Bioassay-Guided Isolation of Antioxidants from Diospyros lotus Roots

Uddin, Ghias; Jehan, Noor; Ahmad, Zarka; Ramadan, Mohamed Fawzy; Khan, Haroon; Bahadar, Alia; Hadda, Taïbi Ben; Ali, Mumtaz

doi:10.1007/s10600-017-2164-3

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…The shade-dried leaves, fruit, and stem bark of E. serrata and E. obtusifolia were powdered and then extracted with 99% methanol by heating under reflux. The filtration of methanolic extract was carried out and the conversion of filtrate to a semisolid mass was brought about by means of rotary evaporation according to the method reported [15,16]. For in vivo findings, the extracts were administered orally after mixing them in normal saline.…”

Section: Preparation Of Methanol Extractmentioning

confidence: 99%

Evaluation of antinociceptive potential of methanolic extract of different parts of Ehretia serrata Roxb and Ehretia obtusifolia in vivo

Zill-e-Huma¹,

Rauf²,

Natasha³

et al. 2018

biomedicalresearch

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Background: The antinociceptive potential study of different parts of Ehretia serrata Roxb and Ehretia obtusifolia are least explored till now. These plant parts are usually used as fodder and wood in Asia. The aim of this work was to find the antinociceptive effects of the methanolic extracts of leaves, fruits and stem barks of these plants in mice. Methods: The antinoceceptive activity of methanolic extract of leaf, fruit, and stem bark of E. serrata and E. obtusifolia in mice was carried out by means of the hot plate method and by using diclofenac sodium as a standard. Twenty groups of 12 h starved mice were prepared where each group comprised of five mice only. While all these starved mice were allowed free access to clean water. In this study, both male and female Albino mice were used. Analgesiometer was used for this bio-essay. Results: The results showed that the dose significantly (P<0.05) reduced the time spent in pain behaviour in all assessment times (0 min, 30 min and 60 min) hence indicating that the plant possesses antinociceptive potential. Conclusion: It is confirmed through the findings that E. serrata and E. obtusifloia exhibit strong antinociceptive action in animal model of hot plate which needs verification in other paradigms too.

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Section: Preparation Of Methanol Extractmentioning

confidence: 99%

Evaluation of antinociceptive potential of methanolic extract of different parts of Ehretia serrata Roxb and Ehretia obtusifolia in vivo

Zill-e-Huma¹,

Rauf²,

Natasha³

et al. 2018

biomedicalresearch

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“…Phytochemical investigation of Diospyros lotus indicated the presence of dimeric naphthoquinones, steroids, naphthalene derivatives, and lupine triterpenes. − The phytochemical studies of the fruits indicated the presence of fatty acids, nonvolatile acids, phenolic groups, and sugars . Phytochemicals such as diospyrin and 8-hydroxdiospyrin have been reported for their excellent analgesic, sedative, and anti-inflammatory effects. , The recently reported dinaphthodiospyrol (A–H) has been reported for analgesic, sedative, muscle relaxation, antiulcer and anticancer properties. − In this work, we try to understand and determine the antioxidant and antimicrobial activities of the dimeric naphthoquinones ( 1–7 ) purified from Diospyros lotus against infectious diseases.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial, Antifungal, Antioxidant, and Docking Studies of Potential Dinaphthodiospyrols from Diospyros lotus Linn Roots

