Bioanalytical strategies for in-vitro and in-vivo evaluation of the toxicity induced by metallic nanoparticles

Abstract

The increasing use of metallic nanoparticles (MNPs) in a wide variety of applications have consequently lead to an urgent need to evaluate the impact of these new materials on human health and the environment. To date, the potential toxicity of MNPs as well as their interaction mechanisms with cells and living organisms have not been fully addressed. In this article, we discuss the different bioanalytical strategies that have been used so far with this purpose. We consider different methods aim to evaluate cellular uptake and localization in cells and tissues, and in vitro methods for the study of the toxicity induced by MNPs considering different toxicity markers and high throughput approaches for the identification of specific targets involved in the cell-MNPs interaction. Special strategies related to the use of animal models to assess in-vivo toxicity of MNPs are also discussed.

Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles

“…186 In another study, in vitro and in vivo studies for determination of toxicity of metallic nanoparticles were performed and it was observed that the nature of nanoparticle like size, shape and stability can affect toxicity level. 187 But results for in vivo and in vitro study was not in good correlation. It may have been due to tested cell line.…”

Ecological risk assessment of silicon dioxide nanoparticles in a freshwater fish Labeo rohita: Hematology, ionoregulation and gill Na+/K+ ATPase activity

“…To date, the impact of metallic nanoparticles (MNPs) on human health and the environment and also their interaction with living organisms are very limited (Petersen et al, 2008;Luque-Garcia et al, 2013). Silica-based materials or silica nanostructure are widely used in many biotechnological and biomedical applications such as biosensor design, drug delivery, cell labeling, cell separation, and ultrasound medical imaging (Parkinson and Gordon, 1999;Drum and Gordon, 2003;Gordon et al, 2009;Van Hoecke et al, 2011;Park et al, 2013).…”

In vitro and in vivo experiments with iron oxide nanoparticles functionalized with DEXTRAN or polyethylene glycol for medical applications: Magnetic targeting

“…In total, each experiment was performed with 10,000 cells in average, and the viability was assessed as a function of the concentration of the SPIONs in the medium (μg of SPIONs per mL of medium—μg/mL). Fibroblast cell lines have been used to determine toxic effects induced by nanoparticles as they are the major cellular constituent of fibrous connective tissue, and Vero and MDCK cells have frequently been used with cytotoxicity assays with the advantages of fast grown and easy access …”

“…Fibroblast cell lines have been used to determine toxic effects induced by nanoparticles as they are the major cellular constituent of fibrous connective tissue, and Vero and MDCK cells have frequently been used with cytotoxicity assays with the advantages of fast grown and easy access. [30][31][32] In vivo experiments In vivo assays were conducted using a syngeneic transplant animal model consisting in BALB/c mice aged 11 weeks. In vivo assays were conducted using a syngeneic tumor animal model.…”