysis of the ethyl acetate fraction of the two plant peels resulted in recognition of 17 flavonoids and 18 phenolics from P. sativum and 16 flavonoids and 17 phenolics from V. faba. Moreover, four flavonoidal compounds were isolated to our knowledge for the first time from the peels and tested separately against different human cancer cell lines and the mode of action of the most potent compound has been determined. P. sativum ethyl acetate fraction possessed the highest scavenging activity (31.2%) as well as the most cytotoxic effect on breast carcinoma cell line. Apigenin proved to be the most potent tested compound on (MCF-7) and has no cytotoxic effect on normal human skin cell lines.

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Section: Discussionmentioning

confidence: 99%

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

El-Feky

Elbatanony

Mounier

2018

Egyptian Journal of Basic and Applied Sciences

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“…Voucher specimen (MUM-LEGUM-001) was deposited in the herbarium of School of Science, Monash University Malaysia. The extraction of flowers was performed as according to Chew et al [ 13 ] with slight modifications. Fresh flowers of B. kockiana (1.7 kg), were collected and washed with distilled water, then freeze-dried using a freeze dryer.…”

Section: Methodsmentioning

confidence: 99%

“…Bauhinia species are from Caesalpinioideae subfamily of Leguminosae. Bauhinia kockiana is a plant originates from Peninsular Malaysia but now it can also be found in the tropical forests of Thailand and Sumatra [ 13 ]. It is usually grown in the garden as ornamental plant because of its bright orange-red colour (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Anti-methicillin-resistance Staphylococcus aureus (MRSA) compounds from Bauhinia kockiana Korth. And their mechanism of antibacterial activity

Chew

Mahadi

Wong

et al. 2018

BMC Complement Altern Med

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BackgroundBauhinia kockiana originates from Peninsular Malaysia and it is grown as a garden ornamental plant. Our previous study reported that this plant exhibited fairly strong antioxidant and antimicrobial activities. This paper focused on the assessment of the antibacterial activity of B. kockiana towards methicillin-resistance Staphylococcus aureus (MRSA), to purify and to identify the antibacterial compounds, and to determine the mechanism of antibacterial activity.MethodsAntibacterial activity of B. kockiana flower was evaluated qualitatively and quantitatively using disc diffusion assay and microbroth dilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of extracts were examined. Phytochemical analysis was performed to determine the classes of phytochemicals in the extracts. Bioactivity guided isolation was employed to purify the antibacterial agents and identified via various spectroscopy methods. Scanning electron microscopy (SEM) technique was used to evaluate the antibacterial mechanism of extract and compounds isolated.ResultsB. kockiana flower was found to exhibit fairly strong antibacterial activity towards both strains of MRSA bacteria used, MIC varies from 62.5–250 μg/mL. Tannins and flavonoids have been detected in the phytochemical analysis. Gallic acid and its ester derivatives purified from ethyl acetate extract could inhibit MRSA at 250–500 μg/mL. SEM revealed that the cells have undergone plasmolysis upon treatment with the extract and compounds.ConclusionTannins and polyphenols are the antibacterial components towards MRSA in B. kockiana. Massive leakage of the cell content observed in treated cells showed that the phytochemicals have changed the properties of the cell membranes. Amphiphilic nature of the compounds exhibited the antibacterial activity towards MRSA via three stages: (1) cell membrane attachment; (2) cell membrane fluidity modification; and (3) cell membrane structure disruption.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2137-5) contains supplementary material, which is available to authorized users.

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“…In addition, herbal medicines can induce cell death by apoptosis, acting on initiation and tumor resistance [5]. In this context, species of the Bauhinia genus have shown activities on tumor progression stages [6][7][8]. Bauhinia variegata L. is a species of Asian origin, disseminated in the Brazilian territory, along with natural vegetation and in urban areas [9].…”

Section: Introductionmentioning

confidence: 99%

ID7 Isolated from Bauhinia variegata Stem Inhibits Tumor Progression and Metastatic Mechanisms of Triple Negative Breast Cancer in Vivo

Santos¹,

Gomes²,

Oliveira³

et al. 2019

JPP

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Background: The breast cancer has been the most common form of cancer among women. The triple negative subtype represents 20% of all breast cancer cases in the world and is standing out by affecting young women and being aggressive. The main cause of death of patients with cancer is due to metastasis, which can reach the liver and lungs. Objective: The activities of ID7 fraction of the stems of Bauhinia variegata L. on breast cancer, lung metastasis and liver inflammatory process were evaluated. Method: ID7 was characterized by mass-spectrometry. The viability of murine mammary cells (4T1) treated with ID7 was assessed by MTT, trypan blue and fluorescence assay and viability of BT-20, MDA-MB-231 and MCF-7 human breast cancer tumor lines by MTS. The cell migration, invasion using matrigel and adhesion were performed. The expression of cell death proteins was quanitified by western blot and the gelatinases by zimogram. The ID7 activity of the tumor (4T1) and metastatic progession in vivo was evaluated. Results: ID7 reduced the 4T1 and MDA-MB-231viability and increased the late apoptosis, inhibited the 4T1 migration and invasion, increased the 4T1 adhesion and decreased the secreted active gelatinases. ID7 also increased the expression of PARP, caspase-7 and caspase-8, RIP and TNF-R1. In vivo, the ID7 decreased the volume and weight of the tumors and decreased lung metastasis and inflammation in the liver. The characterization showed mainly the presence of oleic acid, myricetin, quercetin and kaempferol in ID7. Conclusion: Thus, it was found that ID7 fraction exhibits selective antitumor and on the mechanisms of breast cancer metastasis activity, preventing lung metastasis and inflammation in the liver. It is suggested that fatty acids and flavonoids are correlated with such activities.

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Bioactivity-Guided Isolation of Anticancer Agents from <i>Bauhinia Kockiana</i> Korth.

Cited by 13 publications

References 24 publications

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

Anti-methicillin-resistance Staphylococcus aureus (MRSA) compounds from Bauhinia kockiana Korth. And their mechanism of antibacterial activity

ID7 Isolated from Bauhinia variegata Stem Inhibits Tumor Progression and Metastatic Mechanisms of Triple Negative Breast Cancer in Vivo

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