Bioassay and Molecular Study for Detection of Insecticide Resistance Dengue Causing Mosquito Vectors

Soni, Monika; Bhattacharya, Chandrakanta; Sharma, Jitendra; Dutta, Prafulla

doi:10.4103/ijmm.ijmm_18_120

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…These findings are found to be in concurrence with previous studies where resistance to pyrethrum has been reported but no VGSC gene mutation was detected in spite of a high number of heterozygote genotypes. 25,31,32 In recent studies, it has been documented that the VGSC gene exons 20 and 21 are separated by an intron which is polymorphic based on their base pair length and is classified as intron A and B. It has been reported that polymorphic sites S989P, V1016G, and D1763Y are linked with intron A while the F1534C mutation is linked with the intron B.…”

Section: Discussionmentioning

confidence: 99%

“…FP-5'-AC CGACAAATTGTTTCCC-3';RPG5'-GCGGGCAGGGCGGCGGG GGCGGGGCCAGCAAGGCTAAGAAAAGGTTAACTC-3';RPV 5'-GCGGGCAGCAAGGCTAAGAAAAGGTTAATTA-3'. 25 The cycling conditions were the initial step of denaturation at 94 ºC for 5 mins, followed by 35 cycles of amplification at 94 ºC for 1 min, 58 ºC for 1 min, and 72 ºC for 1 min, and final extension at 72 ºC for 10 mins.…”

Section: V1016g Allele-specific Pcrmentioning

confidence: 99%

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Detection of Insecticide Susceptibility Status and KDR Mutation in Field-Collected Aedes Aegypti from Different Districts of Punjab, India

Grover¹

2022

JCD

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Background: As the state of Punjab has become endemic for dengue, this study was planned to determine the susceptibility status for two different classes of adulticides and the VGSC gene polymorphism in domain II of Aedes aegypti. Methodology: Adult bioassays were performed with pyrethrum, deltamethrin and malathion as per the WHO protocol. AS-PCR and sequencing of VGSC gene were carried out to detect V1016G and Kdr (Knockdown resistance) mutations. Results:Ae. aegypti from the districts of Ludhiana and Patiala were found to be resistant to pyrethrum. The V/ V genotype frequency was found to be higher in all districts. Three known polymorphisms in VGSC gene were not recorded but one synonymous and non-synonymous mutation was found. Group B intron was found in domain II of the VGSC gene. For deltamethrin and malathion, 100% susceptibility was recorded for all four districts. Conclusion: Thus, the present findings indicate 100% susceptibility towards deltamethrin and malathion whereas, moderate resistance towards pyrethrum was recorded. Therefore, more studies should be planned to study polymorphisms in the various domains of the VGSC gene.

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Section: Discussionmentioning

confidence: 99%

Section: V1016g Allele-specific Pcrmentioning

confidence: 99%

Detection of Insecticide Susceptibility Status and KDR Mutation in Field-Collected Aedes Aegypti from Different Districts of Punjab, India

Grover¹

2022

JCD

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“…Vector control is, thus, a crucial part of the global strategy for the management of mosquito-associated diseases, and insecticide application is still the most important component in this effort [ 38 , 39 ]. However, with an intensification of the effort for disease control, mosquitos developed resistance to commonly used insecticides [ [39] , [40] , [41] ] and the fight against the disease spreading has to be re-evaluated. Benelli [ 38 ] has seen the biggest promise in the behavior-based control programs, with special reference to “boosted SIT” (sterile insect technique) and the mosquitocidal botanicals.…”

Section: Introductionmentioning

confidence: 99%

Revisiting the concept of entomotoxicology

Hodecek

2020

Forensic Science International: Synergy

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Until now, the term entomotoxicology has only been used in medico-legal sciences. However, entomotoxicology as a whole has a much wider scope and forensic entomotoxicology is just one of its branches. Based on the literature a wider definition of the term is presented. Today, we can distinguish two major branches of entomotoxicology : 1) Forensic entomotoxicology , which uses the insects as evidence of the presence of xenobiotics in decomposing tissue during an investigation and 2) Environmental entomotoxicology , which uses the insects as bioindicators of environmental pollution in non-criminal circumstances. While forensic entomotoxicology is a relatively new discipline, research in environmental entomotoxicology began as far back as the 1920s. A review of the work in entomotoxicology from the last 6 years is presented, covering several interesting new trends. This article aims to redefine entomotoxicology, which should increase awareness to bring more collaborations and multidisciplinary between related scientific fields.

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Comparative transcriptional analysis between susceptible and resistant populations of Aedes (Stegomyia) aegypti (Linnaeus, 1762) after malathion exposure

de Souza,

Amaro,

Palacio-Cortés

et al. 2024

Mol Genet Genomics

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Bioassay and Molecular Study for Detection of Insecticide Resistance Dengue Causing Mosquito Vectors

Cited by 5 publications

References 7 publications

Detection of Insecticide Susceptibility Status and KDR Mutation in Field-Collected Aedes Aegypti from Different Districts of Punjab, India

Detection of Insecticide Susceptibility Status and KDR Mutation in Field-Collected Aedes Aegypti from Different Districts of Punjab, India

Revisiting the concept of entomotoxicology

Comparative transcriptional analysis between susceptible and resistant populations of Aedes (Stegomyia) aegypti (Linnaeus, 1762) after malathion exposure

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