Bioanalytical liquid chromatography–tandem mass spectrometry method development and validation for quantification of lurasidone in rat plasma using ion pairing agent

Abstract

A bioanalytical method was developed and validated for determining lurasidone (LUR) in rat plasma. The analyte and internal standard were extracted from rat plasma using a liquid–liquid extraction method. The mobile phase consisted of methanol, acetonitrile and water, with an ion pairing agent, 0.1% heptafluorobutyric acid, added to minimise the matrix effect. The detection was achieved using a tandem mass spectrometer (API 2000) in positive ion multiple reaction monitoring mode. All parameters were validated, including selectivity, specificity, carry‐over effect, linearity, precision, accuracy, matrix effect, sensitivity and stability. The linearity range was from 5.0 to 1200.0 ng/mL with a correlation coefficient of >0.99. The accuracy ranged from 100.00% to 110.22% across the quality control range. The mean absolute recovery from matrix samples for LUR and the internal standard was found to be 68.46% and 67.25%, respectively, and the relative recovery was found to be 73.89% and 77.44%, respectively. This method can determine LUR concentrations in rat plasma samples up to 12 h after oral administration, aiding in LUR pharmacokinetic (PK) investigations in rats. The method's reproducibility on a conventional LC–MS/MS system and a shorter run time of 3.0 min make it an appealing bioanalytical method for quantifying LUR in PK studies.

Validation of a high‐performance liquid chromatography method for determining lysophosphatidylcholine content in bovine pulmonary surfactant medication

“…Finally, owing to the importance of method validation (Beg et al, 2016;Brown, 2024;Chen et al, 2024;Chintala, 2024;Dar et al, 2022;Davis et al, 2024;Heudi & Winter, 2024;Hussien, 2014;Rajadhyaksha & Londhe, 2024;Sharma et al, 2012;Xue et al, 2012), the method underwent comprehensive validation for eight parameters. Importantly, the execution of this analytical method does not depend on any state-of-the-art equipment.…”