Bioassay-Guided Isolation and Evaluation of Herbal Drugs

Mukherjee, Pulok K.

doi:10.1016/b978-0-12-813374-3.00013-2

Cited by 16 publications

(8 citation statements)

References 136 publications

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“…Cytotoxicity is the primary characteristic of compounds and substances in terms of their qualification as therapeutic agents and is the toxicity that a certain factor causes in live cells [ 76 ]. High cytotoxicity against rapidly dividing cancer cells in vitro is the basis for further research on their bioactivity (e.g., necrosis, autophagy, or apoptosis induction), low cytotoxicity on the other hand is desired in the development of drugs that are not intended to induce death in cells.…”

Section: Biological Activitymentioning

confidence: 99%

Phytochemical Composition and Biological Activities of Scorzonera Species

Lendzion

Gornowicz

Bielawski

et al. 2021

IJMS

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The genus Scorzonera comprises nearly 200 species, naturally occurring in Europe, Asia, and northern parts of Africa. Plants belonging to the Scorzonera genus have been a significant part of folk medicine in Asia, especially China, Mongolia, and Turkey for centuries. Therefore, they have become the subject of research regarding their phytochemical composition and biological activity. The aim of this review is to present and assess the phytochemical composition, and bioactive potential of species within the genus Scorzonera. Studies have shown the presence of many bioactive compounds like triterpenoids, sesquiterpenoids, flavonoids, or caffeic acid and quinic acid derivatives in extracts obtained from aerial and subaerial parts of the plants. The antioxidant and cytotoxic properties have been evaluated, together with the mechanism of anti-inflammatory, analgesic, and hepatoprotective activity. Scorzonera species have also been investigated for their activity against several bacteria and fungi strains. Despite mild cytotoxicity against cancer cell lines in vitro, the bioactive properties in wound healing therapy and the treatment of microbial infections might, in perspective, be the starting point for the research on Scorzonera species as active agents in medical products designed for miscellaneous skin conditions.

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Section: Biological Activitymentioning

confidence: 99%

Phytochemical Composition and Biological Activities of Scorzonera Species

Lendzion

Gornowicz

Bielawski

et al. 2021

IJMS

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“…Cytotoxicity can be defined as the toxicity induced by chemotherapeutic drugs in live cells [157]. The viability of 3D models can be assessed after incubation with the metallodrugs through several methods, such as the alamar blue assay, MTT (3-(4,5-dimethyl-2thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay, CellTiter-Glo ® 3D Viability Assay, or acid phosphatase (APH) assay.…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Cancer 3D Models for Metallodrug Preclinical Testing

Engrácia,

Pinto,

Mendes

2023

IJMS

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Despite being standard tools in research, the application of cellular and animal models in drug development is hindered by several limitations, such as limited translational significance, animal ethics, and inter-species physiological differences. In this regard, 3D cellular models can be presented as a step forward in biomedical research, allowing for mimicking tissue complexity more accurately than traditional 2D models, while also contributing to reducing the use of animal models. In cancer research, 3D models have the potential to replicate the tumor microenvironment, which is a key modulator of cancer cell behavior and drug response. These features make cancer 3D models prime tools for the preclinical study of anti-tumoral drugs, especially considering that there is still a need to develop effective anti-cancer drugs with high selectivity, minimal toxicity, and reduced side effects. Metallodrugs, especially transition-metal-based complexes, have been extensively studied for their therapeutic potential in cancer therapy due to their distinctive properties; however, despite the benefits of 3D models, their application in metallodrug testing is currently limited. Thus, this article reviews some of the most common types of 3D models in cancer research, as well as the application of 3D models in metallodrug preclinical studies.

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“…Although this approach is cost-effective, it generates a lot of incomplete information and necessitates a lot of genetic analysis [170]. According to Mukherjee [171], genotyping-by-sequencing's key strengths are its Despite NGS's advantages, it has several limitations. The requirement for PCR amplification before sequencing is a fundamental limitation of all 2G NGS approaches [166].…”

Section: Genotyping-by-sequencing (Gbs)mentioning

confidence: 99%

Revolutionizing Malaria Vector Control: The Importance of Accurate Species Identification through Enhanced Molecular Capacity

Hadebe,

Malgwi,

Okpeku

2023

Microorganisms

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Many factors, such as the resistance to pesticides and a lack of knowledge of the morphology and molecular structure of malaria vectors, have made it more challenging to eradicate malaria in numerous malaria-endemic areas of the globe. The primary goal of this review is to discuss malaria vector control methods and the significance of identifying species in vector control initiatives. This was accomplished by reviewing methods of molecular identification of malaria vectors and genetic marker classification in relation to their use for species identification. Due to its specificity and consistency, molecular identification is preferred over morphological identification of malaria vectors. Enhanced molecular capacity for species identification will improve mosquito characterization, leading to accurate control strategies/treatment targeting specific mosquito species, and thus will contribute to malaria eradication. It is crucial for disease epidemiology and surveillance to accurately identify the Plasmodium spp. that are causing malaria in patients. The capacity for disease surveillance will be significantly increased by the development of more accurate, precise, automated, and high-throughput diagnostic techniques. In conclusion, although morphological identification is quick and achievable at a reduced cost, molecular identification is preferred for specificity and sensitivity. To achieve the targeted malaria elimination goal, proper identification of vectors using accurate techniques for effective control measures should be prioritized.

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Bioassay-Guided Isolation and Evaluation of Herbal Drugs

Cited by 16 publications

References 136 publications

Phytochemical Composition and Biological Activities of Scorzonera Species

Phytochemical Composition and Biological Activities of Scorzonera Species

Cancer 3D Models for Metallodrug Preclinical Testing

Revolutionizing Malaria Vector Control: The Importance of Accurate Species Identification through Enhanced Molecular Capacity

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