Bioadhesion of gelatin films crosslinked with glutaraldehyde

Abstract

The present study was carried out in an attempt to make a gelatin film strongly bioadhesive by introducing free dangling aldehyde groups. When gelatin films were treated with 0.5M of glutaraldehyde (GA) solution at 60 degrees C, free aldehyde groups (up to 150 micromol/g) were introduced in the film. The bonding strength of GA-crosslinked gelatin films (GA gelatin films) with biological tissue was assessed using porcine skins. It was found that bonding strength increased with increasing aldehyde content in the film. The GA gelatin films had bonding strength as high as 250 gf/cm2 whereas the native gelatin film (before GA treatment) showed bonding strength of 40 gf/cm2. When the aldehyde groups introduced in the gelatin films were quenched with glycine or reduced by NaBH4, the films no longer demonstrated such high bonding strength. These facts suggest that a Schiff base was formed between the free dangling aldehyde in the GA gelatin films and the amino groups of the natural tissue, which strongly contributed to a marked bioadhesion.

DNA-Chitosan Bilayer Membrane as a Bi-functional Biomedical Adhesive

“…The tensile strength of more than 2.0 mg cm −2 chitosan membranes showed higher than 3.0 N indicating to have a potential to use for a surgical tape. 10 Based on the results, we used 2.0 mg cm −2 chitosan membrane for further experiments.…”

“…The DNA-chitosan bilayer membranes were suggested to have enough tensile strength as a surgical tape. 10 Next, we observed the surface of the DNA-chitosan membranes using a SEM (Figure 4). The UV-irradiated DNA membrane showed fiber-like surface structure ( Figure 4 Va and Vb).…”

“…Various biomedical adhesives, such as cyanoacrylate polymer, fibrin glue, chemically crosslinkable gelatin, and photo-crosslinkable chitosan, have been developed. 10,11 Cyanoacrylate polymer has connected the organs by the strong adhesion on wet surface of tissue, however, it has serious drawbacks to be obstructive the progress of wound healing by hard solid and also to produce formaldehyde by degradation of cyanoacrylate. 12,13 Although fibrin glue using blood coagulation systems containing fibrinogen, thrombin, factor XIII, and protease inhibitor, has been attracted as a friendly suture material for body, it has been reported on risky infection, because it originated from the blood of human and cattle.…”

Glutaraldehyde cross-linking of amniotic membranes affects their nanofibrous structures and limbal epithelial cell culture characteristics

“…14 Furthermore, Matsuda et al have reported that the aldehyde groups introduced in the cross-linked biopolymers can be quenched with glycine to reduce the cytotoxicity of chemical compounds. 15 Our previous study has also shown that human retinal pigment epithelial cell viability is significantly increased for GTA cross-linked gelatin samples with glycine treatment compared with their counterparts without glycine treatment. 16 Therefore, in this study, the AM materials following GTA cross-linking are placed in glycine aqueous solution for the capping of residual aldehyde groups.…”

“…Reverse transcription of the extracted RNA (1 µg) was performed using ImProm-II™ (Promega) and Oligo(dT) 15 primers (Promega). The primers used to amplify the human interleukin 6 (IL-6) complementary DNA (cDNA) were 5′-CCACTCACCTCT-TCAGAACGAA-3′ (sense) and 5′-GGCAAGTCTC-CTCATTGAATCC-3′ (antisense).…”