Bioassay-guided fractionation of Artocarpus heterophyllus L. J33 variety fruit waste extract and identification of its antioxidant constituents by TOF-LCMS

Daud, Mohd Nazrul Hisham; Wibowo, Agustono; Abdullah, Noorlidah; Ahmad, Rohaya

doi:10.1016/j.foodchem.2018.05.120

Cited by 14 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The bioactive compound 1 was isolated from the most active subfraction A2-1 of the active ethyl acetate fraction via a bioassay-guided isolation. As reported in our earlier study [9], subfraction A2-1 possessed a percent inhibition of 92% in the DPPH radical-scavenging assay. The scheme for the bioassay-guided is shown below with the percent of DPPH radical scavenging activity of the fractions and sub-fractions (Figure 1):…”

Section: Isolation Of Bioactive Compoundsupporting

confidence: 80%

“…Based on our previous findings, the rind maceration extract (RDM) of A. heterophyllus J33 (AhJ33) variety fruit possess highest antioxidant ability compared to other extracts [8] and 15 antioxidant constituents were identified via TOF-LCMS [9]. Hence, in this paper we now report the effect of storage on RDM under room, chilled and frozen conditions at a storage period of six months.…”

Section: Submit Manuscriptmentioning

confidence: 90%

See 1 more Smart Citation

Effect of Storage on Antioxidant Activity and Bioactive Compound of Artocarpus heterophyllus J33 Rind Extract

Ltd¹

2019

ACBC

View full text Add to dashboard Cite

In this paper, we report the storage effect of Artocarpus heterophyllus J33 rind extract at room (25°C), chilled (4°C) and frozen (-18°C) conditions on its antioxidant activity, LC profile and quality of bioactive compound. The bioactive compound was isolated via bioassay-guided isolation identified as Protocatechuic Acid (PCA) using spectroscopic methods. After 6 months storage, it was found that the antioxidant activity value of the frozen and chilled extracts remain above 90%, while at room temperature the value was lower. The TIC and LC profiles of the extracts indicated a drop in the content of the bioactive compound. Quantification of the bioactive compound for the extracts (after 6 months storage) revealed that its content was in the order of room < chilled ≤ frozen indicating that, under chilled and frozen conditions, the antioxidant activity of the extracts were prolonged due to the stability of PCA. These findings indicate that A. heterophyllus J33 rind extract could be a potential source of antioxidants for food and nutraceutical products utilizing the waste to wealth concept from agricultural source.

show abstract

Section: Isolation Of Bioactive Compoundsupporting

confidence: 80%

Section: Submit Manuscriptmentioning

confidence: 90%

Effect of Storage on Antioxidant Activity and Bioactive Compound of Artocarpus heterophyllus J33 Rind Extract

Ltd¹

2019

ACBC

View full text Add to dashboard Cite

show abstract

“…Other compounds which exhibited antiplasmodial action include 3,4,3 -tri-O-methylellagic acid, triterpenes: arjunolic acid, arjungenin, and arjunic acid, and phenolic glycoside calophymembranside-B [68]. In addition to the above-mentioned reports, a number of investigations were performed by the natural chemists; where a bioactivity-guided fractionation approach was used for successful isolation of new or previously identified phytoconstituents [69][70][71][72][73][74].…”

Section: Some Recent Experiments Using Bio-activity-guided Fractionation Techniquementioning

confidence: 99%

Modern Approaches in the Discovery and Development of Plant-Based Natural Products and Their Analogues as Potential Therapeutic Agents

et al. 2022

View full text Add to dashboard Cite

Natural products represents an important source of new lead compounds in drug discovery research. Several drugs currently used as therapeutic agents have been developed from natural sources; plant sources are specifically important. In the past few decades, pharmaceutical companies demonstrated insignificant attention towards natural product drug discovery, mainly due to its intrinsic complexity. Recently, technological advancements greatly helped to address the challenges and resulted in the revived scientific interest in drug discovery from natural sources. This review provides a comprehensive overview of various approaches used in the selection, authentication, extraction/isolation, biological screening, and analogue development through the application of modern drug-development principles of plant-based natural products. Main focus is given to the bioactivity-guided fractionation approach along with associated challenges and major advancements. A brief outline of historical development in natural product drug discovery and a snapshot of the prominent natural drugs developed in the last few decades are also presented. The researcher’s opinions indicated that an integrated interdisciplinary approach utilizing technological advances is necessary for the successful development of natural products. These involve the application of efficient selection method, well-designed extraction/isolation procedure, advanced structure elucidation techniques, and bioassays with a high-throughput capacity to establish druggability and patentability of phyto-compounds. A number of modern approaches including molecular modeling, virtual screening, natural product library, and database mining are being used for improving natural product drug discovery research. Renewed scientific interest and recent research trends in natural product drug discovery clearly indicated that natural products will play important role in the future development of new therapeutic drugs and it is also anticipated that efficient application of new approaches will further improve the drug discovery campaign.

show abstract

“…The authors also showed that jackfruit pulp exhibited strong DPPH and OH radical scavenging activities, with a relatively lower reducing power [31]. Similarly, ethyl acetate fraction of jackfruit showed radical scavenging activities between 80% -94% inhibition due to the presence of phenolic compounds [32]. Another study revealed that protocatechuic acid and chlorogenic acid in rind and rachis extracts of jackfruit contribute to the antioxidant activity [33].…”

Section: Antioxidant Activitymentioning

confidence: 93%

Chemical constituents and biological activities of Artocarpus heterophyllus lam (Jackfruit): A review

Bhad¹,

Bobde²,

Sibi³

2021

Int J Clin Microbiol Biochem Technol

View full text Add to dashboard Cite

Artocarpus (Moraceae) is a deciduous tree with appreciable importance as a source of edible fruit and is widely used in folk medicines. The extracts and metabolites of Artocarpus heterophyllus particularly those from leaves, bark, stem and fruit possess several useful bioactive compounds. This review indents to compile various studies on A. heterophyllus and critically evaluates its ethnomedical and ethnopharmacological properties. Several pharmacological studies from A. heterophyllus have conclusively established their mode of action in anti-inflammatory, antimicrobial, antioxidant and anticancer activities. Based on the available data, it is concluded that Artocarpus as a promising source of useful products and opens up new avenues for novel therapeutics.

show abstract

Bioassay-guided fractionation of Artocarpus heterophyllus L. J33 variety fruit waste extract and identification of its antioxidant constituents by TOF-LCMS

Cited by 14 publications

References 33 publications

Effect of Storage on Antioxidant Activity and Bioactive Compound of Artocarpus heterophyllus J33 Rind Extract

Effect of Storage on Antioxidant Activity and Bioactive Compound of Artocarpus heterophyllus J33 Rind Extract

Modern Approaches in the Discovery and Development of Plant-Based Natural Products and Their Analogues as Potential Therapeutic Agents

Chemical constituents and biological activities of Artocarpus heterophyllus lam (Jackfruit): A review

Contact Info

Product

Resources

About