Bioassay‐Guided Isolation of Antifungal Compounds from Disporopsis aspersa (<scp>Hua</scp>) <scp>Engl</scp>. ex <scp>Diels</scp> against Pseudoperonospora cubensis and Phytophthora infestans

Zhu, Feng; Yuan, Chengqian; Gang, Fangli; Yang, Chao; Wu, Wenjun; Zhang, Jiwen

doi:10.1002/cbdv.201800090

Cited by 14 publications

(10 citation statements)

References 29 publications

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“…Disporopsis aspera is a perennial herb of Disporopsis genus of Liliaceae, and its rhizomes are used as medicine in traditional remedies, having the functions of easing thirst, nourishing yin and moistening. In our previous work, we first reported that crude extracts from D. aspera exhibited excellent antifungal activity, and isolated l ‐pyroglutamic acid has preferable control effects on Pseudoperonospora cubensis and Phytophthora infestans 18 . In this study, we found that the crude extract of the rhizomes of D. aspera exhibited effective antifungal activity against Podosphaera xanthii .…”

Section: Introductionmentioning

confidence: 64%

“…Our prior work revealed that L-pyroglutamic acid and salicylic acid isolated from D. aspera showed promising antifungal activity against Phytophthora infestans and Pseudoperonospora cubensis. 18 In this work, we reported that D. aspera rhizomes extract has excellent inhibitory activity against Podosphaera xanthii and L-Aze was isolated and identified as the main active substance from the extract of D. aspera rhizome.…”

Section: Discussionmentioning

confidence: 94%

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Antifungal activity of l‐azetidine‐2‐carboxylic acid isolated from Disporopsis aspera rhizomes against Podosphaera xanthii

Zhao

Zhou

et al. 2022

Pest Management Science

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BACKGROUND Powdery mildew is one of the fungal diseases commonly occurring in the process of cucurbits protected and open cultivation. Cucumbers, melons and pumpkins are extremely susceptible. The secondary metabolites produced by plants are important sources of fungicides with low toxicity and environment‐friendly characteristics. The aim of this study was to reveal the main active ingredient in the crude extracts of Disporopsis aspera rhizomes that inhibit cucurbits powdery mildew and evaluate its activities. RESULTS In this study, the crude extracts of Disporopsis aspera rhizomes were found to exhibit excellent antifungal activity aganist Podosphaera xanthii, a causal agent of cucurbits powdery mildew. Based on the bioassay‐guided method, l‐azetidine‐2‐carboxylic acid (l‐Aze) was isolated from this genus for the first time. l‐Aze showed unique curative and eradicative activity against Podosphaera xanthii in vivo, which has never been reported before. Microscopic observation revealed that the curative spraying of l‐Aze could effectively inhibit the mycelial growth, resulting in hollow parts of the mycelia, not forming conidiophores, and interrupting the life cycle of powdery mildew. The eradicative spraying of l‐Aze caused the fracture of mycelia and deformity of conidiophores, which could not continue to produce conidia. CONCLUSION l‐Aze was the main active ingredient of D. aspera against Podosphaera xanthii, which had both curative and eradicative effects. The results provided a strong possibility of using the crude extracts of D. aspera rhizomes and its main effective component, l‐Aze as biocontrol agents to control cucurbits powdery mildew.

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Section: Introductionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 94%

Antifungal activity of l‐azetidine‐2‐carboxylic acid isolated from Disporopsis aspera rhizomes against Podosphaera xanthii

Zhao

Zhou

et al. 2022

Pest Management Science

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“…Previous studies have shown that purified pyroglutamic acid exhibits antibacterial properties against Bacillus and Enterobacter strains, as well as antifungal activity against phytopathogens (Huttunen, Noro, & Yang, 1995;F. Zhu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Pyroglutamic acid is a potential precursor of glutamate, which is essential for the first step in the de novo biosynthesis of the antioxidant glutathione (Forman, Zhang, & Rinna, 2009; Kumar & Bachhawat, 2012). Previous studies have shown that purified pyroglutamic acid exhibits antibacterial properties against Bacillus and Enterobacter strains, as well as antifungal activity against phytopathogens (Huttunen, Noro, & Yang, 1995; F. Zhu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Characterization of epidermal bladder cells in Chenopodium quinoa

