Bioassay-guided fractionation, phospholipase A<sub>2</sub>-inhibitory activity and structure elucidation of compounds from leaves of <i>Schumanniophyton magnificum</i>

Joshua, Parker Elijah; Chizoba, Anosike Joy; Asomadu, Rita Onyekachukwu; Ekpo, Daniel Emmanuel; Uhuo, E. N.; Nwodo, O. F. C.

doi:10.1080/13880209.2020.1839510

Cited by 12 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies suggest that n-hexadecanoic acid has anti-inflammatory effects by inhibiting various inflammatory mediators, including phospholipase A2, prostaglandins E2, interleukin (IL)-6, IL-1, tumor necrosis factor, and nitric oxide synthase. Additionally, it exhibits hypocholesterolemic, nematicidal, and pesticidal effects [ 21 , 22 , 23 , 24 , 25 , 26 ]. The fatty acids identified are in line with the findings from a previous study [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights

Djehiche,

Benzidane,

Djeghim

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

Ammodaucus leucotrichus exhibits promising pharmacological activity, hinting at anti-inflammatory and anti-arthritic effects. This study investigated seed extracts from Ammodaucus leucotrichus using methanol and n-hexane, focusing on anti-inflammatory and anti-arthritic properties. The methanol extract outperformed the n-hexane extract and diclofenac, a reference anti-inflammatory drug, in trypsin inhibition (85% vs. 30% and 64.67% at 125 μg/mL). For trypsin inhibition, the IC50 values were 82.97 μg/mL (methanol), 202.70 μg/mL (n-hexane), and 97.04 μg/mL (diclofenac). Additionally, the n-hexane extract surpassed the methanol extract and diclofenac in BSA (bovine serum albumin) denaturation inhibition (90.4% vs. 22.0% and 51.4% at 62.5 μg/mL). The BSA denaturation IC50 values were 14.30 μg/mL (n-hexane), 5408 μg/mL (methanol), and 42.30 μg/mL (diclofenac). Gas chromatography–mass spectrometry (GC–MS) revealed 59 and 58 secondary metabolites in the methanol and n-hexane extracts, respectively. The higher therapeutic activity of the methanol extract was attributed to hydroxyacetic acid hydrazide, absent in the n-hexane extract. In silico docking studies identified 28 compounds with negative binding energies, indicating potential trypsin inhibition. The 2-hydroxyacetohydrazide displayed superior inhibitory effects compared to diclofenac. Further mechanistic studies are crucial to validate 2-hydroxyacetohydrazide as a potential drug candidate for rheumatoid arthritis treatment.

show abstract

Section: Resultsmentioning

confidence: 99%

Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights

Djehiche,

Benzidane,

Djeghim

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

“…48 9 Hexadecanoic acid Anti-inflammatory and antioxidant. 49 10 Octadecanoic acid Anti-inflammatory 50 and antioxidant. 51,52 11 Oleic acid Antibacterial 53 , anti-inflammatory, antifungal 54 , and anti-cancer.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Profiling of Javanese Ginseng (Talinum paniculatum) Stem Extract Using GC-MS Analysis and Pharmacological Potential

2023

TJNPR

View full text Add to dashboard Cite

Talinum paniculatum (Talinaceae), also known as Javanese ginseng in Indonesia, is a widely recognized medicinal plant. However, the method of fertilization is currently limited to the leaves, and there needs to be more information about the metabolite profile on its stem. Therefore, this study aimed to identify the content of metabolite present in the stem extract of T. paniculatum and investigate the potential of an introductory class of metabolites to be utilized. To achieve this, the active compounds of the T. paniculatum stem were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) with Ethanol Pro Analysis (99.8%) as organic solvents. The profiles of these compounds were identified using the NCBI International Library PubChem branch, NIST Chemistry WebBook branch, SpectraBase branch of WILEY, and FOODB branch of TMIC. The results showed that T. paniculatum stem extract possesses a dominating compound with antiinflammatory properties.

show abstract

“…The essential oil 200 mg/kg had shown effective result with decrease of WBC, ESR content and increase the Hb, RBC content (Table 6). The chemical constituent, n-hexadecanoic acid is the major constituents present in essential oil results into antiinflammatory effect by inhibition of inflammation mediators such as phospholipase A2, prostaglandins E2, IL-6, IL-1 β, TNFα, and nitric oxide synthase (Aparna et al, 2012;Guerrero et al, 2017;Godara et al, 2019;Joshua et al, 2020).…”

Section: Haematological Parametersmentioning

confidence: 99%

Chemical constituents, antimicrobial and anti-inflammatory activities of Anaphalis triplinervis essential oil

Prakash,

Kaur,

Bala

2023

NVEO

View full text Add to dashboard Cite

The essential oil of Anaphalis triplinervis has been isolated through hydro-distillation and analysed by gas chromatography-mass spectroscopy (GC-MS). The in-vitro antimicrobial activity has been investigated by welldiffusion method against gram-positive and gram-negative bacteria as well as pathogenic fungus and in-vivo antiinflammation activity has been evaluated by using complete Freund's adjuvant (CFA) induced model into the left hind paw oedema. A total of nighteen components have been observed which constitute 93.2% of the total oil, observed major group components are fatty acids (40%), sesquiterpene hydrocarbons (29%), oxygenated sesquiterpenes (19.7%), fatty acids methyl esters (1.1%), diterpene (2.7%), monoterpene (0.8%) and phthalate contamination (0.6%). The main constituents of the oil are n-hexadecanoic acid (33.7%), cis-β-franesene (17.1%), phytone (7.1%), αhumulene (3.8%),elemol (3.6%), caryophyllene oxide (3.4%), δ-cadinene (3.3%) and β-caryophyllene (3%). This essential oil has shown effective antimicrobial activity against both Staphylococcus aureus and Escherichia coli bacteria as well as pathogenic fungus Aspergillus nigerat 400 ppm concentrtion and also has shown the reduction in paw volume and haematological parameters in inflammatory rats at 200 mg/kg dose.

show abstract

Bioassay-guided fractionation, phospholipase A₂-inhibitory activity and structure elucidation of compounds from leaves of Schumanniophyton magnificum

Cited by 12 publications

References 30 publications

Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights

Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights

Phytochemical Profiling of Javanese Ginseng (Talinum paniculatum) Stem Extract Using GC-MS Analysis and Pharmacological Potential

Chemical constituents, antimicrobial and anti-inflammatory activities of Anaphalis triplinervis essential oil

Contact Info

Product

Resources

About

Bioassay-guided fractionation, phospholipase A2-inhibitory activity and structure elucidation of compounds from leaves of Schumanniophyton magnificum

Cited by 12 publications

References 30 publications

Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights

Exploring the Therapeutic Potential of Ammodaucus leucotrichus Seed Extracts: A Multi-Faceted Analysis of Phytochemical Composition, Anti-Inflammatory Efficacy, Predictive Anti-Arthritic Properties, and Molecular Docking Insights

Phytochemical Profiling of Javanese Ginseng (Talinum paniculatum) Stem Extract Using GC-MS Analysis and Pharmacological Potential

Chemical constituents, antimicrobial and anti-inflammatory activities of Anaphalis triplinervis essential oil

Contact Info

Product

Resources

About

Bioassay-guided fractionation, phospholipase A₂-inhibitory activity and structure elucidation of compounds from leaves of Schumanniophyton magnificum