Bioaugmentation and Adsorption Treatment of Coking Wastewater Containing Pyridine and Quinoline Using Zeolite-Biological Aerated Filters

Abstract

Bioaugmented zeolite-biological aerated filters (Z-BAFs), i.e. adding isolated degrading bacteria into the BAFs with zeolite as fillings, were designed to treat coking wastewater containing high concentrations of pyridine and quinoline and to explore the bacterial community of biofilm on the zeolite surface. The investigation was carried out for 91 days of column operation and the treatment of pyridine, quinoline, total organic carbon (TOC), and ammonium was shown to be highly efficient by bioaugmentation and adsorption. Biomass determination and bacterial diversity detection based on 16S rDNA and rRNA techniques supported the treatment data and indicated that bioaugmentation could recover the bacterial richness and diversity from pyridine and quinoline loading shocks. However, bioaugmentation accelerated the shift of the bacterial community structure resulting in a more distinct difference from the starting community. Clone library analysis revealed that pyridine and quinoline were more harmful to Bacterodietes among all ingenious bacteria, and bioaugmentation promoted the growth of Planctomycetes in the biofilm. Moreover, the introduced bacteria did not remain dominant in the bioaugmented biofilm, indicating the indigenous degrading bacteria played the most significant role in the treatment. This bioaugmented Z-BAF method was shown to be an alternative technology for the treatment of wastewater containing pyridine and quinoline or other N-heterocyclic aromatic compounds.

Treatment of groundwater containing Mn(II), Fe(II), As(III) and Sb(III) by bioaugmented quartz-sand filters

“…Most bioaugmentation studies on wastewater have revealed that the inoculated strain(s) may significantly alter indigenous microbial community composition (Bai et al, 2011;Shi et al, 2015;Wang et al, 2009;Zhu et al, 2015). In our study, however, the indigenous microbial community maintained their predominant structure after the introduction of strain QJX1.…”

Identification and removal of polycyclic aromatic hydrocarbons in wastewater treatment processes from coke production plants

“…Limitation on the study of PAHs in coking wastewater can be ascribed to many factors, including: (1) distribution of coking plants mainly in developing countries, which limits the study of representative wastewater samples; (b) economic and social conditions that do not allow developing countries to fully address pollutants from coking wastewater; and (c) technically difficult collection, extraction, and separation of target organic compounds from the complex matrix (Busetti et al 2006;Poster et al 2006). As economic development has proceeded and public health awareness has increased, recent research has begun to focus on coking wastewater (Zhu et al 2009;Bai et al 2011;Chu et al 2012). In recent years, many efforts have been made to develop effective analytical techniques for PAHs in complex matrices (Chen et al 2005;Busetti et al 2006;Guo et al 2007), which make the identification and quantitative analyses possible for PAHs in coking wastewater.…”

The Resilience of Microbial Community Involved in Coking Wastewater Treatment System

“…Illumina MiSeq high throughput sequencing technology was performed to reveal the relative abundance and diversity of microbiology in these two parallel systems. Since coking wastewater is usually regarded as an extreme environment with high toxicity and refractoriness, the microorganisms involved should be quite different from those in domestic wastewater [5]. But the microbial community which is responsible for the cooking wastewater treatment process is still significantly different?…”