Bioassay-guided isolation of Poliovirus-inhibiting constituents from<i>Zephyranthes candida</i>

Ogbole, Omonike O.; Oriabure, Ajaiyeoba Edith; Adekunle, Adeniji Johnson; Ramsay, Kamdem Soup T; Shyyaula, Sajan; Choudhary, M. Iqbal

doi:10.3109/13880209.2014.946061

Cited by 22 publications

(14 citation statements)

References 18 publications

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“…The leaf extract of Macaranga barteri demonstrated the highest cytotoxic activity (CC 50 = 0.27 μg/mL), antiviral activity (IC 50 = 0.028 ng/mL on E7; 0.00167 ng/mL on E19) and selectivity index (SI = 7971 on E7; 130,177.50 on E19). It was observed that the cytotoxicity evaluation of the extracts seemed to correspond directly to their anti-echovirus activities, a finding similar to our earlier result [ 15 ]. Ipomoea asarifolia leaf extract also showed potent antiviral activity against E7 (IC 50 = 0.0025 μg/mL) with a high selectivity index (SI = 35,004) but was inactive against E19.…”

Section: Discussionsupporting

confidence: 91%

“…Also, a wide range of flavonoids has been shown to possess antiviral activities against a variety of RNA viruses such as poliovirus, sindbis virus, respiratory syncytial virus (RSV), and DNA virus such as herpes simplex virus (HSV) [ 21 ]. The proposed antiviral mechanisms of action of flavonoids include inhibition of viral polymerase and binding of viral nucleic acid or viral capsid proteins [ 15 ]. Previous HPLC profiling of the DCM extract of M. barteri leaves revealed 3,5-dicaffeoylquinic acid, acteoside, kampferol-7- O -glucoside and bastadin-11 as its major constituents [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…The relatively high selectivity of the M. barteri extract and fractions, especially the most active sub-fraction (SI = 274.70 on E7; 246.10 on E19) could be attributed to minimal toxicity possessed by the supposedly constituted flavonoids since flavonoids are believed to possess minimal toxicity, being distributed in edible plants and beverages [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

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In vitro antiviral activity of twenty-seven medicinal plant extracts from Southwest Nigeria against three serotypes of echoviruses

Ogbole

Akinleye

Segun

et al. 2018

Virol J

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BackgroundEchoviruses, a serotype of enteroviruses, infect millions of people globally and there is no specific drug treatment or vaccine available for its management. The screening of medicinal plants used locally for the treatment of infectious diseases, can provide a reliable option in the discovery of potent therapeutic compounds. This study was carried out to investigate the antiviral activities of 27 medicinal plant extracts, belonging to 26 different plant species, selected from Nigerian ethnobotany, against echovirus 7, 13 and 19 serotypes (E7, E13 and E19, respectively).MethodsThe plants were macerated in methanol and the cytotoxicities of the crude extracts were evaluated on the rhabdomyosarcoma cell line using the MTT assay. The antiviral activity of the plant extracts and fractions against echoviruses (E7, E13, and E19) was determined using the neutralisation assay, an assay that measures the inhibition of cytopathic effect on cell culture.ResultsThe crude extract of Macaranga barteri leaves had the highest cytotoxicity with CC50 value of 0.27 μg/mL. This was followed by Crinum jagus (9.88 μg/mL) and Terminalia ivorensis (12.14 μg/mL). The antiviral screening showed that ten out of the 27 crude plant extracts tested were active on E7 and E19, inhibiting the cytopathic effect of the virus in tissue culture. None of the extracts inhibited the cytopathic effect caused by E13 serotype. Amongst the active plant extracts, the methanol extract of M. barteri leaves had the highest antiviral activity on both E7 and E9 with IC50 values of 0.028 and 0.0017 ng/mL, respectively, followed by the Ageratum conyzoides extract (0.208 μg/mL, E7; 0.006 μg/mL, E19) and Mondia whitei extract (0.038 μg/mL, E7; 0.005 μg/mL, E19). Amongst the fractions of M. barteri, the DCM fraction was most the active and selective on E7 (IC50 = 0.0075 ng/mL; SI = 19,896.54) and E19 (IC50 = 0.0175 ng/mL; SI = 8581.24).ConclusionOur research has demonstrated that Macaranga barteri extracts has potent antiviral activity against echoviruses E7 and E19, and our findings suggest that this extract may have potential as a therapeutic agent in the treatment of enteroviral infections.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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In vitro antiviral activity of twenty-seven medicinal plant extracts from Southwest Nigeria against three serotypes of echoviruses

