Bioactivity of fluorophenyl thiourea derivatives: Antioxidant efficacy and inhibition of key diabetes‐related enzymes

Abstract

Thiourea structures, known for their wide‐ranging bioactivity, have significant potential in diabetes management. In this study, it was aimed to examine the antioxidant capacities of fluorophenyl thiourea derivative compounds and their inhibition studies on α‐amylase and α‐glycosidase enzyme activity. Antioxidant capacity was determined using Fe3+‐Fe+2, FRAP, and Cu2+‐Cu+ reducing analyses, DPPH· and ABTS·+ scavenging experiments. It was observed that fluorophenyl thiourea derivative compounds exhibited quite high antioxidant activity compared to standard antioxidants such as BHA, BHT, trolox, α‐tocopherol, and ascorbic acid. Additionally, this study investigated the inhibitory effects of the analysis molecules on α‐glycosidase and α‐amylase, which are enzymes associated with diabetes. Among these derivative molecules, 4‐fluorophenyl showed the highest inhibition on α‐amylase (IC50: 53.307 nM) and α‐glycosidase (IC50: 24.928 nM). These results highlight the potential of thiourea derivatives in enzyme inhibition and antioxidant therapy, making them promising candidates for diabetes management.