Bioassay guided screening, optimization and characterization of antioxidant compounds from high altitude wild edible plants of Ladakh

Avasthi, Anupama Sharma; Bhatnagar, M; Sarkar, Nandan; Kitchlu, Surinder; Ghosal, Shibnath

doi:10.1007/s13197-016-2300-2

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…A.C, this study shows that Tephrosia species have acceptable levels of ash content as natural food products. Wild edible plants high in carbohydrate are helpful for the body to meet up with daily activities, and most of them contains antioxidant compounds [43]. In this plant high carbohydrate content are more advantageous than those with excess protein because the body does not require too much of protein and fat.…”

Section: Discussionmentioning

confidence: 99%

Medicinally active principles analysis of Tephrosia apollinea (Delile) DC. growing in the United Arab Emirates

et al. 2017

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Background Tephrosia apollinea is a leguminous plant and is native to southwest Asia, Arabia, northwestern India and northeast Africa. In traditional system, it is used for medicinal and coloring purpose. The present study aims to explore the phytochemical, proximate analysis, element contents and antioxidant potential of T. apollinea extract.MethodsThe phytochemical screening was done with qualitative methods. Proximate analysis and elemental composition were performed from powdered sample. In vitro antioxidant assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and reducing power-scavenging assays were used for evaluating the antioxidant properties.ResultsQualitative screening of methanolic extract of T. apollinea showed the presence of alkaloids, phenolics, flavonoids, terpenoids, glycosides and saponins. The methanolic extract of T. apollinea exhibited a significant dose dependent inhibition of DPPH activity, with a 50% inhibition (IC50) at a concentration found to be 29.41 µg/ml, which was compared with standard GAE (IC50 = 31.09 μg/ml). The reducing power shows good linear relationship in both standard gallic acid (R2 = 0.956) and T. apollinea extract (0.984).ConclusionsThe results of our study clearly suggested that the methanolic extract of T. apollinea may serve as potential source of natural antioxidant for nutraceutical application.

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Section: Discussionmentioning

confidence: 99%

Medicinally active principles analysis of Tephrosia apollinea (Delile) DC. growing in the United Arab Emirates

et al. 2017

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“…Compound methyl rosmarinate was identified at m/z 374, as previously reported in E. tinifolia ethyl acetate portion [26], and was known for its antioxidant and antifungal activities [22,27]. For the identification of the remaining compounds, the m/z values of the molecular ion [M-H] − and [M + HCOO] − were compared with the Waters Unifi Software Traditional Medicine Library (Waters Corporation, Milford, MA, USA) and corroborated from the previously reported literature [28][29][30][31][32][33][34][35][36][37][38][39][40][41][42]. Among these compounds, schisantherin A, kaempferol-3,7diglucoside, procyanidin A2, and tocopherol exert anti-inflammatory and antioxidant activity and promote reproductive functions [43][44][45][46], which explain the potent antioxidant and anti-inflammatory activity observed in E. tinifolia.…”

Section: Rosmarinic Acid Is a Major Consituent Of Etmementioning

confidence: 59%

Inhibitory Effects of Ehretia tinifolia Extract on the Excessive Oxidative and Inflammatory Responses in Lipopolysaccharide-Stimulated Mouse Kupffer Cells

Lim,

Lee,

Yun

et al. 2023

Antioxidants

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Ehretia tinifolia (E. tinifolia) L., an evergreen tree with substantial biological activity, including antioxidant and anti-inflammatory effects, has been used in many herbal and traditional medicines. To elucidate its antioxidant and anti-inflammatory activity and the underlying mechanisms, we applied a methanol extract of E. tinifolia (ETME) to lipopolysaccharide (LPS)-stimulated mouse immortalized Kupffer cells. ETME suppressed the LPS-induced increase in nitric oxide, a mediator for oxidative stress and inflammation, and restored LPS-mediated depletion of total glutathione level by stabilizing antioxidative nuclear factor erythroid 2-related factor 2 (Nrf2) and the subsequent increase in heme oxygenase-1 levels. Furthermore, ETME inhibited the LPS-induced production of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-1β, and IL-6. The inhibitory effects of ETME on pro-inflammatory responses were regulated by ETME-mediated dephosphorylation of mitogen-activated protein kinases (MAPKs: p38, p44/p42, and stress-associated protein kinase/c-Jun N-terminal kinase) and inhibition of nuclear localization of nuclear factor kappa B (NF-κB). These results suggest that ETME is a possible candidate for protecting Kupffer cells from LPS-mediated oxidative stress and excessive inflammatory responses by activating antioxidant Nrf2/HO-1 and inhibiting pro-inflammatory NF-κB and MAPKs, respectively.

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“…Yield (%) = Sample extract weight / Fresh plant part weight X 100 Bioassay guided fractionation Avasthi et al (2016) described a method for bioassayguided isolation of a dried cocoa leaf (CL) extract, which was modified slightly for the current study. The aim of the purification process was to identify the most potent cytotoxic bioactive fraction against MCF-7 cells using MTT assay (Figure 1).…”

Section: Cocoa Leaf Extract Preparationmentioning

confidence: 99%

Induction of Apoptosis and Modulation of Caspase Activity on MCF-7 Human Breast Cancer Cells by Bioactive Fractionated Cocoa Leaf Extract

Ranneh,

Abu bakar,

Md Akim

et al. 2023

Asian Pac J Cancer Prev

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Background:The objective of this study was to investigate the potential anti-proliferative activities of a methanolic extract of cocoa leaves (CL) obtained through sequential partition and fractionation against MCF-7 breast cancer cells. Methods: The methanolic extract of CL was partitioned in three separated solvents (hexane, dichloromethane, and methanol). Hexane partition was the most potent against MCF-7 cells growth with the lowest IC50 value. Then, it was subjected to two fractionation procedures, resulting in the identification of the CL bioactive fraction (II-F7) with potent toxicity against MCF-7 cells. Results: Further investigation into CL bioactive fraction (II-F7) revealed significant dose-dependent growth inhibitory effects on MCF-7 cells, which were attributed to the induction of apoptosis, as evidenced by the presence of apoptotic bodies, fragmented DNA, and disruption of mitochondrial membrane potential. Additionally, treatment with CL bioactive fraction (II-F7) upregulated the expression of pro-apoptotic genes (DDIT3, GADD45G and HRK) and significantly increased the activities of caspase-8 and caspase-9. Conclusion: Overall, this study suggests that bioactive fraction (II-F7) from CL extract has significant and selective cytotoxicity against MCF-7 cells through inducing apoptosis and has potential as a therapeutic agent for breast cancer treatment.

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Bioassay guided screening, optimization and characterization of antioxidant compounds from high altitude wild edible plants of Ladakh

Cited by 9 publications

References 20 publications

Medicinally active principles analysis of Tephrosia apollinea (Delile) DC. growing in the United Arab Emirates

Medicinally active principles analysis of Tephrosia apollinea (Delile) DC. growing in the United Arab Emirates

Inhibitory Effects of Ehretia tinifolia Extract on the Excessive Oxidative and Inflammatory Responses in Lipopolysaccharide-Stimulated Mouse Kupffer Cells

Induction of Apoptosis and Modulation of Caspase Activity on MCF-7 Human Breast Cancer Cells by Bioactive Fractionated Cocoa Leaf Extract

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