Bioactivity of Samsum ant (Pachycondyla sennaarensis) venom against lipopolysaccharides through antioxidant and upregulation of Akt1 signaling in rats

Abstract

BackgroundThis study aimed at investigating the oxidative stress ameliorating effect, lipids profile restoration, and the anti-inflammatory effect of Samsum Ant Venom (SAV) in induced endotoxemic male rats, injected with bacterial lipopolysaccharides (LPS).ResultsResults revealed that LPS significantly increased the oxidative stress indications in LPS-injected rats. A significant increase of both malondialdehyde (MDA), and advanced oxidative protein products (AOPP), as well as a significant suppression of glutathione were all detected. Treatment with 100 μg/kg dose of SAV significantly restored the oxidative stress normal indications and increased the total glutathione levels. Treatment of the LPS-rats with 100 μg/kg dose of SAV showed a clear anti-inflammatory function; as the histological architecture of the hepatic tissue was partially recovered, along with a valuable decrease in the leukocytes infiltrated the hepatic tissues. Treatment of some rat groups with 600 μg/kg dose of SAV after LPS injection induced a severe endotoxemia that resulted in very high mortality rates. SAV versus the effects of LPS on AKT1, Fas, TNF-α and IFN-γ mRNA expression. SAV was found to significantly lower Fas gene expression comparing to the LPS group and restore the level of IFN-γ mRNA expression to that of the control group.ConclusionIn conclusion, SAV, at the dose of 100 μg/kg body weight, maintained and restored the oxidative stability, the anti-inflammatory, and the hypolipidemic bioactivity in rats after induced disruption of these parameters by LPS injection. This improvement by SAV was mediated by upregulation of AKT1.

Potential effects of samsum ant, Brachyponera sennaarensis, venom on TNF-α/NF-κB mediated inflammation in CCL4-toxicity in vivo

“…We found that the level of MDA was elevated in diseased rats while, SAV restored the oxidative markers in these rats. Previous studies have shown that SAV plays an active role in scavenging free radicals [26]. Indeed, a significant enhancement of glutathione and catalase was observed in the SAV supplemented rats in this study.…”

Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response

“…Our previous work based on the proteins derived from camel milk have demonstrated their enhanced wound healing capacity in diabetic as well as older animals [ 13 – 15 ]. Additionally, our recent study [ 16 ] revealed the ability of this protein to direct immune processes and its ability to trigger the proliferation of peripheral blood mononuclear cells (PBMCs) [ 17 ]. In the present piece of work, we hypothesize that wound healing in diabetic animals could be improved by supplementing camel milk derived protein fractions.…”

Comparisons of Protein and Peptide Complexity in Poneroid and Formicoid Ant Venoms

“…The limited work done thus far reveals that ant venoms show enormous chemical diversity comprising alkaloids, hydrocarbons, proteins and peptides 3,4 . Most studies on ant venoms have focused on the alkaloidal components, as these are a major cause of allergic reactions due to their histamine-releasing properties 13,14 . However, more recent studies have shown the involvement of the proteome in eliciting these allergic 15,16 and antiinflammatory 17,18 properties.…”