Bioactivity of Ethyl Acetate Fraction from the Leaves of “Sukun” (Artocarpus Altilis (Parkinson) Fosberg) in Preventing Atherosclerosis

Mozef, Tjandrawati; Risdian, Chandra; Sukandar, Elin Yulinah; Soemardji, Andreanus A.

doi:10.1016/j.proche.2015.12.037

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…In previous studies performed in rats treated with a fraction of A. altilis ethyl acetate and in others fed fruit-based diets of A. altilis , the blood glucose levels were reduced ( 50 , 60 ), whereas these levels are similar in mice and rats treated with extracts from M. citrifolia fruit ( 51 , 52 ).…”

Section: Discussionmentioning

confidence: 86%

Modulation of Gut Microbiota, and Morphometry, Blood Profiles and performance of Broiler Chickens Supplemented with Piper aduncum, Morinda citrifolia, and Artocarpus altilis leaves Ethanolic Extracts

Paredes-López,

Robles-Huaynate,

Soto-Vásquez

et al. 2024

Front. Vet. Sci.

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Bioactive plants such as P. aduncum, M. citrifolia, and A. altilis might improve intestinal health as an alternative to antibiotic growth promoters. The objective of this study was to determine the effect of the ethanolic extracts (EEs) of these plants on the intestinal health of broiler chickens. Cobb 500 chickens (n = 352) were distributed into eight treatments with four replicates and 11 chickens each. T1 received a base diet, and T2 received a base diet with 0.005% zinc bacitracin. T3, T5, and T7 were supplemented with 0.005% of P. aduncum, M. citrifolia, and A. altilis EE in the diet while T4, T6, and T8 with 0.01% of the extract. The EEs were supplemented with drinking water from 1 to 26 days of age. The following parameters were evaluated: hematological profiles at 28 days of age, blood metabolites profiles at 14, 21, and 28 days; Escherichia coli, Staphylococcus aureus, and Lactobacillus sp. abundance in the ileum mucosa and content at 21 and 28 days, and histomorphometry of the duodenum, jejunum, and ileum mucosa at 14, 21, and 28 d. Final weight (FW), weight gain (WG), feed intake (FI), and feed conversion rate (FCR) were evaluated at seven, 21, and 33 days of age. M. citrifolia and A. altilis EE at 0.01% increased blood glucose levels at 21 and 28 days of age, respectively, and P. aduncum and M. citrifolia EE at 0.01% increased triglycerides at 28 days of age; in addition, this EE did not have any effect on the AST and ALT profiles. The depths of the Lieberkühn crypts and the villi length to the crypt’s depth ratio increased with age on supplementation with 0.01% M. citrifolia and A. altilis EE at 21 days of age (p < 0.05). In addition, the depth of the crypts increased at 28 days of age (p < 0.05) in chickens supplemented with 0.01% A. altilis EE. The 0.01% M. citrifolia EE in diet decreased in the Staphylococcus aureus population in the ileal microbiota (p < 0.05). The FW and WG during the fattening and in the three stages overall increased, and the FCR decreased; however, the FI and the carcass yield did not change in the broiler chickens supplemented with 0.01% M. citrifolia EE (p < 0.05). Conclusively, the M. citrifolia EE at 0.01% of the diet improved intestinal health and thus the performance indices of the broiler chickens and did not have a detrimental effect on any of the parameters evaluated, so it is postulated as a potential alternative to AGP in poultry.

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Section: Discussionmentioning

confidence: 86%

Modulation of Gut Microbiota, and Morphometry, Blood Profiles and performance of Broiler Chickens Supplemented with Piper aduncum, Morinda citrifolia, and Artocarpus altilis leaves Ethanolic Extracts

Paredes-López,

Robles-Huaynate,

Soto-Vásquez

et al. 2024

Front. Vet. Sci.

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“…Tiga senyawa flavonoid dari akar sukun yaitu dihydroartomunoxanthone, artochamins B dan artocommunol CC dilaporkan dapat menghambat agregasi platelet pada platelet rich plasma (PRP) darah manusia yang diinduksi adrenalin (Weng et al, 2006). Selain aktivitas antiplatelet, fraksi etil asetat daun sukun menurunkan kadar total kolesterol serum dan mencegah akumulasi lemak pada aorta tikus, sehingga berkontribusi dalam pengobatan penyakit kardiovaskuler (Mozef et al, 2011(Mozef et al, , 2015.…”

Section: Pendahuluanunclassified

STUDI AKTIVITAS ANTIPLATELET DAN ANTITROMBOSIS EKSTRAK AIR DAUN SUKUN (Artocarpus altilis (Park.) Fosberg)

