Bioassay and molecular detection of insecticides resistance of Aedes aegypti, vector of dengue in Central Java Province, Indonesia

Abstract

Abstract. Sayono, Nurullita U, Handoyo W, Tyasningrum WS, Chakim I, Budiharjo A. 2023. Bioassay and molecular detectionsof insecticides resistance of Aedes aegypti, vector of dengue in Central Java Province, Indonesia. Biodiversitas 24: 300-307. The emergence of insecticide-resistant strains among Aedes aegyptipopulations hampered Dengue control programs in the endemic areas. Moreover, to understand the current situation and distribution of insecticide resistance status of Ae. aegypti to cypermethrin, malathion, and temephos compounds, we conducted morphological and molecular detection in the Dengue endemic areas in Central Java Province, Indonesia. Mosquito larvae were obtained from thirteen villages of five Dengue endemic areas representing different altitudes. Larval and adult stage of Ae. aegypti colony from each village was subjected to a bioassay test based on the WHO procedures. Subsequently, they were sampled and subjected to molecular analysis to identify the 1016G kdr allele using the allele-specific polymerase chain reaction (AS-PCR). Mortality of Ae. aegypti after exposure to cypermethrin, malathion, and temephos ranged from 16-86%, 75-100%, and 6-51%, respectively. These findings showed that Ae. aegypti populations were resistant to cypermethrin and temephos, although malathion-susceptible strains were found among 23.08% of the different altitudinal localities. The result of the AS-PCR indicated that the homozygous (G/G) and heterozygous (V/G) alleles of codon 1016 of the AaNav gene were found throughout the study site altitudes. The development of multiple resistance strains was found among Ae. aegypti populations in Central Java Province, Indonesia. The use ofcypermethrin and temephos compounds must be delayed for at least five years, while malathion can still be used selectively to control the Ae. aegypti population in several areas, namely Karangjati, Gebugan (Semarang District), and Rowosari in Semarang City.

First concrete documentation for presence ofAedes (Stegomyia) albopictusin Bolivia: Dispelling previous anecdotes

“…Aedes albopictus displays phenotypic plasticity, enabling the development of strains adapted to a broad range of environmental conditions and optimal temperatures for survival and development (44; 45) . The mosquito has been collected along altitudinal transects, reaching up to 2100m above sea level in Nepal ( 46 ), and is commonly found above 1200 m in tropical environments (47; 48) . With a demonstrated capacity to outcompete Ae.…”

Trends and Research Hotspots of Knockdown Resistance in the Aedes Genus in Indonesia: A Bibliometric Surveillance from the Scopus Database

“…Focus of research Cluster 1 (24 items) -Red Anopheles [27], c1534 [13], chemical [13], culex [17], culex quimquefasciatus [24], culicidae [105], cyfluthrin [10], cyhalothrin [29], deet [12], diptera [115], insecticide susceptibility status [11], kdr gene [11], knock [64], knockdown [123], malaria [25], malaria vector [20], mosquito coil [19], mosquito species [23], piperonyl butoxide [16], pirimiphos methyl [10], repellency [30], repellent [49], resistance ratio [24], transfluthrin [24].…”

“…Identification of kdr at allele f1534c in temephos-exposed Aedes aegypti and Aedes albopictus mosquitoes Cluster 3 (12 items) -Blue Aegypty mosquito [20], bali [21], dengue fever [26], denpasar [21], egg [19], human [15], indonesia [307], jakarta [18], resistance development [28], resistance phenotype [18], surveillance [22], v1016g mutation [14] Mutation analysis of the v1016g allele in dengue fever-causing mosquitoes in Denpasar, Bali and Jakarta Cluster 4 (12 items) -Yellow Aedes aegypti mosquito [31], central java [10], channel [130], cypermetrin [14], dengue vector aedes [12], haplotype [43], ile1016 [10], 1982w [13], s989p [46], vgsc [82], vgsc gene [21], voltage gated sodium channel [15] Identification of kdr in voltage gated sodium channel (VGSC) of Aedes aegypti mosquito Cluster 5 (11 items)purple Genotyping [34], insecticide use [13], resistance allele [12], s996 [10], southeast asia [15], target site resistance [10], v1023g [15], voltage sensitive sodium channel [17], vssc [24], vssc muattion [11], yogyakarta [2] Analysis of voltage sensitive sodium channel (VSSC) in mosquitoes in Yogyakarta Cluster 6 (9 items) -sky-blue Aedes aegypti [10], aegypti strain [12], chikv [11], dengue virus [23], denv [19], primary vector [18], virus …”

Effectiveness of Foeniculum vulgare leaf essential oil nanoemulsion as a natural insecticide against Aedes aegypti larvae and mosquitoes

“…Continuous use of chemical insecticides can cause resistance to both mosquitoes and mosquito larvae (Valle et al 2019;Palomino et al 2022). As Sayono et al (2023) reported, the populations of Ae. aegypti resistant to cypermethrin 0.05% and temephos 0.02% are spread in several dengue endemic areas in Central Java, Indonesia.…”