Bioassay-guided isolation and identification of antidiabetic compounds from Garcinia cowa leaf extract

Abstract

Garcinia cowa Roxb. ex Choisy (Clusiaceae) is a Thai local edible plant, which has been used for the treatment of diabetes. The aim of this study is to discover and identify bioactive compounds related to antidiabetic properties from the leaf extract of G. cowa. α-Glucosidase inhibitory bioassay-guided isolation of the ethyl acetate extract of the leaves of G.cowa resulted in the isolation and identification of 11 compounds. Of these, a decahydro-1Hxanthene derivative, garciniacowone K (1), was identified as a novel compound. Their structures were characterized by spectroscopic data and by comparison of their NMR spectroscopic data with those previously reported.All compounds were evaluated for their α-glucosidase inhibitory and glucose consumption activities. Compound 2 showed the highest efficacy in inhibiting α-glucosidase enzyme and promoting glucose consumption activity by 3T3-L1 cells, with IC 50 values of 0.5 μM and 13.1 μM, respectively, without causing toxicity to cells.

Daldiniaeschsone A, a Rare Tricyclic Polyketide Having a Chromone Unit Fused to a δ-Lactone and Its Symmetrical Biphenyl Dimer, Daldiniaeschsone B, from an Endophytic Fungus Daldinia eschscholtzii SDBR-CMUNKC745

“…A colorimetric α -glucosidase assay was carried out by the previously described procedure [ 18 ]. Briefly, the sample solution was dissolved in DMSO after the solutions were diluted with a phosphate buffer (100 mM KH 2 PO 4 , pH 6.9) become DMSO (10%, v/v ) concentration.…”

Polyoxygenated seco-cyclohexenes derivatives from flower and leaf extracts of Desmos cochinchinensis and their α-glucosidase inhibitory activity

“…The information on instruments and materials was the same as previous reports ( Raksat et al., 2019 ; Phukhatmuen et al., 2020 ; Suthiphasilp et al., 2020 ). For more details, please see Supplementary Material.…”

“…A colorimetric α -glucosidase assay was conducted according to the previously described method ( Sharma et al., 2019 ; Phukhatmuen et al., 2020 ; Suthiphasilp et al., 2020 ), using acarbose as a positive control (IC 50 = 83.5 μM).…”

“…The glucose uptake assay was performed according to the method described in the previous reports ( Sharma et al., 2019 ; Phukhatmuen et al., 2020 ; Cheng et al (2006) ) with modification. Metformin (1 mM) and insulin (1 μM) were used as positive controls in the glucose uptake assay with 1.9- and 2.07-fold induction, respectively.…”

A Review on Cytotoxic Activity of (Garcinia Cowa Roxb.): Potential as an Anticancer

“…Apart from cytotoxic activity, the results of previous studies also showed that asam kandis (Garcinia cowa Roxb.) has antidiabetic 5 , antioxidant, antimutagenic 6 , antibacterial 7,8,9 and anti-inflammatory activities 10 . It is hoped that the explanation from this review can increase knowledge and can contribute to the development of anticancer drugs from natural ingredients.…”