Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima

Abstract

Identification of alternate bioactive compounds isolated from microbial endophytes from plants may lead to better solution against antibiotic resistance. Main objective of this research is to determine the bioactivity of antibacterial compounds produced by endophytic actinomycetes, which was isolated from stem bark of Neesia altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds was assayed using Thin Layer Chromatography (TLC) by employing bioautography. Bioactivity was assayed using crude extract against five microorganisms using agar diffusion methods. Tetracycline was used as a positive control. The result of antibacterial activity from endophytic actinomycetes isolate revealed that the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria Bacillus cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm. Each of TLC fraction was able to inhibit growth of B. cereus at a minimum concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of that compounds are narrow spectrum. The bioactive compound isolated from endophytic actinomycetes may have other implications such as anticancer, antifungal, and antioxidant.

Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus

“…Secondary metabolite compounds such as antioxidants and antibacteria are produced by bacteria in the stationary phase. According to Pratiwi et al (2018), secondary metabolites are products resulting from the secondary metabolism of microorganisms. Secondary metabolite products are required for microorganisms as emergency nutrients to survive in the stationer phase.…”

Isolation and structure elucidation of phenazine derivative from Streptomyces sp. strain UICC B-92 isolated from Neesia altissima (Malvaceae)

“…Cells of actinomycetes were removed from fermentation broth by filtration and the culture filtrate was extracted by ethyl acetate. Purification of crude bioactive compound was conducted according to the method described by Pratiwi et al (23) in the silica gel chromatography column (22 × 5 cm, Silica gel 60, Merck) and eluted with gradient solvent system consisting of ethyl acetate + hexane. Elutions collected during column chromatography were concentrated and tested for their antimicrobial activity against B. cereus and S. aureus to screen bioactive fractions.…”

A novel bioactive molecule 5-β-Hydroxyltridecanyl benzoate identified in Pedalium Murex L. (Gokhru) fruit extracts showed antifungal activity against Sclerotinia sclerotiorum

“…Hence, there is a high demand for novel antifungals belonging to a wide range of structural classes, selectively acting on new targets with fewer side effects (Abad et al 2007). According to current estimates, about 10 to 20% of staple foods and cash crops are destroyed by plant pathogens (Pratiwi et al 2018). Currently, there is little evidence on the antimicrobial properties of the medicinal plants under investigation against phytopathogen fungi.…”