Bioanalytical Qualification of Clinical Biomarker Assays in Plasma Using A Novel Multi-Analyte Simple Plex ^™ Platform

Abstract

The qualified assays will be useful in assessing mechanism of action cancer immunotherapies.

A novel multiple biomarker panel for the early detection of high-grade serous ovarian carcinoma

“…Our study is one such effort to identify a panel of multiple serum biomarkers which is not just sensitive and specific, but also time-efficient and cost-effective. Simple Plex is a new multi-analyte immunoassay platform, which has been proven to have significant advantages over traditional approaches in terms of low volume requirements (2.5 to 25 μL), sensitivity, and reproducibility [34, 35]. Simple Plex eliminates inconsistency in sample handling and produces results in a short amount of time, thus providing results with minimal error and in timely fashion.…”

CD8+ T cell-intrinsic IL-6 signaling promotes resistance to anti-PD-L1 immunotherapy

“…Plasma IL-6 was evaluated by previously qualified immunoassays on the multi-analyte platform Simple Plex Ella. 70 The samples were diluted twofold in sample diluent and loaded onto the cartridge for data acquisition.…”

Biomarqueurs prédictifs de réponse aux inhibiteurs de points de contrôle immuns

“…Une étude [69] dernièrement présenté à l'ASCO, constatant que les lymphocytes activés relarguaient certaines cytokines (IFN-gamma, IL2-4-5-10, TNF-alpha et béta) favorisant l'afflux de cellules immunitaires sur le site tumoral et augmentant la reconnaissance des cellules tumorales par les cellules cytotoxiques, a décrit un test permettant de mesurer les taux de cytokines avec une bonne sensibilité et une bonne spécificité. Ce test pourrait donc servir d'outil préférentiel pour la mesure de cytokines prédictives de réponse.…”