Johny

Anwar

Naz³

et al. 2021

ACS Omega

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The main aims of this investigation were the isolation of dimeric naphthoquinones, a new class of dinaphthodiospyrols (1–7), from chloroform fractions and screening them for antibacterial, antifungal, and antioxidant potential. The susceptibility of the isolated compounds, namely, dinaphthodiospyrol A (1), dinaphthodiospyrol B (2), dinaphthodiospyrol C (3), dinaphthodiospyrol D (4), dinaphthodiospyrol E (5), dinaphthodiospyrol F (6), and dinaphthodiospyrol G (7) was assessed for antibacterial potential using well diffusion methods. The isolated compounds showed excellent antibacterial activity against selected bacterial strains, including Gram-positive Bacillus subtilis, Streptococcus epidermis, and Bacillus subtilis, and Gram-negative bacteria Klebsiella pneumonia with the zones of inhibition 6 to 26 nm. The standard drug Imipenem showed a maximum inhibitory zone 30 to 35 nm. Similarly, the isolated compounds were screened for antifungal properties, which showed an excellent reduction in the growth of selected fungal strain including Candida albicans, Aspergillus flavus, Fusarium solani, Trichyton logifusus, Microsporum canis , and Candida glabrata. Among all the screened compounds, 7 exhibited good activity (30–49 mm), followed by compounds 5 and 6, (35–46 mm), while compounds 1–4 showed a moderate effect (8–28 mm) against the selected fungal strain against miconazole which showed potent effects (101–110.98 mm). The isolated compounds were also screened for 1, 1-diphenyl-2-picrylhydrazyl (DPPH) activity. In vitro-based free radical was employed using ascorbic acid as a standard antioxidant. The tested compounds (1–7) exhibited significant antioxidant activity in a concentration-dependent manner. The dinaphthodiospyrol 7 exhibited 97.32% scavenging activity, followed by dinaphthodiospyrol 6, 92.01%, and compounds 5 and 4 with 89.90 and 88.43% scavenging activity at 100 μg/mL, respectively; ascorbic acid showed 96.45% scavenging effect. Furthermore, docking analysis was performed to know the exact binding mode of the tetra-substituted derivatives of dinaphthodiospyrols to the selected target proteins. From the docking analysis, it was found that the docking results are well correlated with the experimental observations. In conclusion, the dinaphthodiospyrols exhibited excellent antibacterial, antifungal, and free radical scavenging potential.

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“…ssp. nucifera) contains high phenolic compounds, which are potential free radical scavengers and antioxidants (Rauf et al, ). Recently, fresh‐cut lotus root commercialized gradually due to its rich nutrient and the crisp organization, which is suitable for the fresh‐cut processing.…”

Section: Introductionmentioning

confidence: 99%

Effect of konjac glucomannan coating on antioxidant capacity and phenolic metabolism in fresh-cut lotus roots

Zhou

Huang

2018

J Food Process Preserv

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Konjac glucomannan (KGM) was used as a natural coating matrix applied on fresh‐cut lotus roots at 4°C during 8 days storage. Treatment with 20 g/L KGM significantly maintained the color (L value), inhibited the respiration rate and delayed the decrease of the soluble solid content of lotus roots. For the antioxidant capacity test, KGM can enhance the antioxidant capacity by increasing activities of catalase and superoxide dismutase and increase the antioxidant capacity. Similar to the antioxidant capacity, the total phenolic contents in treated groups also exhibited higher value than untreated ones. Also, konjac glucomannan inhibited the degradation of catechol and increased cinnamic acid contents. In relation with phenolic metabolism, KGM at 20 g/L can improve the activities of peroxidase and phenylalanine ammonia lyase, but inhibit the activity of polyphenol oxidase. These observations indicted that KGM could maintain the quality of fresh‐cut lotus root by the phenolic metabolism and antioxidant capacity. Practical applications This study suggested that konjac glucomannan inhibited tissue browning and maintain qualities of fresh‐cut lotus roots. This study is also helpful to understand the regulation of tissue browning in fresh‐cut lotus roots treated by konjac glucomannan. We have also explored the mechanism of the inhibition of tissue browning in lotus root slices coated with konjac glucomannan by the pathways of reactive oxygens and phenols. Concerned the nontoxic character and the high efficiency to preserve lotus root slices, konjac glucomannan can be a promising potential treatment to preserve qualities of fresh‐cut fruits and vegetables, and this natural product could be considered as good candidates to be commercial preservations.

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Bioassay-Guided Isolation of Antioxidants from Diospyros lotus Roots

Cited by 6 publications

References 8 publications

Evaluation of antinociceptive potential of methanolic extract of different parts of Ehretia serrata Roxb and Ehretia obtusifolia in vivo

Evaluation of antinociceptive potential of methanolic extract of different parts of Ehretia serrata Roxb and Ehretia obtusifolia in vivo

Antibacterial, Antifungal, Antioxidant, and Docking Studies of Potential Dinaphthodiospyrols from Diospyros lotus Linn Roots

Effect of konjac glucomannan coating on antioxidant capacity and phenolic metabolism in fresh-cut lotus roots

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