Bioassay-guided isolation of anti-adipogenic compounds from defatted pepper (Capsicum annuum L.) seeds

Sung, Jeehye; Bang, Myun‐Ho; Lee, Junsoo

doi:10.1016/j.jff.2015.02.043

Cited by 16 publications

(15 citation statements)

References 16 publications

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“…The representative HPLC chromatograms for CRF and capsicoside G standard are shown Fig. , and ESI‐MS spectra are available in our previous study (Sung et al ., ). The capsicoside G content in CRF was determined from the linear regression equation of the standard calibration curve.…”

Section: Methodsmentioning

confidence: 97%

“…Additionally, pepper seed water extracts inhibited lipoprotein lipase activity in 3T3‐L1 cells (Baek et al ., ). We have previously reported that capsicoside G, which is the major anti‐adipogenic component isolated from pepper seeds, exerts an inhibitory effect on adipocyte differentiation through activation of the 5′‐adenosine monophosphate‐activated protein kinase pathway in 3T3‐L1 cells (Sung et al ., ; Sung and Lee, ). However, the in vivo effects of pepper seeds on HFD‐induced obesity in mice are unknown.…”

Section: Introductionmentioning

confidence: 97%

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Effect of the Capsicoside G‐rich Fraction from Pepper (Capsicum annuum L.) Seeds on High‐fat Diet‐induced Obesity in Mice

Sung

Jeong

2016

Phytotherapy Research

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Obesity is one of the most common metabolic syndromes and is a major threat to human health worldwide. Given the size of this problem, there is growing interest in natural agents that may decrease obesity. In this study, we investigated the anti-obesity effect of a capsicoside G-rich fraction (CRF; 13.35% capsicoside G) isolated from pepper seeds in diet-induced obese mice. C57BL/6J mice were fed either a normal diet or a high-fat diet (HFD), with or without CRF (HFD + CRF; 10 and 100 mg/kg body weight). The body weight and food efficiency ratio of mice fed HFD + CRF were lower in comparison to that of mice fed only an HFD. Epididymal adipose tissue weight and adipocyte hypertrophy were significantly lower in HFD + CRF mice than in HFD mice. The fat deposition in the liver of mice fed HFD + CRF was lower compared to that of mice fed only an HFD. CRF significantly reversed the HFD-induced elevation of the expression of key adipocyte differentiation regulators, including peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, sterol regulatory element binding protein 1c, and their target genes. These results suggest that CRF could be used as dietary therapy for the prevention of obesity and obesity-related metabolic diseases. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Effect of the Capsicoside G‐rich Fraction from Pepper (Capsicum annuum L.) Seeds on High‐fat Diet‐induced Obesity in Mice

Sung

Jeong

2016

Phytotherapy Research

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“…Capsicoside G was isolated from pepper ( Capsicum annuum L.) seeds and characterised as previously described (Sung, Bang, & Lee, ). Bovine calf serum, foetal bovine serum (FBS), high‐glucose Dulbecco’s modified Eagle’s medium (DMEM), phosphate‐buffered saline (PBS), Oil red O and penicillin‐streptomycin were purchased from Gibco (Grand Island, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Capsicoside G was isolated from pepper (Capsicum annuum L.) seeds and characterised as previously described (Sung, Bang, & Lee, 2015…”

Section: Materials and Reagentsmentioning

confidence: 99%

“…We previously reported that capsicoside G, a furostanol saponin isolated from pepper seeds, has inhibitory effects on lipid accumulation and adipogenesis by activating AMP‐activated protein kinase in 3T3‐L1 cells, thus suggesting their anti‐obesity potential (Sung, & Lee, ; Sung, Bang, & Lee, ). The purpose of the present study was to extend the anti‐obesity investigation of capsicoside G by examining its effects on lipolysis and fatty acid oxidation in mature 3T3‐L1 adipocytes.…”

Section: Introductionmentioning

confidence: 99%

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Effects of capsicoside G isolated from pepper seeds on lipolysis and fatty acid oxidation‐related gene expression in 3T3‐L1 adipocytes

2018

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We previously demonstrated that capsicoside G, a furostanol saponin isolated from pepper seeds, inhibits adipogenesis in 3T3‐L1 adipocytes. However, the effects of capsicoside G on lipolysis and fatty acid oxidation remain unclear. The purpose of this study was to extend the anti‐obesity investigation of capsicoside G by examining its effect on lipolysis and fatty acid oxidation‐related gene expression in mature 3T3‐L1 adipocytes. Capsicoside G promoted lipolysis of adipocytes, which effectively decreased the intracellular triglyceride concentration of adipocytes and increased the amount of glycerol released into the medium. Increased lipolysis was accompanied by augmented expression of adipose triglyceride lipase and hormone‐sensitive lipase. Furthermore, capsicoside G induced the expression of fatty acid oxidative genes such as peroxisome proliferator‐activated receptor α and carnitine palmitoyltransferase‐1 in mature 3T3‐L1 adipocytes. These results suggest that capsicoside G exerts anti‐obesity effects by stimulating lipolysis and fatty acid oxidation‐related gene expression in mature 3T3‐L1 adipocytes. Practical application Capsicoside G, a furostanol saponin isolated from pepper seeds, has been reported to have inhibitory effects on lipid accumulation and adipogenesis by activating AMP‐activated protein kinase in 3T3‐L1 cells, thus suggesting their anti‐obesity potential. In this study, capsicoside G stimulated lipolysis and fatty acid oxidation‐related gene expression in mature 3T3‐L1 adipocytes. Therefore, our findings suggest that capsicoside G is a promising therapeutic tool for obesity and obesity‐related metabolic syndromes.

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Bioassay-guided isolation of anti-adipogenic compounds from defatted pepper (Capsicum annuum L.) seeds

Cited by 16 publications

References 16 publications

Effect of the Capsicoside G‐rich Fraction from Pepper (Capsicum annuum L.) Seeds on High‐fat Diet‐induced Obesity in Mice

Effect of the Capsicoside G‐rich Fraction from Pepper (Capsicum annuum L.) Seeds on High‐fat Diet‐induced Obesity in Mice

Effects of capsicoside G isolated from pepper seeds on lipolysis and fatty acid oxidation‐related gene expression in 3T3‐L1 adipocytes

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