Bioassay of interleukin 1 (IL-1) in human gingival crevicular fluid during experimental gingivitis

Kinane, Denis F.; Winstanley, F. P.; Adonogianaki, E.; Moughal, N. A.

doi:10.1016/0003-9969(92)90011-v

Cited by 85 publications

(84 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of IL-1 in GCF has been shown by Charon et al (1982) and Kinane et al (1992), who identified higher levels in inflamed sites than in normal sites. The advent of more highly sensitive immunoassays for the detection of IL-1 has facilitated further studies relating to the nature of these cytokine molecules.…”

Section: Immunoglobulinsmentioning

confidence: 69%

Gingival Crevicular Fluid: Biomarkers of Periodontal Tissue Activity

Embery¹,

Waddington²

1994

Adv Dent Res.

View full text Add to dashboard Cite

The lack of precise clinical criteria for assessment of periodontal disease has led to a search for alternative means of determining active disease sites, prognosis of future sites of breakdown, and response to therapy. This review highlights the potential array of biomarkers present in gingival crevicular fluid and which may relate to existing or predicted tissue regions undergoing metabolic change and derived from bacterial or host-cell-derived products. Among the former may be listed endotoxin, amines, butyrate, and a variety of enzymes and their inhibitors, such as trypsin-like proteases and bacterial collagenase. Arising from host cells is a variety of leucocytic hydrolase enzymes, lactoferrin, and lysozyme. These appear to be useful inflammatory markers and may be distinguished from products of connective tissue breakdown which include collagenous and non-collagenous products, including collagen peptides, osteonectin, and fibronectin. The proteoglycans have found particular favor as biomarkers of possible bone-resorptive activity. Attention has also been directed at the immune response, including comment on immunoglobulins, complement, eicosanoids, and cytokines. This review lists available information on the presence of these in gingival sulcus fluid and wherever possible relates their presence to disease activity.

show abstract

Section: Immunoglobulinsmentioning

confidence: 69%

Gingival Crevicular Fluid: Biomarkers of Periodontal Tissue Activity

Embery¹,

Waddington²

1994

Adv Dent Res.

View full text Add to dashboard Cite

show abstract

“…The chronic interaction of the host immune system with subgingival plaque containing P. gingivalis and the RgpA-Kgp complexes in the subjacent tissue is believed to be a major factor in tissue destruction in chronic periodontitis (6,21,51,83). Compared with healthy subjects, gingival tissues and gingival crevicular fluid of patients with chronic periodontitis are reported to have significantly increased amounts of proinflammatory cytokines such as interleukin-1 (IL-1), IL-6, and tumor necrosis factor alpha (TNF-␣); chemokines such as IL-8, macrophage chemoattractant protein 1 (MCP-1), and macrophage inflammatory protein 1␣ (MIP-1␣); and adhesion molecules such as intracellular adhesion molecule 1 (ICAM-1) (12,25,27,28,32,33,37,44,46,62,84,87). These cytokines and chemokines play a significant role in mediating the recruitment of a dense mononuclear infiltrate, consisting mainly of T cells and macrophages (21,22,39,55,81).…”

mentioning

confidence: 99%

Porphyromonas gingivalisRgpA-Kgp Proteinase-Adhesin Complexes Penetrate Gingival Tissue and Induce Proinflammatory Cytokines or Apoptosis in a Concentration-Dependent Manner

et al. 2009

View full text Add to dashboard Cite

The RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis were observed, using immunostaining, in human gingival tissue associated with periodontitis but not in healthy tissue. The staining pattern suggested a concentration gradient from the subgingival plaque into the subjacent gingival connective tissue. Intense immunostaining was observed in areas displaying gross disturbance of tissue architecture. P. gingivalis cells and the RgpA-Kgp complexes at low concentrations were shown to stimulate secretory intercellular adhesion molecule 1, interleukin-8 (IL-8), IL-6, and macrophage chemoattractant protein secretion from cultured human epithelial (KB) and fibroblast (MRC-5) cells. However, at high concentrations a reduction in the level of these mediators was observed. In contrast, macrophage inflammatory protein 1␣ and IL-1␣ were stimulated only at high P. gingivalis cell concentrations. P. gingivalis cells and the RgpA-Kgp complexes were shown to induce apoptosis in KB and MRC-5 cells in a time-and dose-dependent manner. These data suggest that the RgpA-Kgp complexes penetrate the gingival connective tissue; at low concentrations distal from the plaque the complexes stimulate the secretion of proinflammatory mediators, while at high concentrations proximal to the plaque they induce apoptosis and attenuate the secretion of proinflammatory mediators.

show abstract

“…As there were no similar studies, we selected the deviation seen in CCL28 levels as reported by Ertugrul et al, 21 which was greater than in IL-1b levels reported in other studies. 18,20 On the basis of this data, the sample size was calculated to 17 subjects to enable detection of a difference of 20 ng/ml. However, considering the possibility of drop-out among subjects, the total number of patients was 20 for each group.…”

Section: Sample Sizementioning

confidence: 99%

“…18,19 A site specific increase in IL-1b has been observed in an experimental gingivitis model. 20 The chemokine (C-C motif) ligand 28 (CCL28) is a novel marker for the diagnosis of gingivitis and periodontitis. 21 Previous studies have shown CCL28 is associated with increased epithelial inflammation.…”

Section: Introductionmentioning

confidence: 99%

Effects of curcumin on crevicular levels of IL‐1β and CCL28 in experimental gingivitis

Pulikkotil

Nath²

2015

Australian Dental Journal

View full text Add to dashboard Cite

Background: Curcumin has anti-inflammatory properties. The aim of this study was to compare interleukin-1b (IL-1b) and chemokine (C-C motif) ligand 28 (CCL28) levels following a topical application of curcumin (CRM), chlorhexidine (CHX) and chlorhexidine-metronidazole (CHX-MTZ) in an experimental gingivitis human model. Methods: Sixty systemically healthy selected subjects were randomly assigned to one of three topical antigingivitis gels. Each gel was applied twice daily for 10 minutes as the sole method of oral hygiene for 29 days on the test quadrant only. Modified gingival index (MGI), plaque index (PI), bleeding on probing (BOP) and probing depth (PD) were assessed at baseline, 29 days and 60 days. Estimation of IL-1b and CCL28 levels in gingival crevicular fluid was done at baseline and at 29 days. Results: The increase of IL-1b in the CRM (14.52 AE 16.6 pg/ml) and CHX-MTZ (31.63 AE 15.96) groups was significantly less than that of the CHX group (70.55 AE 38.81). Similar results were also observed for CCL28 (CRM: 8.12 AE 8.78 pg/ml; CHX-MTZ: 12.81 AE 18.68; CHX: 41.15 AE 22.82). All groups had a significant increase in MGI, PI and BOP at 29 days. Conclusions: The anti-inflammatory potential of topical curcumin was similar to CHX-MTZ but superior to CHX in affecting IL-1b and CCL28 levels.

show abstract

Bioassay of interleukin 1 (IL-1) in human gingival crevicular fluid during experimental gingivitis

Cited by 85 publications

References 22 publications

Gingival Crevicular Fluid: Biomarkers of Periodontal Tissue Activity

Gingival Crevicular Fluid: Biomarkers of Periodontal Tissue Activity

Porphyromonas gingivalisRgpA-Kgp Proteinase-Adhesin Complexes Penetrate Gingival Tissue and Induce Proinflammatory Cytokines or Apoptosis in a Concentration-Dependent Manner

Effects of curcumin on crevicular levels of IL‐1β and CCL28 in experimental gingivitis

Contact Info

Product

Resources

About