Ogbole

Akinleye

Segun

et al. 2018

Virol J

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“…To determine whether the fractions will be cytotoxic to cancer cell lines with very minimal toxicity to normal cells, the selectivity index (SI) of the most active fraction was determined. For in vitro studies, SI value less than 1 is classified as non-selective (toxic), between 1 and 10 is weakly selective and a SI higher than 10 is considered safe (non-toxic) [ 26 , 27 ]. From the selectivity studies, DMB had a SI value of 13.7 when compared to CTX with SI of 5.4 (Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxic activity of medicinal plants from Nigeria ethnomedicine on Rhabdomyosarcoma cancer cell line and HPLC analysis of active extracts

Ogbole

Segun

2017

BMC Complement Altern Med

118

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BackgroundCancer is a leading cause of death world-wide, with approximately 17.5 million new cases and 8.7 million cancer related deaths in 2015. The problems of poor selectivity and severe side effects of the available anticancer drugs, have demanded the need for the development of safer and more effective chemotherapeutic agents. The present study was aimed at determining the cytotoxicities of 31 medicinal plants extracts, used in Nigerian ethnomedicine for the treatment of cancer.MethodsThe plant extracts were screened for cytotoxicity, using the brine shrimp lethality assay (BSLA) and MTT cytotoxicity assay. Rhabdomyosarcoma (RD) cell line, normal Vero cell line and the normal prostate (PNT2) cell line were used for the MTT assay, while Artemia salina nauplii was used for the BSLA. The phytochemical composition of the active plant extracts was determined by high performance liquid chromatography (HPLC) analysis.ResultsThe extract of Eluesine indica (L.) Gaertn (Poaceae), with a LC50 value of 76.3 μg/mL, had the highest cytotoxicity on the brine shrimp larvae compared to cyclophosphamide (LC50 = 101.3 μg/mL). Two plants extracts, Macaranga barteri Mull. Arg. (Euphorbiaceae) and Calliandra portoricensis (Jacq.) Benth (Leguminosae) exhibited significant cytotoxic activity against the RD cell line and had comparable lethal activity on the brine shrimps. Further cytotoxic investigation showed that the dichloromethane fraction of Macaranga barteri (DMB) and the ethyl acetate fraction of Calliandra portoricensis (ECP), exhibited approximately 6-fold and 4-fold activity, respectively, compared to cyclophosphamide on RD cell line. Determination of selective index (SI) using Vero and PNT2 cell line indicated that DMB and ECP displayed a high degree of selectivity against the cancer cell under investigation. HPLC analysis showed that 3,5dicaffeoylquinic acid, acteoside, kampferol-7-O-glucoside and bastadin 11 were the major components of DMB while the major components of ECP were neurolenin B, nigrosporolide and trans-geranic acid.ConclusionThe results demonstrate the cytotoxicity of Macaranga barteri and Calliandra portoricensis extracts, which are used in Nigerian folklore for cancer treatment.

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“…However lycorine was most active as compared to trisphaeridine and 7-hydroxy-3',4'-methylenedioxyflavan [51,52].…”

Section: Antiviral Activitymentioning

confidence: 99%

Phytochemistry and Pharmacology of Genus Zephyranthes

Katoch¹,

Singh²

2015

Med Aromat Plants

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Bioassay-guided isolation of Poliovirus-inhibiting constituents fromZephyranthes candida

Abstract: The antipoliovirus activity of trisphaeridine (2) and 7-hydroxy-3',4'-methylenedioxyflavan (3) is established in this report; these compounds are of moderate toxicity and have very good SI. They could be a potential template for the development of a new antiviral agent.

Cited by 22 publications

References 18 publications

In vitro antiviral activity of twenty-seven medicinal plant extracts from Southwest Nigeria against three serotypes of echoviruses

In vitro antiviral activity of twenty-seven medicinal plant extracts from Southwest Nigeria against three serotypes of echoviruses

In vitro cytotoxic activity of medicinal plants from Nigeria ethnomedicine on Rhabdomyosarcoma cancer cell line and HPLC analysis of active extracts

Phytochemistry and Pharmacology of Genus Zephyranthes

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