Bioadhesive vaginal drug delivery of nystatin using a derivatized polymer: Development and characterization

Bassi, Pallavi; Kaur, Gurpreet

doi:10.1016/j.ejpb.2015.07.018

Cited by 44 publications

(20 citation statements)

References 47 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although Mo -CBP 2 is toxic to Candida spp., this protein did not cause hemolysis in human and rabbit erythrocytes, even in the highest concentration tested (1000 μg/mL). Despite the nystatin effectiveness as antifungal agent and prescription in superficial candidiasis treatment, its clinical use is limited due to its toxicity to human erythrocytes (Bassi and Kaur, 2015). Melittin, other potent antimicrobial peptide against Candida spp., causes hemolysis in human blood cells even in low concentrations, which limits its use in antifungal therapy (Lee and Lee, 2015; Lee et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

A Chitin-binding Protein Purified from Moringa oleifera Seeds Presents Anticandidal Activity by Increasing Cell Membrane Permeability and Reactive Oxygen Species Production

et al. 2017

View full text Add to dashboard Cite

Candida species are opportunistic pathogens that infect immunocompromised and/or immunosuppressed patients, particularly in hospital facilities, that besides representing a significant threat to health increase the risk of mortality. Apart from echinocandins and triazoles, which are well tolerated, most of the antifungal drugs used for candidiasis treatment can cause side effects and lead to the development of resistant strains. A promising alternative to the conventional treatments is the use of plant proteins. M. oleifera Lam. is a plant with valuable medicinal properties, including antimicrobial activity. This work aimed to purify a chitin-binding protein from M. oleifera seeds and to evaluate its antifungal properties against Candida species. The purified protein, named Mo-CBP2, represented about 0.2% of the total seed protein and appeared as a single band on native PAGE. By mass spectrometry, Mo-CBP2 presented 13,309 Da. However, by SDS-PAGE, Mo-CBP2 migrated as a single band with an apparent molecular mass of 23,400 Da. Tricine-SDS-PAGE of Mo-CBP2 under reduced conditions revealed two protein bands with apparent molecular masses of 7,900 and 4,600 Da. Altogether, these results suggest that Mo-CBP2 exists in different oligomeric forms. Moreover, Mo-CBP2 is a basic glycoprotein (pI 10.9) with 4.1% (m/m) sugar and it did not display hemagglutinating and hemolytic activities upon rabbit and human erythrocytes. A comparative analysis of the sequence of triptic peptides from Mo-CBP2 in solution, after LC-ESI-MS/MS, revealed similarity with other M. oleifera proteins, as the 2S albumin Mo-CBP3 and flocculating proteins, and 2S albumins from different species. Mo-CBP2 possesses in vitro antifungal activity against Candida albicans, C. parapsilosis, C. krusei, and C. tropicalis, with MIC50 and MIC90 values ranging between 9.45–37.90 and 155.84–260.29 μM, respectively. In addition, Mo-CBP2 (18.90 μM) increased the cell membrane permeabilization and reactive oxygen species production in C. albicans and promoted degradation of circular plasmid DNA (pUC18) from Escherichia coli. The data presented in this study highlight the potential use of Mo-CBP2 as an anticandidal agent, based on its ability to inhibit Candida spp. growth with apparently low toxicity on mammalian cells.

show abstract

Section: Discussionmentioning

confidence: 99%

A Chitin-binding Protein Purified from Moringa oleifera Seeds Presents Anticandidal Activity by Increasing Cell Membrane Permeability and Reactive Oxygen Species Production

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The dosage form needs to reside at the site of infection for a prolonged period. These limitations can be overcome by a drug delivery system because of portability, convenient application, long retention time, easy storage and handling and improved stability [18].…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica MCM-41 as a Carriers Material for Nystatine Drug in Delivery System

Albayati¹,

Alkafajy²

2019

alkej

View full text Add to dashboard Cite

In the present study, MCM-41 was synthesis as a carrier for poorly drugs soluble in water, by the sol-gel technique. Textural and chemical characterizations of MCM-41 were carried out by X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscope (SEM), and thermal gravimetric analysis (TGA). The experimental results were analyzed mesoporous carriers MCM-41. With maximum drug loading efficiency in MCM-41 determined to be 90.74%. The NYS released was prudently studied in simulated body fluid (SBF) pH 7.4 and the results proved that the release of NYS from MCM-41 was (87.79%) after 18 hr. The data of NYS released was found to be submitted a Weibull model with a correlation coefficient of (0.995). The Historical data experimental design facilitated the formulation and optimization of sustained discover the optimal formulation to loading drug, combine process variables, mixture components and categorical factors in one design.

show abstract

“…In the past two decades, there has been growing research interest in developing new drug formulations that are more accepted by patients, by reducing the frequency of doses and improving the organoleptic properties, in particular regarding the use of nys to treat oral candidiasis [18,[31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Pre-formulation Study for Preparation of Mucoadhesive Buccal Tablets Containing Nystatin and Cashew Gum by Direct Compression

Magalhães¹,

Carmo²,

Mansur³

2019

JPSA

View full text Add to dashboard Cite

Cashew gum is a branched chain heteropolysaccharide extracted from the cashew tree (Anacardium occidentale L.). Purified cashew gum (PCG) is free of plant contaminants and is highly soluble. Several studies have indicated this polymer can be relevant in the pharmaceutical industry for production of tablets. Recently, our research group reported that PCG can be used as a diluent for tablets produced by direct compression. Nystatin (Nys) is the drug of first choice for treatment of oral candidiasis, in the form of a suspension. The treatment consists of up to six daily doses of a suspension of nys at 500,000 IU, causing low therapeutic adhesion by patients. The objective of this study was to investigate the behavior of PCG together with nys and other excipients (flavoring agents and lubricating agent) for future manufacture of mucoadhesive buccal tablets by direct compression. For that purpose, we performed pre-formulation tests (FTIR, TGA, XRD, solubility, pH, granulometry, swelling degree and powder flow) with physical mixtures of the drug and excipients. The results were excellent, demonstrating that PCG is a polymer with potential for this type of application.

show abstract

Bioadhesive vaginal drug delivery of nystatin using a derivatized polymer: Development and characterization

Cited by 44 publications

References 47 publications

A Chitin-binding Protein Purified from Moringa oleifera Seeds Presents Anticandidal Activity by Increasing Cell Membrane Permeability and Reactive Oxygen Species Production

A Chitin-binding Protein Purified from Moringa oleifera Seeds Presents Anticandidal Activity by Increasing Cell Membrane Permeability and Reactive Oxygen Species Production

Mesoporous Silica MCM-41 as a Carriers Material for Nystatine Drug in Delivery System

Pre-formulation Study for Preparation of Mucoadhesive Buccal Tablets Containing Nystatin and Cashew Gum by Direct Compression

Contact Info

Product

Resources

About