Bioassay-Guided Isolation of Iridoids and Phenylpropanoids from Aerial Parts of Lamium album and Their Anti-inflammatory Activity in Human Neutrophils

Abstract

In traditional medicine, flowers and aerial parts of are assigned by their anti-inflammatory, antiseptic, and mucolytic activities, and are used in chronic bronchitis and pharyngitis as well as skin, vaginal, and cervical inflammation.The aim of the present study was to compare effects of ethanolic extracts prepared from flowers and aerial parts of on selected functions of human neutrophils, which are involved in an inflammatory response. In order to identify the compounds engaged in the anti-inflammatory activity of extracts, the bioassay-guided isolation of compounds was performed based on the inhibition of cytokine secretion by stimulated neutrophils.The extracts were phytochemically characterized with the HPLC-DAD-MS method. The inhibition of reactive oxygen species production by formyl-met-leu-phenylalanine- or phorbol 12-myristate 13-acetate-stimulated neutrophils was determined using luminol- or lucigenin-dependent chemiluminescence. The effect on myeloperoxidase secretion by neutrophils was established spectrophotometrically. The levels of cytokine (interleukin 8, TNF-) production after extract treatment was measured by ELISA.The most abundant constituents of extracts were phenylpropanoids, iridoids, flavonoids, and phenolic acids. Both extracts at concentrations of 25 and 100 µg/mL significantly inhibited reactive oxygen species production, and myeloperoxidase and interleukin 8 secretion. The phenylethanoid glycosides, such as lamiusides A, B, and C as well as 6″-O--D-glucopyranosylmartynoside, were isolated and identified. The cells treated with 6″---D-glucopyranosylmartynoside and lamiuside B produced 29.47 ± 7.11 % and 64.67 ± 5.25 % of interleukin 8, respectively, compared to non-treated control cells.Our results support the traditional use of and indicate it as a potential source of natural anti-inflammatory constituents, such as phenylpropanoids.

A Review on the Dietary Flavonoid Tiliroside

“…Further investigations indicated that, although tiliroside inhibits the phosphorylation of MAPK/JNK and MAPK/p38 proteins, it still demonstrates no significant inhibition of the phosphorylation of MAPK/ERK or the degradation of IκB‐α (Jin et al., ). Czerwińska, Świerczewska, Woźniak, and Kiss () reported that tiliroside at a concentration of 25 μM could effectively inhibit IL‐8, TNF‐α, and ROS generation in polymorphonuclear leukocytes; however, this activity was lower than those of the reference compounds dexamethasone and oleocanthal.…”

Glandular trichomes, essential oil composition, anti-aspergillus and antioxidative activities of Lamium purpureum L. ethanolic extracts

“…Furthermore, the R 2 values obtained between the total phenol content and ABTS assay, as well as between flavonoids and the ABTS were 1.00 and 0.987, respectively. [1,2,5,6,[9][10][11] 13.83% Oxygenated monoterpenes [14,[17][18][19] 6.99% Sesquiterpene hydrocarbons [25,27,29,30,33,34,35] 6.36%…”

“…These species exhibit multiple biological actions, including antimicrobial [6], antifungal [7], antioxidant [8][9][10][11][12], antiinflammatory and nociceptive [13] as well as anticancer [14,15] activities. Literature data point to the presence of different classes of chemical compounds: including ecdysone and phenylpropanoid glucosides [16], iridoid glucosides [17,18] and oligosaccharides [19]. Interest in the medicinal properties of these plants has led to intensive phytochemical and pharmacological investigations [5,20,21].…”

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“…In Spain its root is used in wound healing and its flower to eliminate severe diarrhea. Studies on the L. album aerial parts extract in order to evaluate its pharmacological properties have been done and showed the following properties in different condition: purify the blood, lowers blood sugar, treats anemia and mild diarrhea, stomachulcers, kidneystones, rheumatism, varicoseveins, sore, insomnia, dandruff, depression, regulate menstruation, induce hair growth, reduce joints pain and detoxifies the body 5 . Other effects are in preventing menstrual disorders, abdominal inflammation, musculoskeletal diseases 15 antioxidant properties 16 .…”

“…Superoxide have useful role in regulating cell growth and intercellular messages. Plants with antioxidant properties can also have anti-inflammatory, anti-depressant, and so on effects 5 .…”