Bioassay-Guided Isolation and HPLC Quantification of Antiproliferative Metabolites from Stahlianthus thorelii

Abstract

In folk medicine, Stahlianthus thorelii Gagnep. has been used to treat diseases related to inflammation, ulcers, and cancer. There are no reports concerning the chemical components and bioactivities of S. thorelii; thus, this study aims to explore the phytochemicals, quantify the main compounds, and test the anticancer activity of isolates from S. thorelii. Dried rhizomes were extracted with 95% ethanol and, then, partitioned, fractionated, and isolated. On the basis of the result of the antiproliferative activity of the fractions, seven isolates were yielded and were identified by spectroscopic analyses. The inhibition of cancer proliferation was determined by an MTT assay and the deployed IC50 to value their efficacy. Seven compounds containing one new C-benzylated dihydrochalcone derivative, thorechalcone A (1) and 2–7 were isolated from S. thorelii. In terms of the bioactivity, compounds 1 and 3 displayed promising antiproliferative activity (WiDr, A549, and HepG2), with IC50 values <40 µM. The HPLC-UV method of quantification of two major compounds (3 and 4) was also validated. This study presented the isolations of antiproliferative potentials of new chalcone and known flavonoid derivatives from S. thorelii. The validated simple, accurate, and rapid HPLC method could be deployed for the quality control of herbal drugs.

Tetrahydroxanthene-1,3(2H)-diones and Oxidized Hexadiene Derivatives from Uvaria leptopoda and Their Biological Activities

“…Among them, three tetrahydroxanthene-1,3(2H)-diones (1−3) and two oxidized hexadiene derivatives (6 and 7) were identified as new compounds. The known compounds found are 9a-O-methyloxymitrone (4), 14 cyathostemmine (5), 15 grandiuvarin C (8), 16 uvamalol G (9), 17 (−)-1,6-desoxytingtanoxide (10), 18 grandiuvarin A (11), 16 valderepoxide (12), 19 grandiuvarin B (13), 16 2′-hydroxy-3′,4′,6′-trimethoxychalcone (14), 20 and macrophyllin (15). 21 Uvarialeptone A (1), HRESIMS m/z 871.2972 [M -H] − (calcd for C 50 H 47 O 14 , 871.2971), was obtained as a colorless needle crystal.…”

Albiflorenes A–L, polyoxygenated cyclohex(a/e)ne diterpene esters from Kaempferia albiflora

“…The nine, previously recognized, compounds isolated from K. albiflora were identified as ent -isopimar-8(14),15-dien-19-oic acid ( 13 ) 21 , dehydroabietic acid ( 14 ) 24 , dehydroabietinol ( 15 ) 31 , 18-norabieta-8,11,13-trien-4-ol ( 16 ) 32 , pomiferin D ( 17 ) 33 , 7-oxodehydroabietinol ( 18 ) 31 , boesenboxide ( 19 ) 34 , (–)-1,6-desoxytingtanoxide ( 20 ) 35 , 36 , and stigmasterol stearate ( 21 ) 37 by comparison of their experimental and reported spectroscopic data.…”