Bioagent consortia assisted suppression in grey blight disease with enhanced leaf nutrients and biochemical properties of tea (Camellia sinensis)

Bora, Popy; Bora, L. C.; Bhuyan, R.; Hashem, Abeer; Abd_Allah, Elsayed Fathi

doi:10.1016/j.biocontrol.2022.104907

“…In our study, combination of Pf and Th expanded the functional corridor of these two antagonists via ammonia production, phosphate solubilization, and indole acetic acid production, either in absence or in the presence of chlorpyriphos, thereby, facilitated in optimizing the performance of host crop. Some of our recent studies have strongly advocated the application of microbesin a consortium mode proved far more efficacious ( Bora et al 2022 ;Singh et al 2021) , since brinjal plants bioprimed with Trichoderma sp. and challenged with Sclerotinia sclerotiorum were observed having higher amount of shikimic acid coupled with defense-related enzymes viz., polyphenol oxidase, peroxidase, and phenylalanine ammonia lyase ( Bora and Bora 2021) .…”

Section: Discussionmentioning

confidence: 99%

Microbes assisted management of bacterial wilt of brinjal in pesticide contaminated soil

Ahmed

¹

,

Bora

²

2023

Preprint

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Growing brinjal (Solanum melongena L.) in a soil polluted with organophosphorus pesticide (OP) residues coupled with an additional threat of wilt disease caused by Ralstonia solanacearum (Rs), pose a formidable management challenge for residue free crop production . Our study aimed at identifying efficient OP-degrading bacteria (OPDB) and their compatibility with microbial bio-control agents (MBCA) for twin objective of microbial degradation of OP -residues and reduction in incidence of bacterial wilt of brinjal in OP- contaminated soil. As many, ten bacterial isolates showing OP- degrading potential were recovered through colony growth in mineral salt (MS) -medium treated with 25 ppm chlorpyriphos. Efficient isolates displaying growth up to 700ppm chlorpyriphos were further screened for OP-degradation, leading to identification of two most efficient OPDB, Acromobacter marplatensis [MW397524] (Am) and Pseudomonas azotoformans [MW397525] (Pa). These two isolates having in vitro compatibility with each other, showed further compatibility with two most widely used MBCA, viz., Pseudomonas flourescens [KT258013] (Pf) and Trichoderma harzianum [ON364138] (Th), facilitating the development of consortium having extended functional corridor. The response of combination of OPDB + MBCA(Am +Pa + Pf + Th) showed 80% lower percent wilt incidence (PWI), 2.8 times higher fruit yield ofbrinjal, and 15-25% lower OP-residues over un-inoculated control treatment. Our studies, hence, put forward a strong unified delivery mechanism of OPDB and MBCA as a part of green technology for chemical residue -free vegetable production in contaminated soils.

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“…In our study, combination of Pf and Th expanded the functional corridor of these two antagonists via ammonia production, phosphate solubilization, and indole acetic acid production, either in absence or in the presence of chlorpyriphos, thereby, facilitated in optimizing the performance of host crop. Some of our recent studies have strongly advocated the application of microbesin a consortium mode proved far more efficacious (62,63) , since brinjal plants bioprimed with Trichoderma sp. and challenged with Sclerotinia sclerotiorum were observed having higher amount of shikimic acid coupled with defenserelated enzymes viz., polyphenol oxidase, peroxidase, and phenylalanine ammonia lyase (60) .…”

Section: Discussionmentioning

confidence: 99%

Microbial Remediation of Pesticide Accumulation and its Response of Bacterial Wilt in Brinjal

