Bioanalytical applications of ?fast chromatography? to high-throughput liquid chromatography/tandem mass spectrometric quantitation

Development of generic liquid chromatography-mass spectrometry methods using experimental design

“…Drug discovery today requires high throughput analytical techniques, such as LC-MS and LC-MS-MS [1,2] for in vitro and in vivo assays for the many chemically diverse compounds being studied. A particular area of interest is the analysis of compounds with only one ionizable group which is acidic.…”

Identifying and Overcoming Matrix Effects in Drug Discovery and Development

“…Ye et al used a mixture of methanol and ACN as the organic mobile phase, to minimize phospholipid-related matrix effects and maintain high sample throughput (Ye et al, 2011). Recently, an ultra-fast gradient approach also referred to as a "ballistic" gradient, allowed for analysis cycle times of 2 minutes or less (Romanyshyn and Tiller, 2001;Romanyshyn et al, 2000). Tiller and Romanyshyn studied matrix effects encountered using ultra-fast gradients and fast isocratic methods.…”

The effect of eluent pH and compound acid–base character on the design of generic-gradient reversed-phase high-performance liquid chromatography (RP-HPLC) methods for use in drug discovery

“…The measurement of physicochemical, metabolic or biopharmaceutical properties of drug candidates typically involves the use of gradient reversed-phase high-performance liquid chromatography (RP-HPLC) [1][2][3][4]. In an endeavour to eliminate method development time and maximise throughput, many pharmaceutical laboratories are employing generic HPLC E-mail address: brian.law@astrazeneca.com (B.…”