Bioaugmenting the poplar rhizosphere to enhance treatment of 1,4-dioxane

Simmer, Reid A.; Mathieu, Jacques; Silva, Marcio L. B. Da; Lashmit, Philip; Gopishetty, Sridhar; Alvarez, Pedro J. J.; Schnoor, Jerald L.

doi:10.1016/j.scitotenv.2020.140823

Cited by 18 publications

(5 citation statements)

References 59 publications

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“…During this work, we confirmed that through uptake and evapotranspiration, poplar trees alone can achieve low dioxane concentrations (∼1 μg/L) in the laboratory with simulated contaminated groundwater. We also demonstrated that bioaugmenting the rhizosphere with dioxane-metabolizing organisms, including Pseudnocardia dioxanivorans CB1190 and Mycobacterium dioxanotrophicus PH-06, speeds the treatment of dioxane . CB1190 can utilize root extract as an auxiliary carbon source, making it well equipped to colonize the poplar root zone .…”

Section: Bioaugmentation and Growth Factorsmentioning

confidence: 90%

“…We also demonstrated that bioaugmenting the rhizosphere with dioxane-metabolizing organisms, including Pseudnocardia dioxanivorans CB1190 and Mycobacterium dioxanotrophicus PH-06, speeds the treatment of dioxane. 59 CB1190 can utilize root extract as an auxiliary carbon source, making it well equipped to colonize the poplar root zone. 36 However, the root extract’s presence also caused catabolite repression in CB1190, slowing dioxane metabolism.…”

Section: Bioaugmentation and Growth Factorsmentioning

confidence: 99%

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Phytoremediation, Bioaugmentation, and the Plant Microbiome

Simmer

Schnoor

2022

Environ. Sci. Technol.

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Understanding plant biology and related microbial ecology as a means to phytoremediate soil and groundwater contamination has broadened and advanced the field of environmental engineering and science over the past 30 years. Using plants to transform and degrade xenobiotic organic pollutants delivers new methods for environmental restoration. Manipulations of the plant microbiome through bioaugmentation, endophytes, adding various growth factors, genetic modification, and/or selecting the microbial community via insertion of probiotics or phages for gene transfer are future areas of research to further expand this green, cost-effective, aesthetically pleasing technology�phytoremediation.

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Section: Bioaugmentation and Growth Factorsmentioning

confidence: 90%

Section: Bioaugmentation and Growth Factorsmentioning

confidence: 99%

Phytoremediation, Bioaugmentation, and the Plant Microbiome

Simmer

Schnoor

2022

Environ. Sci. Technol.

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“…Reactors were inoculated with 1 mL (1% total culture volume) of washed cells. Samples (3 mL) were taken regularly via serological pipet, sterile filtered (0.2 μm), and analyzed for dioxane (detailed below) and biomass as protein, as previously described . Following the experiment, the culture yield was calculated from the change in biomass per unit dioxane consumed during exponential growth in treatment reactors.…”

Section: Materials and Methodsmentioning

confidence: 99%

Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219

Simmer

Richards

Ewald

et al. 2021

Environ. Sci. Technol. Lett.

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Bioremediation is a promising treatment technology for 1,4-dioxane-contaminated groundwater. However, metabolic dioxane-degrading bacteria identified to date are limited by their slow kinetics and inability to sustain growth at low dioxane concentrations (<100 μg/L). Furthermore, strains may underperform because of missing growth factors, such as amino acids or vitamins. In this work, we reevaluate Rhodococcus ruber strain 219 as a dioxane-degrading strain with bioaugmentation potential. We report rapid growth and metabolic dioxane degradation by R. ruber 219 when supplemented with thiamine (vitamin B1). We also discern that the strain lacks a complete de novo thiamine synthesis pathway, indicating that R. ruber 219 is a probable thiamine auxotroph. However, when supplemented with thiamine, the strain’s Monod kinetics (K s = 0.015 ± 0.03 μg/L) and exceedingly low S min (0.49 ± 1.16 μg/L) suggest this strain can maintain growth at very low dioxane concentrations (<100 μg/L). Accordingly, we demonstrate that thiamine-grown R. ruber 219 sustains degradation of dilute dioxane (<100 μg/L) to below health advisory levels. This is the first study to report sustained metabolic dioxane biodegradation to below health advisory levels of 0.35 μg/L. Overall, our findings solidify R. ruber 219 as a promising candidate for bioremediation of dioxane-contaminated groundwater.

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“…Most scientific research focuses on microorganisms living on the surface of poplar roots, including the surrounding layer of soil, called the rhizosphere [7,8]. In addition to surface-inhabiting microorganisms, there are endophytic microorganisms, such as bacteria, that inhabit the internal tissues of poplars and do not cause disease or other negative effects on the plant [9].…”

Section: Introductionmentioning

confidence: 99%

Culturable Bacterial Endophytes of Wild White Poplar (Populus alba L.) Roots: A First Insight into Their Plant Growth-Stimulating and Bioaugmentation Potential

Gladysh,

Bogdanova,

Kovalev

et al. 2023

Biology

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The white poplar (Populus alba L.) has good potential for a green economy and phytoremediation. Bioaugmentation using endophytic bacteria can be considered as a safe strategy to increase poplar productivity and its resistance to toxic urban conditions. The aim of our work was to find the most promising strains of bacterial endophytes to enhance the growth of white poplar in unfavorable environmental conditions. To this end, for the first time, we performed whole-genome sequencing of 14 bacterial strains isolated from the tissues of the roots of white poplar in different geographical locations. We then performed a bioinformatics search to identify genes that may be useful for poplar growth and resistance to environmental pollutants and pathogens. Almost all endophytic bacteria obtained from white poplar roots are new strains of known species belonging to the genera Bacillus, Corynebacterium, Kocuria, Micrococcus, Peribacillus, Pseudomonas, and Staphylococcus. The genomes of the strains contain genes involved in the enhanced metabolism of nitrogen, phosphorus, and metals, the synthesis of valuable secondary metabolites, and the detoxification of heavy metals and organic pollutants. All the strains are able to grow on media without nitrogen sources, which indicates their ability to fix atmospheric nitrogen. It is concluded that the strains belonging to the genus Pseudomonas and bacteria of the species Kocuria rosea have the best poplar growth-stimulating and bioaugmentation potential, and the roots of white poplar are a valuable source for isolation of endophytic bacteria for possible application in ecobiotechnology.

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Bioaugmenting the poplar rhizosphere to enhance treatment of 1,4-dioxane

Cited by 18 publications

References 59 publications

Phytoremediation, Bioaugmentation, and the Plant Microbiome

Phytoremediation, Bioaugmentation, and the Plant Microbiome

Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219

Culturable Bacterial Endophytes of Wild White Poplar (Populus alba L.) Roots: A First Insight into Their Plant Growth-Stimulating and Bioaugmentation Potential

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