Bioactivity guided isolation of antipsychotic constituents from the leaves of Rauwolfia tetraphylla L.

Thermodynamics of the solubility of reserpine in {{2-(2-ethoxyethoxy)ethanol + water}} mixed solvent systems at different temperatures

“…It is an active phytoconstituent of Rauwolfia serpentia which is used as an antihypertensive and antipsychotic drug [3][4][5]. It has been reported as a poorly watersoluble compound in water and most of the organic solvents [6].…”

Bioactivity-guided isolation of antiplasmodial constituents from Conyza sumatrensis (Retz.) E.H. Walker

“…The shade-dried leaves (250 g) were coarsely powdered and extracted thrice with methanol (3 × 1.5 L) [9]. The combined MeOH extract was concentrated under vacuum at 40°C, which yielded 30 g of MeOH extract.…”

“…Gradient elution of the column was carried out with mixtures of n-hexane, CHCl 3 and MeOH in increasing polarity [9]. A total of 80 fractions of 50 mL each were collected and pooled on the basis of their TLC (SiO 2 ) profile after spraying with vanillin sulfuric acid and heating the plate for 5 min at 100°C.…”

A validated and densitometric HPTLC method for the simultaneous quantification of reserpine and ajmalicine in Rauvolfia serpentina and Rauvolfia tetraphylla

“…Moreover, R. serpentina has been reported for pharmacological properties such as antbacterial, antiinflammatory and cytotoxicity (Dey and De, 2010). R. tetraphylla has also been reported to possess antipsychotic (Gupta et al, 2012a), antibacterial activity and anti-inflammatory (Ganga Rao et al, 2012) Earlier Rauvolfia alkaloids were analyzed by quantitative thin layer chromatography (Habib and Court, 1971;Nguyen and Nikolova, 1989). HPLC and TLC identification, estimation and separation of indole alkaloid from R. serpentina and R. vomitoria was also achieved (Cieri, 1983;Klyushnichenko et al, 1994).…”