Bioactivity of Hydnora africana on selected bacterial pathogens: Preliminary phytochemical screening

Nethathe, B B; Ndip, Roland N.

doi:10.5897/ajmr11.566

Cited by 26 publications

(15 citation statements)

References 38 publications

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“…These organisms are human pathogens and are leading causes of hospital and acquired infections. They were selected based on their prevalence and increasing trend of resistance to antibiotics [ 34 , 35 , 36 ].…”

Section: Methodsmentioning

confidence: 99%

Identification and Antibacterial Evaluation of Bioactive Compounds from Garcinia kola (Heckel) Seeds

Seanego

Ndip

2012

Molecules

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We assessed the bioactivity of G. kola seeds on Streptococcus pyogenes, Staphylococcus aureus, Plesiomonas shigelloides and Salmonella typhimurium. The crude ethyl acetate, ethanol, methanol, acetone and aqueous extracts were screened by the agar-well diffusion method and their activities were further determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays. The extracts were fractionated by Thin Layer Chromatography (TLC). Bioautography was used to assess the activity of the possible classes of compounds present in the more active extracts. Column chromatography was used to purify the active compounds from the mixture, while GC-MS was used to identify the phytocomponents of the fractions. The inhibition zone diameters of the extracts ranged from 0–24 ± 1.1 mm, while MIC and MBC values ranged between 0.04–1.25 mg/mL and 0.081–2.5 mg/mL, respectively. The chloroform/ethyl acetate/formic acid (CEF) solvent system separated more active compounds. The MIC of the fractions ranged between 0.0006–2.5 mg/mL. CEF 3 (F3), CEF 11 (F11) and CEF 12 (F12) revealed the presence of high levels of linoleic acid, 1,2-benzenedicarboxylic acid and 2,3-dihydro-3,5-dihydroxy-6-methyl ester, respectively. The results obtained from this study justify the use of this plant in traditional medicine and provide leads which could be further exploited for the development of new and potent antimicrobials.

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Section: Methodsmentioning

confidence: 99%

Identification and Antibacterial Evaluation of Bioactive Compounds from Garcinia kola (Heckel) Seeds

Seanego

Ndip

2012

Molecules

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“…-CDOCKER interaction signifies the energy of the non bonded interaction that exists between the protein and the ligand. The criteria for best interaction was chosen based on a) high positive value of-CDOCKER energy and b) small difference between -CDOCKER energy and-CDOCKER interaction energy [5,7]. Table 1 shows that acetaldehyde dehydrogenase-Flavone interaction has the highest positive value of -CDOCKER energy 16.2476 between-CDOCKER interaction energy and-CDOCKER energy.…”

Section: Resultsmentioning

confidence: 99%

Hydnora africana Derived Phytochemicals against Acetaldehyde Dehydrogenase of Aeromonas hydrophila Causing Septicemia

Tripathy

Adhikari

Pandey

et al. 2020

JPRI

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For drug discovery it takes approximately 6 years to expose in the market and for commercial uses. There are different procedure to get success in the drug discovery like preliminary phytochemical analysis, structural elucidation of the bioactive compound, preclinical test and clinical test etc. So to optimize the time for invention of new drug molecule, computer aided drug designing and molecular docking analysis is being used as one of the highly effective methodology. The phytochemical extraction of Hydnora africana plant was reported to inhibit the growth of Aeromonas hydrophila which cause Septicemia. “Biovia Discovery Studio” molecular docking methods give us opportunity to identify the effective molecule against the microbes. “High positive values of -CDOCKER energy and -CDOCKER interaction energy” recommended that flavone can effectively deactivate the acetaldehyde dehydrogenase enzyme thereby interrupting the life cycle of the organism.

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“…Tannins and phenols were absent in G. barbadense; also G. hirsutum had a significantly higher (p<0.05) quantity of flavonoids than G. barbadense. Phytochemical compounds such as alkaloids, saponins, tannins, phenols and flavonoids have been known to be biologically active and thus partially responsible for the antimicrobial activities of plants, hence their use in traditional medicine (Rao et al, 2010;Ajibesin et al, 2011;Nethathe and Ndip, 2011;Aderiye et al, 2014).…”

Section: Antimicrobial Testmentioning

confidence: 99%

<i>Gossypium hirsutum</i> L. and <i>Gossypium barbadense</i> L.: differences in phytochemical contents, antioxidant and antimicrobial properties.

Ade-Ademilua¹,

Okpoma²

2018

Ife Journal of Science

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Gossypium L. generally called cotton plants are used indiscriminately in traditional medicine without cognisance to the fact that there are different cotton species and the likelihood of differences in phytochemical content and ultimately, their medicinal capabilities. This study reports the differences in the phytochemical contents, antioxidant and antimicrobial properties of extracts of leaves of two cotton species, Gossypium hirsutum L. and Gossypium barbadense L. Leaf samples from mature G. hirsutum and G. barbadense plants were collected, shadedried and powdered. Phytochemical contents were quantified while antioxidant activity was tested through 1,1diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and reducing power. Aqueous, decocted aqueous and ethanol leaf extracts of G. hirsutum and G. barbadense were tested against Esherichia coli (known to cause gastrointestinal infections and urinary tract infections), Staphylococcus aureus (known to cause skin infection), Klebsiella pneumoniea and Pseudomonas aeruginosa (known to cause bronchial infections and pneumonia). The results showed that tannins and phenols were present in leaves of G. hirsutum but absent in G. barbadense. Leaves of G. hirsutum contained significantly higher (at p<0.05) amount of total glycosides (156.44 ± 2.05 mg /100g) and flavonoid (120.85 ± 0.32 mg /100g, twice as much) than G. barbadense. Gossypium hirsutum showed higher DPPH free radical scavenging activity and reducing power than G. barbadense. Gossypium hirsutum showed higher zones of inhibition (mm) in all organisms than G. barbadense. It can therefore be concluded that G. hirsutum leaves have higher phytochemical contents as well as antioxidant and antimicrobial activities than G. barbadense. Gossypium hirsutum is therefore recommended for use as against G. barbadense where the choice is available.

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Bioactivity of Hydnora africana on selected bacterial pathogens: Preliminary phytochemical screening

Cited by 26 publications

References 38 publications

Identification and Antibacterial Evaluation of Bioactive Compounds from Garcinia kola (Heckel) Seeds

Identification and Antibacterial Evaluation of Bioactive Compounds from Garcinia kola (Heckel) Seeds

Hydnora africana Derived Phytochemicals against Acetaldehyde Dehydrogenase of Aeromonas hydrophila Causing Septicemia

<i>Gossypium hirsutum</i> L. and <i>Gossypium barbadense</i> L.: differences in phytochemical contents, antioxidant and antimicrobial properties.

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