Hastuti

Nurrochmad

Puspitasari

et al. 2021

J. Tumbuhan Obat Indonesia

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ABSTRACT The mature breadfruit leaves (Artocarpus altilis) infusion has been traditionally used by Indonesian folks for curing heart diseases and stroke. The key mechanisms underlying these diseases are platelet aggregation and thrombosis. There is no evidence about the efficacy of the water extract of A. altilis leaves (EADS) against platelet aggregation and thrombosis, in order to provide scientific evidence regarding its use by the community. This study aimed to investigate the antiplatelet and antithrombotic activities of EADS. Ticagrelor, an antiplatelet drug agonist of P2Y12 receptor was used as a positive control. The antiplatelet activity of EADS was assessed in vitro by Light Transmittance Aggregometry (LTA) method using human platelet induced by Adenosine Diphosphate (ADP); whereas the antithrombotic activity was evaluated in vivo using Acute Pulmonary Thromboembolism (APT) method in male adult Swiss mice induced by the mixture of epinephrine (0.7 mg/kg bw) and collagen (11 mg/kg bw). The number and the onset of dead and paralysis mice were observed; and the number of thrombus was calculated under the microscope. We found that EADS demonstrated a weak antiplatelet activity (IC50>1000 µg/mL). Based on the number and the onset of dead and/or paralysis, as well as the number of thrombus, we found that EADS failed to exhibit antithrombotic activity at the doses of 200, 300, and 400 mg/kg bw. TLC analysis showed that EADS did not contain 2-geranyl-2,3,4,4’-tetrahydroxydihydrochalcone (GTDC), the antiplatelet compound in the ethanolic extract of A. altilis leaves (EEDS) in our previous research. Keywords: Artocarpus altilis, platelet aggregation, antithrombotic, Light Transmittance Aggregometry, Acute Pulmonary Thromboembolism ABSTRAK Rebusan daun sukun yang sudah tua (Artocarpus altilis) secara tradisional digunakan oleh masyarakat Indonesia untuk mengobati penyakit jantung dan stroke. Agregasi platelet dan trombosis merupakan faktor penting pada patofisiologi kedua penyakit tersebut. Penelitian aktivitas antiplatelet dan antitrombosis dari Ekstrak Air Daun Sukun (EADS) belum pernah dilaporkan. Penelitian ini bertujuan untuk menguji aktivitas antiplatelet dan antitrombosis dari EADS guna memberikan bukti ilmiah terkait pemanfaatannya oleh masyarakat. Ticagrelor, obat antiplatelet yang merupakan agonis dari reseptor P2Y12 digunakan sebagai kontrol positif. Uji aktivitas antiplatelet dilakukan secara in vitro menggunakan metode Light Transmittance Aggregometry (LTA) dengan platelet yang diambil dari darah manusia dan digunakan induktor agregasi platelet berupa Adenosin Difosfat (ADP, 10µM). Parameter yang diamati adalah persen penghambatan agregasi platelet. Uji aktivitas antitrombosis dilakukan secara in vivo menggunakan metode Acute Pulmonary Thromboembolism (APT) pada mencit jantan dewasa galur Swiss dengan induktor trombosis berupa campuran epinefrin (0,7 mg/kgBB) dan kolagen (11 mg/kgBB). Parameter yang diamati adalah jumlah dan onset kematian, paralisis, serta jumlah trombus berdasarkan analisis histopatologi. Hasil uji menunjukkan bahwa EADS memiliki aktivitas antiplatelet yang lemah (IC50>1000 µg/mL). EADS tidak memiliki aktivitas antitrombosis yang terlihat dari ketidakmampuan dalam melindungi mencit dari paralisis dan/atau kematian serta tidak adanya penurunan jumlah trombus yang bermakna pada mencit yang diberi perlakuan ekstrak dibandingkan kelompok kontrol pelarut. Analisis KLT menunjukkan bahwa EADS tidak mengandung senyawa aktif antiplatelet 2-geranyl-2,3,4,4’-tetrahydroxydihydrochalcone (GTDC) yang ada dalam ekstrak etanol daun sukun (EEDS) pada penelitian sebelumnya. Kata Kunci: Artocarpus altilis, agregasi platelet, antitrombosis, Light Transmittance Aggregometry, Acute Pulmonary Thromboembolism

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“…Studies with extracts, fractions and isolated components of the leaves of the two species demonstrate cardio-protective functions (Nwokocha et al 2017), dendrite elongation inhibition (rao et al 2013), prevent atherosclerosis (Mozef et al 2015) and have antiviral (Hafid et al 2017), hypoglycemic and hypolipidemic effects (Chandrika et al 2006, Chackrewarthy et al 2010.…”

Section: Introductionmentioning

confidence: 99%

Comparative anatomy and histochemistry of the leaf blade of two species of Artocarpus

Sá

Cadena

Padilha

et al. 2019

An. Acad. Bras. Ciênc.

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Bioactivity of Ethyl Acetate Fraction from the Leaves of “Sukun” (Artocarpus Altilis (Parkinson) Fosberg) in Preventing Atherosclerosis

Cited by 5 publications

References 23 publications

Modulation of Gut Microbiota, and Morphometry, Blood Profiles and performance of Broiler Chickens Supplemented with Piper aduncum, Morinda citrifolia, and Artocarpus altilis leaves Ethanolic Extracts

Modulation of Gut Microbiota, and Morphometry, Blood Profiles and performance of Broiler Chickens Supplemented with Piper aduncum, Morinda citrifolia, and Artocarpus altilis leaves Ethanolic Extracts

STUDI AKTIVITAS ANTIPLATELET DAN ANTITROMBOSIS EKSTRAK AIR DAUN SUKUN (Artocarpus altilis (Park.) Fosberg)

Comparative anatomy and histochemistry of the leaf blade of two species of Artocarpus

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