Bioanalysis of trifluoperazine in human plasma by LC–MS/MS: Application to disposition kinetics

Abstract

A sensitive, selective and rapid bioanalytical method using liquid chromatography–tandem mass spectrometry has been developed for the quantification of trifluoperazine in human plasma. Trifluoperazine‐D8 was used as the internal standard and the extraction from human plasma was performed by liquid–liquid extraction technique using tertiary butyl methyl ether as the solvent. Chromatographic separation was carried out on Zodiac C18 column (50 × 4.6 mm, 3 μm) employing a mixture of acetonitrile, methanol and 5 mm ammonium bicarbonate buffer in water (85:10:5, v/v/v) at a flow rate of 0.55 ml/min. The linearity was established within the concentration range of 5–1,250 pg/ml with r2 > 0.99. The results of all of the validation parameters as per the US Food and Drug Administration guidelines were within the acceptance limits. The pharmacokinetics of trifluoperazine after oral administration of a syrup of 1 mg dose under fasting conditions was determined by successful application of the present method.

Electrochemical sensor for simultaneous determination of trifluoperazine and dopamine in human serum based on graphene oxide–carbon nanotubes/iron–nickel nanoparticles

“…7,8 Some methods are utilized for TFLP detection such as reverse phase HPLC method, liquid chromatographytandem mass spectrometry, spectrophotometry, ow injection uorimetry. [9][10][11][12] Low cost, simple operation, sensitivity, and fast response are the advantages of electrochemical methods over other cited methods. 13,14 Carbon nanotubes (CNTs) are made of concentric cylinders of graphite layers.…”