Bioadsorption and microbe-mediated reduction of Sb(V) by a marine bacterium in the presence of sulfite/thiosulfate and the mechanism study

Abstract

Microbe-Sb interactions remain poorly understood and the molecular mechanism of microbe-mediated Sb(V) reduction remains unclear. In the present study, we investigated the process and mechanism of Sb(V) bioreduction by Shewanella sp. CNZ-1, which was isolated from the sediment of the Bohai Strait, in the absence or presence of sulfate/sulfite/thiosulfate. Results demonstrate that Sb(V) could be reduced to Sb(III) (including Sb 2 O 3 and Sb(III)(aq) etc.) by CNZ-1 cells. Kinetic studies are carried out using the Langmuir-Freundlich dual model and Monod model and the results reveal that Sb removal by strain CNZ-1 is a fast bioadsorption (K LF , 1/n and a are 8.03, 0.0032 and 0.95, respectively; R 2 = 0.98) and slow bioreduction (R 2 = 0.95) process. Moreover, the supplementation of SO 3 2− and S 2 O 3 2− into the reaction system can lead to the formation of Sb 2 S 3 along with the reduction of SO 3 2− and S 2 O 3 2− , while SO 4 2− can't. The precipitates of Sb 2 S 3 and Sb 2 O 3 are further characterized and confirmed by SEM-EDX, XPS and XRD. In addition, global transcriptome assays reveal that genes encoding dehydrogenase, cytochrome, reductase, stress resistance protein, membrane proteins and transporters play key roles during the transformation of SO 3 2− /S 2 O 3 2− by CNZ-1 cells and subsequent RT-qPCR assays show that the genes S.CNZ.1GM004058 and S.CNZ.1GM001069 should account for the reduction of SO 3 2− and S 2 O 3 2− by the CNZ-1 strain, respectively. 1. Introduction Recently, antimony (Sb) has received much attention because of its increasing environmental concerns [1-4]. Sb is the ninth-most mined metal worldwide, and it is used in a diverse array of industries, including the manufacturing of semiconductors, lead acid automobile batteries, printing presses, ammunition, glassware, ceramics and paint pigments [1,5]. The wide use of Sb and Sb-contained compounds has

A novel green approach for fabricating visible, light sensitive nano-broccoli-like antimony trisulfide by marine Sb(v)-reducing bacteria: Revealing potential self-purification in coastal zones

“…The concentrations of Sb species and the dissolved sulfide were analyzed as described in our previous study [22]. The products of photo-catalytic reaction were analyzed using mass spectrum fitted with Sapphire C18 column (4.6 mm × 200 mm).…”

“…Compared to the detailed data on microbial reduction of S species, however, the knowledge of microbial Sb(V) reduction is much limited [27]. To the best of our knowledge, there are only three reported pure culture bacteria that capable of reducing Sb(V) to Sb(III) [20][21][22]. Recently, more and more studies have focused on the reduction of high valence metal ions with marine microorganisms, due to their strong adaptability to bad environment as they get exposure to such unfavorable conditions naturally [28].…”

“…Then Sb 2 S 3 is one of the probably reduction products if some of the above-mentioned microbes can also reduce Sb(V) to Sb (III). If not, in consideration of only three pure cultured bacteria that are capable of reducing Sb(V) to Sb(III) were isolated so far [20][21][22], the SRB and IRB are of particular concern in fabricating biosynthetic antimony trisulfide (bio-Sb 2 S 3 ) in the presence of Sb(III). On the basis of these hypotheses, we conducted the present study with the aim of (i) developing a novel biological approach to fabricate Sb 2 S 3 ; (ii) characterizing the as-prepared bio-Sb 2 S 3 ; (iii) discussing the key population with the ability of fabricating Sb 2 S 3 .…”

Reduction of Sb(V) by coupled biotic-abiotic processes under sulfidogenic conditions

“…Sb(V)-reducing bacteria and archaea have been identified in, or isolated from, soils and sediments from a wide range of aquatic and terrestrial environments (Abin and Hollibaugh 2014;Kulp et al, 2014;Nguyen and Lee 2014;Lai et al 2016Lai et al , 2018Nguyen et al, 2018;Wang et al, 2018;Zhang and Hu 2019), suggesting that direct (enzymatic) microbial reduction of Sb(V) may be a significant component of Sb biogeochemical cycling, particularly in environments with low S. Our results suggest that in natural and engineered environments with sufficient S to support active S redox cycling, Sb(V) reduction may be driven primarily by a coupled biotic-abiotic process wherein biogenic sulfide species are the primary reductants, which is consistent with similar observations from field and laboratory studies (Wang et al, 2013;Couture et al, 2015;Zhang et al, 2016;Liang et al, 2018). Moreover, our results also suggest that the presence of Fe(III) oxides in sulfidogenic environments can impact the distribution of Sb(III) species (e.g., sorbed Sb(III) versus Sb 2 S 3 precipitation), which has implications for the fate and transport of Sb.…”

“…In the majority of these species, oxidation of Sb(III) to Sb(V) is linked to an enzymatic process for detoxification; however, at least two species have been shown to link Sb(III) oxidation to chemoautotrophic growth (Lialikova 1974;Terry et al, 2015). Several bacteria capable of using Sb(V) as a terminal electron acceptor for anaerobic respiration have been identified in both pure culture or as members of enriched microbial consortia (Nguyen and Lee 2014;Lai et al, 2016;Abin and Hollibaugh 2017;Nguyen et al 2018Nguyen et al , 2019Zhu et al, 2018;Zhang and Hu 2019;Yang et al, 2020), as well as an archaeon that can use Sb(V) as an electron acceptor for anaerobic methane oxidation (Lai et al, 2018). In addition to direct microbial redox transformations of Sb(III) and Sb(V), microbial processes can indirectly impact Sb speciation/mobility by altering the geochemical conditions of their environment, particularly microbes involved in the biogeochemical cycling of Fe and S. For example, reductive dissolution of Fe(III) oxides by dissimilatory Fe(III)-reducing bacteria (DIRB) could lead to release of sorbed Sb(V) and potential reduction to Sb(III) by Fe(II)-bearing secondary minerals such as magnetite or incorporation of Sb(V) into more crystalline secondary Fe(III) oxides (Burton et al, 2019).…”