Bioaugmentation with an anaerobic fungus in a two-stage process for biohydrogen and biogas production using corn silage and cattail

Nkemka, Valentine Nkongndem; Gilroyed, Brandon H.; Yanke, Jay; Gruninger, Robert J.; Vedres, D. D.; McAllister, Tim A.; Hao, Xiying

doi:10.1016/j.biortech.2015.02.100

Cited by 113 publications

(39 citation statements)

References 28 publications

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“…It was noted, however, that sometimes such additional microorganisms added into the bioreactor fail to cause the desired biogas yield increase, and even lead to an inhibition of methane formation by anaerobic sludge [40,41]. The main reason for this failure was the difficulty in determining and supporting the necessary microbial equilibrium.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

et al. 2019

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The aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrifos or methiocarb and its derivatives). It was established that the immobilized cells of the anaerobic consortium can be stored frozen for at least three years while preserving a high level of metabolic activity. The cells after the long-term storage in an immobilized and frozen state were applied for the methanogenesis of a wide number of wastes, and an increase in both methane yield and methane portion in the produced biogas as compared to the conventionally used suspended anaerobic sludge cells, was ensured. It was shown that the “additional” introduction of bacterial Clostridium acetobutylicum, Pseudomonas sp., Enterococcus faecalis cells (also immobilized using same support) improves characteristics of methanogenesis catalyzed by immobilized anaerobic sludge.

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Section: Introductionmentioning

confidence: 99%

Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

et al. 2019

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“…Moreover, the results of previous studies in which AF cultures were inoculated into biogas digesters to improve biogas production rates showed that the AF were not able to persist in the biogas environment and died within the first 10 to 15 days after their implementation (Nkemka et al, 2015;Procházka et al, 2012). Taken together, the results suggest that at least the tested biogas fermenter environments with the given conditions do not favor AF growth and activity.…”

Section: 6mentioning

confidence: 77%

“…Attempts have been made to enhance biogas generation from plant biomass by addition of AF leading to higher biogas output (Procházka et al, 2012) and quicker initial H 2 and CH 4 production combined with improved volatile fatty acid degradation (Nkemka et al, 2015), principally demonstrating the potential of AF to improve fiber digestion. However, before bioaugmentation may be expanded to current full-scale biogas plants, it is first important to determine if AF are already present and particularly whether they are metabolically active in existing biogas reactors.…”

Section: Introductionmentioning

confidence: 99%

Presence and transcriptional activity of anaerobic fungi in agricultural biogas plants

Dollhofer¹,

Callaghan

Griffith

et al. 2017

Bioresource Technology

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Bioaugmentation with anaerobic fungi (AF) is promising for improved biogas generation from lignocelluloses-rich substrates. However, before implementing AF into biogas processes it is necessary to investigate their natural occurrence, community structure and transcriptional activity in agricultural biogas plants. Thus, AF were detected with three specific PCR based methods: (i) Copies of their 18S genes were found in 7 of 10 biogas plants. (ii) Transcripts of a GH5 endoglucanase gene were present at low level in two digesters, indicating transcriptional cellulolytic activity of AF. (iii) Phylogeny of the AF-community was inferred with the 28S gene. A new Piromyces species was isolated from a PCR-positive digester. Evidence for AF was only found in biogas plants operated with high proportions of animal feces. Thus, AF were most likely transferred into digesters with animal derived substrates. Additionally, high process temperatures in combination with long retention times seemed to impede AF survival and activity.

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“…Also, the temperature inside the digester strongly affects the biogas production process . When designing the course of a methane fermentation process, it is necessary to eliminate inhibitors like antibiotics or plant protection chemicals, which can substantially decrease the efficiency of the process …”

Section: Ad As a Revolutionary Process In Energy Generationmentioning

confidence: 99%

Intensification of biogas production using various technologies: A review

et al. 2020

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Summary Anaerobic digestion (AD) is an organic matter conversion technology which offers a wide range of options for production of biogas from organic biomass, providing an excellent opportunity to convert abundant bioresources into safe, eco‐friendly, renewable energy. Important factors in the process of AD are the biodiversity of microorganisms, chemical load of oxygen demand, and content of water and total solids. A challenge for the future is to find technologies that will maximally enhance biogas production and to find pathways for biogas to supersede well‐established technologies and practices in the contemporary heavily fossil fuel‐based energy system. Current studies on technologies of biogas production indicate a number of possibilities of using appropriate biological and physicochemical additives, like added enzymes or fruit pomace, as well as immobilizing microorganisms on biofilters. Anaerobic biorefinery is an emerging concept that generates not only bioenergy but also high‐value biochemical products from the same feedstock. This study is a review of articles describing the intensification of biogas production by using various technologies.

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Bioaugmentation with an anaerobic fungus in a two-stage process for biohydrogen and biogas production using corn silage and cattail

Cited by 113 publications

References 28 publications

Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas

Presence and transcriptional activity of anaerobic fungi in agricultural biogas plants

Intensification of biogas production using various technologies: A review

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