Bioaugmentation of sidestream nitrifying-denitrifying phosphorus-accumulating granules in a low-SRT activated sludge system at low temperature

Figdore, Bryce; Stensel, H. David; Winkler, Mari‐Karoliina H.

doi:10.1016/j.watres.2018.02.035

Cited by 50 publications

(16 citation statements)

References 36 publications

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“…(here Nitrotoga) in wastewater treatment plants (WWTPs) (3,4). They mostly coexisted with Nitrospira but were also found to be the only NOB in some of these facilities for unknown reasons (4)(5)(6)(7). Additionally, Nitrotoga was detected in biofilters treating groundwater (8) as well as in freshwater and marine recirculation aquaculture systems (RASs) (9,10).…”

mentioning

confidence: 99%

Low Temperature and Neutral pH Define “ Candidatus Nitrotoga sp.” as a Competitive Nitrite Oxidizer in Coculture with Nitrospira defluvii

Wegen

Nowka

Spieck

2019

Appl Environ Microbiol

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Nitrification is an essential process for N removal in activated sludge to avoid toxicity of ammonium and nitrite. Besides Nitrospira, “Candidatus Nitrotoga” has been identified as a key nitrite-oxidizing bacterium (NOB) performing the second step of nitrification, nitrite oxidation to nitrate, in wastewater treatment plants (WWTPs). However, the driving forces for the dominance of Nitrotoga in certain plants have often remained unclear and could not be explained solely by temperature effects. In this study, we characterized the physiology of the ammonium-dependent Nitrotoga sp. BS with regard to temperature and pH variations and evaluated its competitiveness against Nitrospira defluvii. Both NOB originated from the same WWTP and shared a comparable pH optimum of 7.3. Based on these results, coculturing experiments with these NOB were performed in batch reactors operated at either 17°C or 22°C to compare their abundances under optimal (pH 7.4) or suboptimal (pH 6.4) conditions using 1 mM nitrite. As revealed by quantitative PCR (qPCR), fluorescence in situ hybridization (FISH), and 16S amplicon sequencing, Nitrotoga sp. BS was clearly favored by its optimal growth parameters and dominated over Ns. defluvii at pH 7.4 and 17°C, whereas a pH of 6.4 was more selective for Ns. defluvii. Our synthetic communities revealed that niche differentiation of NOB is influenced by a complex interaction of environmental parameters and has to be evaluated for single species. IMPORTANCE “Ca. Nitrotoga” is a NOB of high environmental relevance, but physiological data exist for only a few representatives. Initially, it was detected in specialized niches of low temperature and low nitrite concentrations, but later on, its ubiquitous distribution revealed its critical role for N removal in engineered systems like WWTPs. In this study, we analyzed the competition between Nitrotoga and Nitrospira in bioreactors and identified conditions where the K strategist Ns. defluvii was almost replaced by Nitrotoga sp. BS. We show that the pH value is an important factor that regulates the composition of the nitrite-oxidizing enrichment with a dominance of Nitrotoga sp. BS versus Ns. defluvii at a neutral pH of 7.4 in combination with a temperature of 17°C. The physiological diversity of novel Nitrotoga cultures improves our knowledge about niche differentiation of NOB with regard to functional nitrification under suboptimal conditions.

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mentioning

confidence: 99%

Low Temperature and Neutral pH Define “ Candidatus Nitrotoga sp.” as a Competitive Nitrite Oxidizer in Coculture with Nitrospira defluvii

Wegen

Nowka

Spieck

2019

Appl Environ Microbiol

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“…In the second form, another smaller separate bioreactor is used to grow cells especially selected or engineered to degrade specific compounds. (Cardinal and Stenstrom, 1991;Babcock et al, 1992;Figdore et al, 2018). Sidestream granular activated sludge to enhance nitrification and prompt phosphorus removal is another approach that can be used to produce a high-quality effluent (Figdore et al, 2018).…”

Section: Bioaugmentationmentioning

confidence: 99%

“…(Cardinal and Stenstrom, 1991;Babcock et al, 1992;Figdore et al, 2018). Sidestream granular activated sludge to enhance nitrification and prompt phosphorus removal is another approach that can be used to produce a high-quality effluent (Figdore et al, 2018). The effective removal of specific constituents through bioaugmentation has been demonstrated repeatedly (Cardinal and Stenstrom, 1991;Babcock et al, 1992;Nzila et al, 2016).…”

Section: Bioaugmentationmentioning

confidence: 99%

“…The process is simple to operate as there are no moving parts. The principal advantages are excellent removals of TSS, BOD, nitrogen, phosphorus, and other constituents; smaller footprint; secondary clarifiers not required; and energy savings due to reduced aeration requirements (de Kreuk and Loosdrecht, 2006;Figdore et al, 2017Figdore et al, , 2018Pronk et al, 2017).…”

Section: Implementation Of New Biological Treatment Processesmentioning

confidence: 99%

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Comprehensive Source Control for Potable Reuse

Tchobanoglous

Leverenz

2019

Front. Environ. Sci.

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“…The content of phosphorus in biologically treated wastewater decreases due to the sorption of phosphorus compounds, hydrolysis of complex and organic phosphates, and the consumption of phosphates by active sludge [10].…”

Section: Introductionmentioning

confidence: 99%

Special features of elimination of phosphorus compounds in aeration tanks

Zaletova

2020

E3S Web Conf.

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In the sewage treatment plants, phosphorus compounds presented constantly, due to the sources of their appearance. In urban wastewater, phosphorus stays in various compounds – dissolved, insoluble, organic, mineral, simple and complex forms. Only phosphates and total phosphorus are monitored in wastewater chemicals laboratories. The data on phosphorus compounds containing in the wastewater of a number of cities is analyzed in the study. The impact of the biological wastewater treatment process on the efficiency of phosphorus removal was assessed. The research was carried out on an experimental technological installation. It is shown that in the original wastewater not only the concentration of total phosphorus and phosphates is constantly varying, but also their ratio. In the process of biological treatment, a number of complex biochemical and physical-chemical processes are parallel: assimilation, hydrolysis, and adsorption. The effectiveness of these processes is influenced by the magnitude of the initial concentration of total phosphorus and the amount of active dose of sludge. It has been recognized that the increased efficiency of the removal of total phosphorus has an effect on both the increase in the active dose of sludge and the size of the initial concentration of total phosphorus. However, the residual concentration of total phosphorus is affected by its original content, while the initial content of the total phosphorus is affected insignificantly.

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Bioaugmentation of sidestream nitrifying-denitrifying phosphorus-accumulating granules in a low-SRT activated sludge system at low temperature

Cited by 50 publications

References 36 publications

Low Temperature and Neutral pH Define “ Candidatus Nitrotoga sp.” as a Competitive Nitrite Oxidizer in Coculture with Nitrospira defluvii

Low Temperature and Neutral pH Define “ Candidatus Nitrotoga sp.” as a Competitive Nitrite Oxidizer in Coculture with Nitrospira defluvii

Comprehensive Source Control for Potable Reuse

Special features of elimination of phosphorus compounds in aeration tanks

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