Bora

¹

,

Ahmed

²

2023

Preprint

0

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Growing brinjal (Solanum melongenaL.) in a soil polluted with organophosphorus pesticide (OP) residues coupled with an additional threat of wilt disease caused byRalstonia solanacearum(Rs), pose a formidable management challenge for residue free crop production. Our study aimed at identifying efficient OP-degrading bacteria (OPDB) and their compatibility with microbial bio-control agents (MBCA) for twin objective of microbial degradation of OP -residues and reduction in incidence of bacterial wilt of brinjal in OP-contaminated soil. As many, ten bacterial isolates showing OP-degrading potential were recovered through colony growth in mineral salt (MS) - medium treated with 25 ppm chlorpyriphos. Efficient isolates displaying growth up to 700ppm chlorpyriphos were further screened for OP-degradation, leading to identification of two most efficient OPDB,Acromobacter marplatensis[MW397524] (Am) andPseudomonas azotoformans[MW397525] (Pa). These two isolates havingin vitrocompatibility with each other, showed further compatibility with two most widely used MBCA, viz.,Pseudomonas flourescens[KT258013] (Pf) andTrichoderma harzianum[ON364138] (Th), facilitating the development of consortium having extended functional corridor. The response of combination of OPDB + MBCA(Am+Pa+Pf+Th) showed 80% lower percent wilt incidence (PWI), 2.8 times higher fruit yield of brinjal, and 15-25% lower OP-residues over un-inoculated control treatment. Our studies, hence, put forward a strong unified delivery mechanism of OPDB and MBCA as a part of green technology for chemical residue -free vegetable production in contaminated soils.IMPORTANCEMicrobes isolated from crop rhizospheres are known to exhibit multiple functions (plant growth promotion, antagonism against soil borne pathogens e.g.,Rs,and acaricidal properties) depending upon type of stimuli by regulating the plant defense. Considering the limited past efforts on twin objective of cleaning up the pesticide residues accumulated in the soil (microbial removal of pesticide residues) and providing an additional safeguard against soil borne pathogen causing bacterial wilt in brinjal to collectively develop a microbial consortia mediated crop production system, devoid of any chemical residues. Such an effort adds strength to organic production system on pesticide contaminated soils. In the present study, we succeeded in identifying two most effective chloropyrifos residues degrading OPDB having compatibility two MBCA for developing consortia formulation, which aided in developing pesticide residue free soil, neutralising bacterial wilt pathogen (Rs), and creating a better nutrient supply for a well deserved production system. Such efforts would pave the way for developing more robust microbial formulations (with emphasis on entomopathogenic application) having dynamic microbial functions to replace any futuristic use of agrochemcials.

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“…"Alternative probable mechanisms of the antagonistic function of bioagent can be attributed to the synthesis of extracellular hydrolases such as chitinases and β-1,3-glucanases capable of destroying the structural polysaccharides in the cell wall (chitin and glucans) of fungus and lysing the hyphae of fungi" [76,77,78]. "For several bacteria, a correlation between antagonistic activity to various pathogenic fungi and the synthesis of cellulases, mannanases, xylanases, proteases, and lipases has also been established.…”

Section: Synthesis Of Hydrolytic Enzymesmentioning

confidence: 99%

Biocontrol Agents against Post Harvest Decay in Fruits and Vegetables: A Review

Taye

¹

,

Saikia

²

,

Panging³

2023

IJPSS

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Post harvest losses are the major threats in the supply chain between harvest and consumption which contribute 44% of the total loss of fruits and vegetables. Among the various causes, post harvest diseases are major decay of fruits and vegetables accounting for 20-25% losses. The causative fungus belonging to both biotrophic and necrotrophic nature belongs to the genera of Aspergillus, Penicillium, Botrytis, etc. Management of post harvest diseases by conventional chemicals is not preferred due to residual effects and toxicity. Considering the global demand and consumer awareness about the health effects of pesticides, biocontrol agents are getting attention in recent times for post harvest disease management. BCAs like Trichoderma, Bacillus and Pseudomonas have been explored for successful management of post harvest diseases of citrus, strawberry, tomato, etc. The possible mode actions of the BCAs are competition for nutrients and space, production of antimicrobial compounds, hydrolytic enzymes, and induced resistance. The added advantage of BCAs is that they can be integrated with other physical, natural compounds and additives for coatings due to their synergistic and mutualistic effect. The product development for biocontrol origin must be encouraged to utilize the benefits they provide. Several constraints in process of product development may arise which can be overcome by more research, education, training at the farm level, and multi-omics studies to unravel the potentials of the BCAs.

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Bioagent consortia assisted suppression in grey blight disease with enhanced leaf nutrients and biochemical properties of tea (Camellia sinensis)

Cited by 12 publications

References 47 publications

Microbes assisted management of bacterial wilt of brinjal in pesticide contaminated soil

Microbes assisted management of bacterial wilt of brinjal in pesticide contaminated soil

Microbial Remediation of Pesticide Accumulation and its Response of Bacterial Wilt in Brinjal

Biocontrol Agents against Post Harvest Decay in Fruits and Vegetables: A Review

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