Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats

Linderborg, Kaisa M.; Kulkarni, Amruta; Zhao, Ai; Zhang, Jian; Kallio, Heikki; Magnússon, Jóhann D.; Haraldsson, Guđmundur G.; Zhang, Yumei; Yang, Baoru

doi:10.1016/j.foodchem.2018.12.130

Cited by 19 publications

(24 citation statements)

References 45 publications

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“…The fatty acid composition of the oils used in the feed is shown in Table 2 . The (n-3)-adequate diet group was also included as a control (referred to as normal feed group) in our earlier study evaluating the bioavailability of DHA from different stereospecific positions of triacylglycerols (TAGs) [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…Feces were collected as a pooled sample from the last 5 days as described earlier [ 38 ]. The fecal samples were weighed and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Gas chromatography was used to determine the fatty acid composition of the organs as described in [ 38 ]. Briefly, the fatty acid composition was determined with a Nexis GC-2030 (Shimadzu, Kyoto, Japan) with a flame ionization detector.…”

Section: Methodsmentioning

confidence: 99%

“…The compositional data were calculated using the correction factors from external standards and expressed as relative % of all FAs for plasma, feces, liver, brain, eye, testis, visceral fat, lung, and heart and as µg/100 mg of individual FA for feces. The compositions of the feces and plasma of the (n-3)-adequate group have been described also previously as the normal feed group [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

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Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats

Kulkarni

Zhao

Yang

et al. 2022

Foods

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The dietary intake of fatty acids (FAs) affects the composition and distribution of FAs in the body. Here, a first-generation (n-3)-deficiency study was conducted by keeping young (age 21 ± 2 days) Sprague–Dawley male rats on a peanut-oil-based diet for 33 days after weaning in order to compare the effect of mild (n-3)-deficiency on the lipid composition of different organs and feces. Soybean-oil-based diet was used as a control. The plasma FA levels corresponded to FAs levels in the organs. Lower docosahexaenoic acid (DHA) content was detected in the plasma, brain, testis, visceral fat, heart, and lungs of the (n-3)-deficient group, whereas the DHA content of the eye and feces did not differ between the experimental groups. The DHA content of the brains of the (n-3)-deficient group was 86% of the DHA content of the brains of the (n-3)-adequate group. The DHA level of the organs was affected in the order of visceral fat > liver triacylglycerols > lung > heart > liver phospholipids > testis > eye > brain, with brain being least affected. The low levels of (n-3) FAs in the liver, brain, eye, heart, and lung were offset by an increase in the (n-6) FAs, mainly arachidonic acid. These results indicate that, in rats, adequate maternal nutrition during pregnancy and weaning does not provide enough (n-3) FAs for 33 days of an (n-3)-deficient diet. Results of this study can be used also to evaluate the conditions needed to reach mild (n-3) deficiency in the first generation of rats and to evaluate the feasibility to collect data from a variety of organs or only selected ones.

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Section: Methodsmentioning

confidence: 99%

“…Feces were collected as a pooled sample from the last 5 days as described earlier [ 38 ]. The fecal samples were weighed and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats

Kulkarni

Zhao

Yang

et al. 2022

Foods

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“…The methylated samples were further analyzed by a Shimadzu Nexis GC-2030 gas chromatograph with an AOC-20i auto-injector and flame ionization detector (Shimadzu Corporation, Japan) equipped with an Agilent JandW GC column DB-23 (60 m × 0.25 mm i.d., liquid film 0.25 µm, Santa Clara, CA, USA) using helium as the carrier gas. The temperature program described by Linderborg et al (2019) was applied [23]. Supelco 37 Component FAME mix (Supelco, St. Louis, MO, USA) and FAME standard 68D (Nu-Check-Prep, Elysian, MN, USA) were used as the external standards.…”

Section: Chemical Propertiesmentioning

confidence: 99%

Predicting the Properties of Industrially Produced Oat Flours by the Characteristics of Native Oat Grains or Non-Heat-Treated Groats

Jokinen

Pihlava

Puganen

et al. 2021

Foods

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The aim of this study was to determine whether the properties of the native oat grain or non-heat-treated groats (laboratory-scale dehulling) can be used to predict the quality of the industrially produced oat flour produced from heat-treated groats. Quality properties such as the color, hectoliter weight, thousand seed weight and hull content of Finnish native grains (n = 30) were determined. Furthermore, the relationship between the properties of the native grains and the chemical composition of the raw oat materials before and after the milling process were studied. A significant relationship (p < 0.01) was observed between the thousand seed weight of the native oat groats and the chemical composition of the industrially produced oat flour. Furthermore, the protein content of the native grains measured by NIT correlated with the chemical composition of the oat flours. These results suggest that the properties of oat flour produced on an industrial scale, including heat treatment, could be predicted based on the properties of native oat grains.

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Fatty acid concentration and distribution in human milk: Comparison with infant formulas and animal milk

Yu,

Zhou,

Ding

et al. 2023

J Americ Oil Chem Soc

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The lipid profile including total fatty acid (FA), sn‐2 positional distribution on triacylglycerol, and phospholipid (PL) FA in human milk, infant formulas, and animal milk (cow, goat, camel, donkey, and yak milk) were analyzed. Animal milk contained higher saturated fatty acids (45.08%–66.29%) than human milk (37.44%) and infant formula (33.46%). In the perspective of FA, human milk and the commercial formulas showed a better similarity. Human milk contained significantly (p < 0.05) higher 16:0 at the sn‐2 position (50.88%) than infant formulas (23.09%) and animal milk (28.95%–46.72%). As to PL‐FA, human milk contained significantly (p < 0.05) higher 16:0 and 18:0 but lower 18:1 n‐9 than other milk. The relative contents of arachidonic acid (AA), docosahexaenoic acid (DHA), and nervonic acid (NA) in human milk were higher in PL‐FA than that in total FA. Noteworthy, the contents of AA, DHA, and NA in both total FA and PL‐FA were higher in human milk than other milk. These results indicated that the fortification of 16:0 at the sn‐2 position, and AA, DHA, and NA in both total FA and PL‐FA could improve the quality of infant formulas.

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Bioavailability of docosahexaenoic acid 22:6(n-3) from enantiopure triacylglycerols and their regioisomeric counterpart in rats

Cited by 19 publications

References 45 publications

Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats

Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats

Predicting the Properties of Industrially Produced Oat Flours by the Characteristics of Native Oat Grains or Non-Heat-Treated Groats

Fatty acid concentration and distribution in human milk: Comparison with infant formulas and animal milk

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