Otterbach

Khoury

Rupasinghe

et al. 2021

Plant Cell & Environment

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Chenopodium quinoa (quinoa) is considered a superfood, as it has favourable nutrient composition and is gluten free. Quinoa has high tolerance to several abiotic stresses, i.e. salinity, water deficit (drought) and cold. The tolerance mechanisms are yet to be elucidated. Quinoa has Epidermal Bladder Cells (EBCs) that densely cover the shoot surface, particularly the younger parts of the plant. Here, we report on the EBC's primary and secondary metabolomes, as well as the lipidome in response to abiotic stresses. EBCs were isolated from plants after cold, heat, high-light, water deficit and salt treatments. We used untargeted Gas Chromatography-Mass Spectrometry (GC-MS) to analyse metabolites and untargeted and targeted Liquid Chromatography-MS (LC-MS) for lipids and secondary metabolite analyses. We identified 64 primary metabolites, including sugars, organic acids and amino acids, 19 secondary metabolites, including phenolic compounds, betanin and saponins and 240 lipids categorized in five groups including glycerolipids and phospholipids. Although we found only few changes in the metabolic composition of bladders in response to abiotic stresses, metabolites related with heat, cold and high-light treatments, but not salt stress, were changed significantly. Na + concentrations were low in EBCs with all treatments, and approximately two orders of magnitude lower than K + concentrations.

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“…The latter is known as a powerful antioxidant [33]. Previous studies have shown that l -pyroglutamic acid exhibits both antibacterial [34] and antifungal activity against phytopathogenic Pseudoperonospora cubensis and Phytophthora infestans [35]. To the best of our knowledge, there are no reports characterizing the impact of this compound on Fusaria.…”

Section: Introductionmentioning

confidence: 99%

Resistance-Related l-Pyroglutamic Acid Affects the Biosynthesis of Trichothecenes and Phenylpropanoids by F. graminearum Sensu Stricto

Bilska

Stuper‐Szablewska

Kulik

et al. 2018

Toxins

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Fungicide application remains amongst the most widely used methods of fungal control in agroecosystems. However, the extensive use of fungicides poses hazards to human health and the natural environment and does not always ensure the effective decrease of mycotoxins in food and feed. Nowadays, the rising threat from mycotoxin contamination of staple foods has stimulated efforts in developing alternative strategies to control plant pathogenic fungi. A substantial effort is focused on the identification of plant-derived compounds inhibiting mycotoxin production by plant pathogenic fungi. l-Pyroglutamic acid has recently been suggested as playing a role in the response of barley to toxigenic Fusaria. Considering the above, we studied the response of various strains of F. graminearum sensu stricto to different levels of l-pyroglutamic acid on solid YES (yeast extract sucrose) media. l-Pyroglutamic acid decreased the accumulation of trichothecenes in all examined strains. Gene expression studies addressing Tri genes (Tri4, Tri5, and Tri10), which induce the biosynthesis of trichothecenes, revealed the production of mycotoxins by l-pyroglutamic acid to be inhibited at the transcriptional level. Besides inhibitory effects on mycotoxin production, l-pyroglutamic acid exhibited variable and concentration-related effects on phenylpropanoid production by fungi. Accumulation of most of the fungal-derived phenolic acids decreased in the presence of 100 and 400 µg/g of l-pyroglutamic acid. However, a higher dose (800 µg/g) of l-pyroglutamic acid increased the accumulation of trans-cinnamic acid in the media. The accumulation of fungal-derived naringenin increased in the presence of l-pyroglutamic acid. Contrasting results were obtained for quercetin, apigenin, luteolin, and kaempferol, the accumulation of which decreased in the samples treated with 100 and 400 µg/g of l-pyroglutamic acid, whereas the highest l-pyroglutamic acid concentration (800 µg/g) seemed to induce their biosynthesis. The results obtained in this study provide new insights for breeders involved in studies on resistance against Fusaria.

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Bioassay‐Guided Isolation of Antifungal Compounds from Disporopsis aspersa (Hua) Engl. ex Diels against Pseudoperonospora cubensis and Phytophthora infestans

Cited by 14 publications

References 29 publications

Antifungal activity of l‐azetidine‐2‐carboxylic acid isolated from Disporopsis aspera rhizomes against Podosphaera xanthii

Antifungal activity of l‐azetidine‐2‐carboxylic acid isolated from Disporopsis aspera rhizomes against Podosphaera xanthii

Characterization of epidermal bladder cells in Chenopodium quinoa

Resistance-Related l-Pyroglutamic Acid Affects the Biosynthesis of Trichothecenes and Phenylpropanoids by F. graminearum Sensu Stricto

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